Phytochemical characterization and immunomodulatory effects of aqueous, ethanolic extracts and essential oil of Syzygium aromaticum L. on human neutrophils

Faqer, Othman El; Bendiar, Salma; Rais, Samira; Elkoraichi, Ismail; Dakir, Mohamed; Elouaddari, Anass; Elamrani, Abdelaziz; Oudghiri, Mounia; Mtairag, El Mostafa

doi:10.1016/j.sciaf.2022.e01395

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…It was collected during the full flowering period on May 3, 2019 in Taza -Morocco (Latitude 33.938848 and Longitude -4.305174). The identification of the plant was realized as described in our previous work (19). A specimen was deposited under certificate number LN 05062019 for Laurus nobilis L. Briefly, the leaves aqueous and ethanolic extracts were prepared respectively, by infusion and maceration of the powdered leaves of L. nobilis (50 g each) with 500 mL of the appropriate solvents.…”

Section: Preparation Of Plant Materials and Extractsmentioning

confidence: 99%

“…A specimen was deposited under certificate number LN 05062019 for Laurus nobilis L. Briefly, the leaves aqueous and ethanolic extracts were prepared respectively, by infusion and maceration of the powdered leaves of L. nobilis (50 g each) with 500 mL of the appropriate solvents. The extraction procedure was further continued according to the described scheme in our previous work (19).…”

Section: Preparation Of Plant Materials and Extractsmentioning

confidence: 99%

“…The quantitative high-performance liquid chromatography (HPLC) assay of L. nobilis extracts was carried out using the HPLC technique as described in our previous work using the same equipment and a fully validated chromatographic procedure (19).…”

Section: Identification Of Phenolic Compounds By Hplcmentioning

confidence: 99%

“…The chemical analysis of EO was realized using GC/MS technique as described in our previous work (19).…”

Section: Essential Oil Analysismentioning

confidence: 99%

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Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils

Faqer

Rais

Elkoraichi

et al. 2022

J Herbmed Pharmacol

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Introduction: The leaves of Moroccan bay laurel (Laurus nobilis L.) have been used in several forms of extracts to cure rheumatic pain due to their anti-inflammatory properties. Our work aimed to evaluate the effects of aqueous and ethanolic extracts, as well as the essential oil (EO) from laurel, on the microbicidal activity of human neutrophils when compared to the effect of eucalyptol. Methods: The extracts (ethanolic and aqueous) were subject to phytochemical profiling and high-performance liquid chromatography (HPLC) analyses. The EO obtained by hydrodistillation from laurel was analyzed by gas chromatography-mass spectrometry (GC-MS). The immunomodulatory effects on neutrophil microbicidal activity of the extracts, EO, and eugenol were carried out by 3-(4,5-diméthylthiazol-2-yl)-2,5-diphényltétrazolium (MTT) assay. Results: The phytochemical analysis of the extracts revealed the presence of flavonoids, coumarins, phenols, flavone aglycones, and tannins. HPLC analysis showed the presence of numerous phenolic molecules such as syringic acid, ferulic acid, gallic acid, caffeine, and quercetin. The chemical composition of EO revealed that the major components were eucalyptol (44.14%), α-terpinyl acetate (11.11%), and β-phellandrene (6.74%). Aqueous and ethanolic extracts and EO revealed a significant and dose-dependent ability to inhibit neutrophils microbicidal activity with maximal inhibition at 200 µg/mL concentration with 30.42%, 24.7%, and 38.13%, respectively (P<0.001). Conclusion: The obtained results revealed the immunomodulatory properties of laurel as a potential natural anti-inflammatory agent that would also allow the development of new anti-inflammatory drugs.

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Section: Preparation Of Plant Materials and Extractsmentioning

confidence: 99%

Section: Preparation Of Plant Materials and Extractsmentioning

confidence: 99%

Section: Identification Of Phenolic Compounds By Hplcmentioning

confidence: 99%

“…The chemical analysis of EO was realized using GC/MS technique as described in our previous work (19).…”

Section: Essential Oil Analysismentioning

confidence: 99%

See 2 more Smart Citations

Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils

Faqer

Rais

Elkoraichi

et al. 2022

J Herbmed Pharmacol

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“…Sample and polyphenol standards were injected at a volume of 20 µL. The elution flow rate was fixed at 1 mL/min as mentioned by (6). Synthetic polyphenols like Gallic, vanillin, ferulic acids, quercetin, and catechin from (Sigma Chemical Company, USA) were used as standards.…”

Section: Hplc Analysismentioning

confidence: 99%

In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.

Elkoraichi

Latif

Faqer

et al. 2022

J Herbmed Pharmacol

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Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because of their richness in secondary molecules. The following study analyses the composition of the aqueous extract of Punica granatum peel and evaluates in vivo and in vitro antioxidant effects, hemolytic protection, and acute toxicity. Methods: Quantification of the plant extract was realized by high-performance liquid chromatography (HPLC). The hemolytic assay was used for erythrocyte protection, while the in vitro antioxidant effect was evaluated by 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) and reducing ferric power (RFP) assays. The in-vivo antioxidant activity was tested by measuring levels of lipid peroxidation (LPO) in serum. The toxicological study was tested by oral administration of the extract to four groups of mice for 21 days, followed by a histopathological examination of the spleen. Results: HPLC analysis showed the presence of some phenolic compounds such as coumarin, caffeic, gallic and syringic acids. The IC50 of the antioxidant assays were 254.49 ± 62.17 μg/mL and 40.265 ± 2.9 μg/mL for ABTS and reducing power, respectively. Furthermore, the thiobarbituric acid reactive substances (TBARS) assay showed the lowest levels at 150 mg/mL concentration. All of the concentrations used for hemolytic protection did not exceed 15% of hemolysis. Moreover, the toxicity test showed no sign of mortality, signs of weakness, or weight loss; also the histopathological examination of the spleen tissues showed the absence of any damage. Conclusion: The peel extract of P. granatum showed good potential and could be exploited as a natural antioxidant and antihemolytic remedy, leading to the development of new drugs.

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Phytochemical analysis and immunomodulatory activities in vitro and in vivo of Aframomum melegueta K Schum seed extracts

Latif,

Elkoraichi,

El Faqer

et al. 2024

Inflammopharmacol

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Phytochemical characterization and immunomodulatory effects of aqueous, ethanolic extracts and essential oil of Syzygium aromaticum L. on human neutrophils

Cited by 6 publications

References 27 publications

Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils

Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils

In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.

Phytochemical analysis and immunomodulatory activities in vitro and in vivo of Aframomum melegueta K Schum seed extracts

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