Phytochemical Characterization, Antioxidant and In Vitro Cytotoxic Activity Evaluation of Juniperus oxycedrus Subsp. oxycedrus Needles and Berries

Mrid, Reda Ben; Bouchmaa, Najat; Bouargalne, Youssef; Ramdan, Btissam; Karrouchi, Khalid; Kabach, Imad; Karbane, Miloud El; Idir, Abderrazak; Zyad, Abdelmajid; Nhiri, Mohamed

doi:10.3390/molecules24030502

Cited by 48 publications

(34 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, in our study, the stimulation of activity of the antioxidant enzymes at 400 µg/mL in context with the maximum cytotoxic effect of UBE at the same concentration on CAL 27 cell line could be explained by the production of a large amount of ROS in the tumor cells, which was induced by the dry extract [40]. The increased levels of the antioxidant enzymes activities can be explained as a defense strategy against the oxidative stress induced by UBE.…”

Section: Discussionsupporting

confidence: 75%

“…It is known that in the tumor cells exists a higher intrinsic oxidative stress than in the normal cells, due to their metabolic alterations; it was reported that the cancer cells have an important level of ROS, compared to the normal cells. This elevation of ROS may appear as result of an abnormal mitochondrial oxidative metabolism and can be responsible for the initiation and progression of different types of cancer [40]. However, the elevation of ROS to a great level may be lethal for tumor cells themselves [7].…”

Section: Discussionmentioning

confidence: 99%

“…However, the elevation of ROS to a great level may be lethal for tumor cells themselves [7]. In fact, several studies outlined the implication of elevated levels of H 2 O 2 in the induction of apoptosis, and their low concentration enhanced [40]. Therefore, the killing of cancer cells through the ROS or the oxidative stress causing-agents represent one of the theories proposed in the cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the Cytotoxic Activity of the Usnea barbata (L.) F. H. Wigg Dry Extract

et al. 2020

View full text Add to dashboard Cite

The secondary metabolites of lichens have proven to be promising sources of anticancer drugs; one of the most important of these is usnic acid, which is a phenolic compound with dibenzofuran structure that is responsible for the numerous biological actions of lichens of genus Usnea. As a result, in this study, we related to this phenolic secondary metabolite. The aim of the present study is the evaluation of the cytotoxic activity of Usnea barbata (L.) F. H. Wigg dry acetone extract (UBE). In advance, the usnic acid content was determined in various extracts of Usnea barbata (L.) F. H. Wigg: the liquid extracts were found in water, ethanol, acetone, and the dry acetone extract; the highest usnic acid quantity was found in the dry acetone extract. First, the cytotoxic action of UBE was assessed using Brine Shrimp Lethality (BSL) test; a significant lethal effect was obtained after 24 h of treatment at high used concentrations of UBE, and it was quantified by the high mortality rate of the Artemia salina (L.) larvae. Secondly, in vitro cytotoxicity of UBE was evaluated on human tongue squamous cells carcinoma, using CAL 27 (ATCC ® CRL-2095™) cell line. The most intense cytotoxic effect of UBE on CAL 27 cells was registered after 24 h; this response is directly proportional with the tested UBE concentrations. The obtained results have been reported regarding usnic acid content of UBE, and the data show that CAL 27 cells death was induced by apoptosis and high oxidative stress.Molecules 2020, 25, 1865 2 of 17 therapeutic tools for the total destruction of cancer cells with limited effects on normal cells [1]. It is known that carcinogenesis is a complex process, involving cellular and molecular alterations, mediated by endogenous and/or exogenous factors: oxidative DNA damage, chromosomal abnormalities, and oncogene activation are responsible for the development of cancer and may be induced by prolonged oxidative stress [2]. Thus, in cancer pathology, the reactive oxygen species (ROS) have dual role, confirmed by many studies: first, ROS are cytotoxic, having an important contribution in the etiology and progression of cancer [3]. At the same time, many antitumor agents can destroy the cancer cells through intense oxidative stress, generating ROS production in high quantities [4]. Increasing ROS levels through redox modulation could in the future be an effective strategy for the selective destruction of cancer cells (not normal cells) [5]; this method is called "oxidative therapy" [6], and it was developed by inducing cytotoxic oxidation as a stress factor in cancer cells in the treatment of cancer [7].One of the most invasive malignancies is the oral squamous cell carcinoma (OSCC), which is the most common cancer of oral cavity; it can affect any part of the oral cavity including lips, tongue, gums, buccal epithelium, and salivary glands [8]. Despite that, the oral cavity is easily accessible for the direct visual examination, and the mortality from oral cancer remains still high because in the early stages, the pat...

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Cytotoxic Activity of the Usnea barbata (L.) F. H. Wigg Dry Extract

et al. 2020

View full text Add to dashboard Cite

show abstract

“…As this work has been conducted at only 4 weeks, another study for the whole cycle of wheat should be conducted to validate this hypothesis. It is known that the glutathione-dependent system requires NADPH, as cofactor, to face reactive oxygen species resulted from the oxidative stress [27]. Therefore, low content of NADPH can disturb the cell response to these toxic compounds.…”

Section: Effect Of Ure On Plant Growth and Oxidative Stress Metabolism Of Wheat Plantsmentioning

confidence: 99%

The effect of foliar application of Ulva rigida extract on the growth and biochemical parameters of wheat plants

Latique

Mrid

Kabach³

et al. 2021

E3S Web Conf.

Self Cite

View full text Add to dashboard Cite

Many research studies have shown the importance of seaweed extract in agriculture. Marine macroalgae are rich in diverse compounds like lipids, proteins, carbohydrates, phytohormones, amino acids, osmoprotectants and minerals. In this study, we have tried to characterize Moroccan macroalgae species Ulva rigida collected from the coastal area of Akhfenir in order to evaluate their potential to improve growth plant. The proximate composition, mineral element (Ca, K, Mg, Na and Cl), pigments, amino acid, proteins, lipids, polyphenol contents were determined in liquid extract obtained from green seaweed (SWE) Ulva rigida. Results show that the application of SWE at different concentrations (0,12.5, 25, 50%) significantly enhanced growth parameters, especially with 25% of seaweed liquid extract. Therefore, algal treatment by foliar application is proved to be an effective technique to improve the growth of wheat plants. This study provides important information on the characterization, identification and utilization of seaweed resources for agriculture as biostimulants.

show abstract

“…Ethanolic extract of Juniperus turbinate was more potent cytotoxic than cisplatin in human breast adenocarcinoma MDA-MB-231 cell lines [357]. Juniperus oxycedrus ethanolic extract from needles and berries showed potent cytotoxic effects against two breast cancer cell lines (MDA-MB-468 and MCF-7), with no cytotoxicity towards normal cells (PBMCs) [358]. Satureja khuzistanica (Figure 7a (Araliaceae) showed antiproliferative actions on human MCF-7 breast cancer cells [359].…”

Section: Camptotheca Acuminate (Xǐ Shù or Happy Tree) Leavesmentioning

confidence: 99%

Alternative Treatments for Cancer Prevention and Cure [Part 1]

Mohiuddin¹

2019

APCT

View full text Add to dashboard Cite

Many lay people along with some so called "key opinion leaders" have a common slogan "There's no answer for cancer". Again, mistake delays proper treatment and make situation worse, more often. Compliance is crucial to obtain optimal health outcomes, such as cure or improvement in QoL. Patients may delay treatment or fail to seek care because of high out-of-pocket expenditures. Despite phenomenal development, conventional therapy falls short in cancer management. There are two major hurdles in anticancer drug development: dose-limiting toxic side effects that reduce either drug effectiveness or the QoL of patients and complicated drug development processes that are costly and time consuming. Cancer patients are increasingly seeking out alternative medicine and might be reluctant to disclose its use to their oncology treatment physicians. But there is limited available information on patterns of utilization and efficacy of alternative medicine for patients with cancer. As adjuvant therapy, many traditional medicines shown efficacy against brain, head and neck, skin, breast, liver, pancreas, kidney, bladder, prostate, colon and blood cancers. The literature reviews non-pharmacological interventions used against cancer, published trials, systematic reviews and meta-analyses.

show abstract

Phytochemical Characterization, Antioxidant and In Vitro Cytotoxic Activity Evaluation of Juniperus oxycedrus Subsp. oxycedrus Needles and Berries

Cited by 48 publications

References 42 publications

Evaluation of the Cytotoxic Activity of the Usnea barbata (L.) F. H. Wigg Dry Extract

Evaluation of the Cytotoxic Activity of the Usnea barbata (L.) F. H. Wigg Dry Extract

The effect of foliar application of Ulva rigida extract on the growth and biochemical parameters of wheat plants

Alternative Treatments for Cancer Prevention and Cure [Part 1]

Contact Info

Product

Resources

About