Phytochemical, antioxidant and anticancer activities of extracts of seven fruits found in the Southern Brazilian flora

Bagattoli, P.C.D.; Cipriani, DC; Mariano, Luísa Nathália Bolda; Corrêa, Maria Elinete; Wagner, TM; Noldin, VF; filho, VCechinel; Niero, Rivaldo

doi:10.4103/0250-474x.180239

Cited by 23 publications

(1 citation statement)

References 24 publications

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“…An inhibition of less than 50% could indicate low selectivity, which would be consistent with our results. Antiviral activity has not been reported for the other plants in Group A, but antioxidant activity has been reported for extracts from D. inconstans [48], and antitumor [49] and antifungal activities [50] have been documented for extracts from Ch. icaco .…”

Section: Discussionmentioning

confidence: 99%

In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting

Trujillo-Correa

Quintero-Gil

Díaz-Castillo

et al. 2019

BMC Complement Altern Med

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BackgroundFor decades, bioprospecting has proven to be useful for the identification of compounds with pharmacological potential. Considering the great diversity of Colombian plants and the serious worldwide public health problem of dengue—a disease caused by the dengue virus (DENV)—in the present study, we evaluated the anti-DENV effects of 12 ethanolic extracts derived from plants collected in the Colombian Caribbean coast, and 5 fractions and 5 compounds derived from Psidium guajava.MethodsThe cytotoxicity and antiviral effect of 12 ethanolic extracts derived from plants collected in the Colombian Caribbean coast was evaluated in epithelial VERO cells. Five fractions were obtained by open column chromatography from the ethanolic extract with the highest selectivity index (SI) (derived from P. guajava, SI: 128.2). From the fraction with the highest selectivity (Pg-YP-I-22C, SI: 35.5), five compounds were identified by one- and two-dimensional nuclear magnetic resonance spectroscopy. The antiviral effect in vitro of the fractions and compounds was evaluated by different experimental strategies (Pre- and post-treatment) using non-toxic concentrations calculated by MTT method. The DENV inhibition was evaluated by plate focus assay. The results were analyzed by means of statistical analysis using Student’s t-test. Finally the antiviral effect in Silico was evaluated by molecular docking.ResultsIn vitro evaluation of these compounds showed that three of them (gallic acid, quercetin, and catechin) were promising antivirals as they inhibit the production of infectious viral particles via different experimental strategies, with the best antiviral being catechin (100% inhibition with a pre-treatment strategy and 91.8% with a post-treatment strategy). When testing the interactions of these compounds with the viral envelope protein in silico by docking, only naringin and hesperidin had better scores than the theoretical threshold of − 7.0 kcal/mol (− 8.0 kcal/mol and − 8.2 kcal/mol, respectively). All ligands tested except gallic acid showed higher affinity to the NS5 protein than the theoretical threshold.ConclusionEven though bioprospecting has recently been replaced by more targeted tools for identifying compounds with pharmacological potential, our results show it is still useful for this purpose. Additionally, combining in vitro and in silico evaluations allowed us to identify promising antivirals as well as their possible mechanisms of action.

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Section: Discussionmentioning

confidence: 99%

In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting

Trujillo-Correa

Quintero-Gil

Díaz-Castillo

et al. 2019

BMC Complement Altern Med

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Myrciaria Genus: Bioactive Compounds and Biological Activities

Pereira

Charret

Machado

et al. 2022

Chemistry & Biodiversity

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The Myrtaceae family is of angiosperms, imposing its size and economic, cultural, and scientific importance. The genus Myrciaria, belonging to this family, has 33 species currently accepted, many of which are research targets aimed at elucidating their bioactive compounds and biological activities. Most species of the Myrciaria genus have terpenes in their composition, mainly mono and sesquiterpenes, and phenolic compounds such as tannins, phenolic acids, and flavonoids. Other secondary metabolites are also observed, such as alkaloids, steroids, coumarins, saponins, and naphthoquinones. These bioactive compounds are closely related to these species′ most diverse biological activities: antioxidant, anti‐inflammatory, analgesic, antiproliferative, antimicrobial, antiparasitic, insecticide, metabolic, protective, and nutraceutical. This work aims to provide a review of secondary metabolites and medicinal properties related to the genus Myrciaria, thus stimulating further studies on the species of this genus.

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Evaluation of the Antiproliferative Potential of Yellow Jaboticaba (Myrciaria glazioviana) Extracts Against Human Cervical Cancer (HeLa cells line) and the Analysis of Their Chemical Composition by HPLC‐HRESIMS

Toledo Martins Pereira,

Sardou Charret,

Freimann Wermelinger

et al. 2024

Chemistry & Biodiversity

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There is a high demand for new effective antineoplastic drugs with few side effects. In this sense, recent research highlights the potential of compounds of natural origin in treating and preventing different types of cancer. This study aims to evaluate the anticancer activity of the dichloromethane extract (MGD) and ethyl acetate extract (MGA) of Myrciaria glazioviana leaves against human cervical cancer cell line (HeLa), as well as to identify their bioactive compounds. Using the HPLC‐HRESIMS technique, ten compounds were characterized in both samples: quinic acid, ellagic acid, Tri‐O‐methyl ellagic acid, two derivatives of Tetra‐O‐methyl flavellagic acid, quercetrin, Di‐O‐methyl ellagic acid, and three derivatives of pentamethyl coruleoellagic acid. Through MTT assays using HeLa cells and NIH/3T3 cells, it was observed that MGD and MGA were selective against tumor cells, with IC50 values of 24.31 and 12.62 µg/mL, respectively. The samples induced tumor cell death by apoptosis, as evidenced by the activation of caspases 3/7, cell shrinkage, and pyknotic nuclei. Both samples were also able to inhibit the migration of HeLa cells after 24 hours of treatment, indicating a potential antimetastatic effect. Therefore, the present research highlights the antiproliferative and antimigratory potential of this species against HeLa cells.

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Phytochemical, antioxidant and anticancer activities of extracts of seven fruits found in the Southern Brazilian flora

Cited by 23 publications

References 24 publications

In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting

In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting

Myrciaria Genus: Bioactive Compounds and Biological Activities

Evaluation of the Antiproliferative Potential of Yellow Jaboticaba (Myrciaria glazioviana) Extracts Against Human Cervical Cancer (HeLa cells line) and the Analysis of Their Chemical Composition by HPLC‐HRESIMS

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