Phytochemical characterization, anti-cancer and antimicrobial activity of isolated fractions of <i>Alysicarpus vaginalis</i>

Abstract

&lt;p class="Abstract"&gt;The methanolic extract of &lt;em&gt;Alysicarpus &lt;/em&gt;vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for&lt;em&gt; in vitro&lt;/em&gt; antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC&lt;sub&gt;50 &lt;/sub&gt;values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and&lt;em&gt; E. coli&lt;/em&gt; whereas chloroform and n-butanol fractions were more effective against &lt;em&gt;S. aureus&lt;/em&gt; and &lt;em&gt;B. &lt;/em&gt;subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.&lt;/p&gt;

Bio‐fabrication of Silver Nanoparticles Using Alysicarpus vaginalis Extract: Preparation, Characterization and Comparative in vitro Antibacterial Evaluations

“…The crude alcoholic extract of A. vaginalis showed the presence of phytosterols, terpenoids, flavonoids, saponins, reducing sugar, glycosides and tannins, however the alkaloids were not found in the crude extract. The results are in agreement with the previous reports showing the presence of bioactive class of compounds in A. vaginalis extract [25,27,62] . A complex mixture of compounds with close Rf values were seen in the TLC of n ‐butanol fraction, hence pure compound could not be isolated.…”

Üçüncü basamak bir hastanede Legionella Pneumophila üriner antijen test sonuçlarının değerlendirilmesi

“…Ketoconazole was used as a positive control in this research. Again, the authors did not state the concentration of the positive control used in this research (24).…”

“…During colonoscopy mostly polyps and neoplastic lesions of the appendix due to the characteristics and location of the lesion by piecemeal resection can be completely resected with sufficient (3,6). The important point of resection was an invasion of the layers of submucosa and muscularis propria, protrusion of cecal wall, and removing the lesion with free margins (3) vaginalis (22,23,24).…”

“…Although, at this time there is more in vitro scientific evidence available. At present, antibacterial, anticancer, antifungal, antioxidant, and hepatoprotective properties of various parts of A. vaginalis have been detected (23)(24)(25)(26)(27)(28)(29)(30). Nonetheless, the antioxidant property has been reported by the majority number of studies.…”

“…Tetracycline was used as a positive control in this study. Besides, the authors did not state the concentration of tetracycline used in this study(24).…”