Phytochemical characterisation and bioprospection for antibacterial and antioxidant activities of Lippia alba Brown ex Britton & Wilson (Verbenaceae)

Abstract

Ethanol extract and fractions obtained from fresh and dry aerial parts of Lippia alba were examined in order to determine their phytochemical composition, antioxidant capacity and antibacterial activities. The ethanol extracts and fractions exhibited an antioxidant effect by the DPPH assay, especially samples of fresh plant. HPLC analysis of the ethyl acetate fractions identified the presence of phenolic acids and flavonoids. The ethanol extract and fractions showed activity against reference and multidrug-resistant strains of Staphylococcus aureus and Enterococcus faecalis (MIC range 2000-250 μg/mL). The hexane and dichloromethane fractions of fresh plant showed better activity against reference strains of Escherichia coli (MIC of 250 and 125 μg/mL, respectively), but all extracts and fractions were less active against multidrug-resistant strains of all the Gram-negative species evaluated. The results showed that the extract and fractions of L alba aerial parts showed antibacterial activity, even against multidrug-resistant Gram-positive bacteria, and antioxidant effect (DPPH assay).

In Vitro Antioxidant, Anticancer and Anti-cytotoxic Activity of Lippia alba

“…In their study, both extracts with fresh plant and those with dried plant, the highest flavonoid content was observed in the ethyl acetate fraction, followed by the ethanolic fraction. In comparison to the study by Oliveira et al [27], the crude ethanol extract of our study showed a higher total flavonoid content than the ethanol fraction obtained from dried plants (10.01 mg/g). In addition, according to Poyer and Schaefer [28], the total tannin content in the hydroalcoholic extract of the leaves of L. alba oscillates between 1.03 and 2.40 mg/g, values lower than those observed in our study (18.42 mg/g).…”

“…Phytochemistry screening showed the presence alkaloids, tannins and flavonoids in L. alba aqueous extract [26]. Oliveira et al [27] investigated fresh and dried L. alba ethanol extract fractions. In their study, both extracts with fresh plant and those with dried plant, the highest flavonoid content was observed in the ethyl acetate fraction, followed by the ethanolic fraction.…”

Antioxidant activity of essential oils from condiment plants and their effect on lactic cultures and pathogenic bacteria

“…RIcal oils (OLIVEIRA et al, 2013;EKPENYONG et al, 2015;AVOSEH et al, 2015;ALI et al, 2017). Different chemotypes of C. citratus had different major compounds, besides showing remarkably higher percentage of volatile geranial and neral terpenoids in species distributed in Brazil (GUIMARÃES et al, 2011) and in other countries (AVOSEH et al, 2015).…”

Infusiones de Moringa oleifera (moringa) combinada con Cymbopogon citratus (hierba luisa) y Lippia alba (mastranto)

“…En el extracto de las hojas de C. citratus se ha reportado la presencia de metabolitos de naturaleza fenólica como: ácido cafeico, ácido Nambiar y Matela, 2012;Shah et al, 2012). En relación a las hojas de L. alba se informa la presencia de apigenina, catequina, epicatequina, kaempferol, luteolina, naringina, quercetina, rutina y taxifolina (Hennebelle et al, 2008;Chies et al, 2013;Teixeira et al, 2018).…”

“…(Guzmán, et al, 2004). Los aceites esenciales han revelado actividades antivirales, antibacterianas, antifúngicas y antiparasitarias (Pino-Alea et al, 1996;Andrighetti-Fröhner et al, 2005;Teixeira-Duarte et al, 2005;Mesa-Arango et al, 2009). Efectos sedantes han sido atribuidos a flavonoides e iridoides (Zétola et al, 2002, Hennebelle et al, 2008, Thierry et al, 2008.…”