Phytochemical, biochemical, and growth changes in response to salinity in callus cultures of Nigella sativa L.

Golkar, Pooran; Bakhshi, Golab; Vahabi, Mohammad Reza

doi:10.1007/s11627-020-10058-z

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…The increased MDA content in salinity-stressed calli has been reported in other species as sa ower (Golkar and Taghizadeh 2018) Nigella sativa (Golkar et al 2020) and Acanthophyllum (Niknam et al 2011). In diploid genotypes, the amounts of MDA showed an increase with an increase in the salinity levels except for bra2828 and bra258 under 75 mM NaCl which had a signi cant decrease compared to the control (Table 2).…”

Section: Malondialdehide (Mda) Contentsupporting

confidence: 70%

“…This phenomenon could be resulted from water potential gradient between the nutrient medium and cell (Lokhande et al 2010). This negative potential would result in a decrease of the growth rate of the calli in dehydrated cells under salinity stress (Golkar et al 2020). The ndings demonstrated that the reduction in callus growth was more severe in diploid genotypes rather than that of the amphidiploid ones (Fig.…”

Section: Callus Growth Traitsmentioning

confidence: 99%

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Effect of salt stress on some physio-biochemical traits and antioxidative enzymes of two Brassica species under callus culture

Shahbazi

Jamei²,

Meratan

et al. 2021

Plant Cell Tiss Organ Cult

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The changes in lipid peroxidation, H 2 O 2 , proline, protein and the involvement of the different antioxidant system (catalase, gayacoule peroxidase, ascorbate peroxidase) and callus-related traits were investigated in relation to salt stress in the callus of two different ploidy levels of Brassica including B. juncea and B. oleracea. The calluses of B. juncea genotypes were less sensitive to NaCl stress than that of B. oleraceae by increasing concentrations of NaCl from 0 to 200 mM.Tetraploid genotype (B. juncea cr3356) showed signi cant increase in the contents of protein and proline, and also activity of giacle peroxidase and catalase enzymes at higher salinity levels. Also, a signi cant decrease in the amount of H 2 O 2 and malondialdehyde occurred with increasing the salinity intensity. Diploid cultivar (B. oleracea bra 2828) had the lowest enzymatic activities and the highest content of H 2 O 2 and malondialdehyde with an increase in the salinity level. Therefore, this genotype was identi ed as the most sensitive cultivar to the salinity stress. The difference in the salinity resistance between diploid and amphidiploid species may be due to differences in the ploidy level of these species. This result suggests that tetraploid genome of B. oleraceae could be considered as a suitable candidate for production under salinity conditions by maintaining higher activities of antioxidant enzymes.

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Section: Malondialdehide (Mda) Contentsupporting

confidence: 70%

Section: Callus Growth Traitsmentioning

confidence: 99%

Effect of salt stress on some physio-biochemical traits and antioxidative enzymes of two Brassica species under callus culture

Shahbazi

Jamei²,

Meratan

et al. 2021

Plant Cell Tiss Organ Cult

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“…Figure 6. Where phenolic and flavonoid content correlates with antioxidant nature, DPPH assay is a nonenzymatic antioxidant activity which is a direct and sensitive method to investigate savaging of ROS by antioxidants (Golkar et al, 2020). In the present study, DPPH radical scavenging activity significantly increased in both shoot and root as the salt concentration increased from 75-125 mM NaCl in 'PKU-AKM 12-28' and 'PKU-AKM 8802' (Figure 6E-6F).…”

Section: Effect Of Salt Stress On Protein Contentsupporting

confidence: 51%

Morphological, physiological, and biochemical responses to NaCl-induced salt stress in mungbean (Vigna radiata L.) varieties

Mankar¹,

Wayase²,

Shelke³

et al. 2021

Not Sci Biol

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Seventeen mungbean varieties [Vigna radiata (L.) R. Wilczek] were subjected to 100-400 mM salinity stress at the germination stage, and the indices of seed germination and early seedling growth were analysed. With the increasing salinity, seed germination and seedling growth attributes were affected in all varieties. Principal component analysis and hierarchical cluster analysis of varietal responses on the germination and seeding growth attributes at 400 mM NaCl separated seventeen varieties into four distinct clusters. Principal component analysis at lower salt stress levels indicated that the attributes of germination and early seedling growth are reliable to identify salt-tolerant mungbean varieties. In contrast, only germination attributes are reliable at higher salinity levels. Two salt-susceptible and salt-tolerant varieties were further assessed for NaCl-induced physiological and biochemical changes. Levels of proteins, secondary metabolites, osmolyte, and antioxidants were increased at lower salt concentrations but reduced at higher salt concentrations. Photosynthetic pigments decreased and membrane damage increased under salinity. Varieties that showed tolerance to salt stress can be used in salinity-affected agriculture fields after validating their salt tolerance in field experiments.

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“…Our finding indicated that flavonols components were affected more at salinity conditions rather than drought stress. In the literature, Golkar et al (2020) have also recorded a similar increase of antioxidant activity in N. sativa callus treated by intensive salinity related to the enhancement of total anthocyanin's concentrations and the total flavonols content. Some studies indicated no difference between rutin and quercetin under drought stress Gray et al (2003): Hypericum perforatum).…”

Section: Discussion Discussion Discussionmentioning

confidence: 59%

“…Efforts conducted to explain salinity or drought stress on secondary metabolites of this plant are very limited. However, different researchers studied the effect of salinity on fatty acids, phenolics, and antioxidant activity of this medicinal plant (Bourgou et al, 2010(Bourgou et al, , 2012Bensalem et al, 2020;Golkar et al, 2020;Iqbal et al, 2021). The impact of drought stress on phytochemical components was investigated by several studies (Bayati et al, 2020;Ghamarnia and Jalili, 2013;Mozzafari et al, 2000).…”

mentioning

confidence: 99%

Alterations in leaf anatomy, quality, and quantity of flavonols and photosynthetic pigments in Nigella sativa L. subjected to drought and salinity stresses

Aghajanzadeh-Gheshlaghi

Peyvandi

Majd

et al. 2021

Not Bot Horti Agrobo

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Nigella sativa was widely used for nutritional and medicinal purposes. The present study investigated the effect of drought and salinity stresses on anatomical leaves structure and some biochemical properties to increase the secondary metabolites. For salt stress plants were treated with NaCl (30, 60 mM), and for drought stress plants were irrigated daily (control), once every two days (2DI) and once every three days (3DI). Compared to control plants, 2DI, 3DI, and NaCl 60 mM treatments increase significantly leaf rutin content, while the amount of rutin in seeds of NaCl 60 mM treated plants showed a significant decrease. 3DI treatment also significantly increased rutin content in seeds compared to NaCl 30 mM and control plants. The maximum level of quercetin (0.58 mg g−1 DW), kaempferol (0.16 mg g−1 DW), and myricetin (0.04 mg g−1 DW) in leaves were gained in both NaCl treatments. However, the flavonol components were affected more at salinity conditions rather than drought. In all treated plants, the amount of these compounds in leaves was more than in seeds. The highest amount of total phenol (130 mg g−1 DW), flavonoids (11.4 mg g−1 DW), and carotenoid content (1.55 mg g−1 DW) of leaves were observed under 2DI stress. Treated plants probably encountered different changes in the anatomical structure of leaves, including the decrease of phloem area, reducing vascular bundles and diameters, decreasing the number, and increasing the volume of cortex cells. The study also corroborates the cooperation between increasing the antioxidant capacity with the total flavonoid, rutin, and quercetin. Results indicated a higher sensitivity of N. sativa to drought stress than salinity stress and indicated that moderate salinity and drought could enhance secondary metabolites of seeds in this plant. The formation of potent antioxidants via the treatments could be worthy for pharmaceutical industries.

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Phytochemical, biochemical, and growth changes in response to salinity in callus cultures of Nigella sativa L.

Cited by 14 publications

References 43 publications

Effect of salt stress on some physio-biochemical traits and antioxidative enzymes of two Brassica species under callus culture

Effect of salt stress on some physio-biochemical traits and antioxidative enzymes of two Brassica species under callus culture

Morphological, physiological, and biochemical responses to NaCl-induced salt stress in mungbean (Vigna radiata L.) varieties

Alterations in leaf anatomy, quality, and quantity of flavonols and photosynthetic pigments in Nigella sativa L. subjected to drought and salinity stresses

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