Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers

Chigayo, Kayini; Mojapelo, Paul Eanas Lesedi; Mnyakeni-Moleele, Simon S.; Misihairabgwi, Jane M.

doi:10.1016/j.apjtb.2016.10.004

Cited by 76 publications

(50 citation statements)

References 35 publications

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“…Studies by Ibrahim et al, [23] shows the presence of some bioactive compounds like tannin, alkaloid, terpenoids, flavonoid and saponin for both methanolic and aqueous extractions for mimosa pudica leaves, this corroborates our findings in this study for A. djalonensis. The result obtained for A. djalonensis for methanol and aqueous extracts show the presence of phenol, flavonoid and tannin, with the absence of terpenoids and cardiac glycosides in the ethylacetate extract, this is synonymous to the findings of Chigayo et al, [24]. Most of these phytochemicals serve as natural antibiotics, which assist the body in fighting microbial invasion and broad spectrum infections [25].…”

Section: Discussionmentioning

confidence: 77%

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

Popoola

2019

Int. Ann. Sci.

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The choice of suitable solvent is essential in the extraction of much needed bioactive phytochemicals present in medicinal plants. This study assessed the phytochemical status of four solvents (methanol, hexane, ethylacetate and aqueous) extracts of Anthocleista djalonensis, leaves of the plants were extracted with the solvents by cold maceration for three days; screening and quantitative determination of saponin, alkaloid, tannin, cardiac glycosides, flavonoids phenols and terpenoids was done using standard methods. Phytochemical screening indicates the presence of all the phytocompound in the methanol extract, while other extracts shows variation in the availability of different phytochemicals, quantitative results shows alkaloid ranged from 1.79-18.90 mg/100g, flavonoids: 2.54-10.53 mg/100g, saponin: 2.00-12.23 mg/100g, tannin: ND-4.83 mg/100g, phenol: ND-6.65 mg/100g, cardiac glycoside: ND-2.08 mg/100g, and terpenoids: ND-1.98 mg/100g; significant difference (p<0.05) exist in each phytochemicals across the solvents where methanol and the aqueous extracts gave the best extraction efficiency for the phytochemicals. Consequently, the optimum extraction of useful phytochemicals from A. djalonensis could be achieved using either methanol and water.

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Section: Discussionmentioning

confidence: 77%

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

Popoola

2019

Int. Ann. Sci.

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“…Also, the sterical structures of antioxidants or free radicals are known to play a more important role in their abilities to scavenge different types of free radicals. 27 When making a comparison between the yields of extracts, it was found that the polar solvents gave better extraction yields, which is true in the study of Chigayo et al 28 Since biologically active compounds occur naturally in very small concentrations, the choice of an extraction method and the corresponding suitable solvent is an important step in the drug discovery process. Addition of water to methanol proved that the extraction efficiency can be increased significantly as the extraction yields obtained with 80% methanol (52.9% yield) and 60% methanol (46.9% yield) were much higher than the yield obtained from the use of pure methanol (36.6% yield).…”

Section: Discussionmentioning

confidence: 96%

“…Addition of water to methanol proved that the extraction efficiency can be increased significantly as the extraction yields obtained with 80% methanol (52.9% yield) and 60% methanol (46.9% yield) were much higher than the yield obtained from the use of pure methanol (36.6% yield). 28 …”

Section: Discussionmentioning

confidence: 99%

“…Many authors have reported excellent linear correlations between antioxidant/antiradical activity tests and total phenolic content. 31,28,32 Generally certain structural features in flavonoids are responsible for their high antioxidant activity; the presence of 2, 3-unsaturation on the C-ring and the number and substitution of hydroxyl groups on the A and B-rings. 32 The scavenging of the radical ABTS + by the extracts was found to be much lower than that of DPPH.…”

Section: Dpphmentioning

confidence: 99%

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In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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“…Çözücüler ekstrelerden rotary-evaporatör kullanılarak uçurulmuştur. Metanol ekstrelerinden elde edilen bileşiklerin daha yüksek biyoaktiviteye sahip olmaları nedeniyle çalışmalara metanol ekstresi ile devam edilmiştir [18].…”

Section: Bitkilerin Toplanması Ve Ekstre Edilmesiunclassified

Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

Yırtıcı¹

2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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Bu çalışmada Centaurea fenzlii Reichardt bitkisinin antioksidan özellikleri ve çeşitli enzimler üzerine inhibe edici etkisi araştırıldı. Bu amaçla çiçeklenme döneminde toplanan Centaurea fenzlii Reichardt'ın toprak üstü kısımları farklı polariteye sahip çözücülerde maserasyon yöntemi kullanılarak çözüldü ve ekstreleri elde edildi. Çalışmalara metanol ekstresi ile devam edildi. Metanol ekstresinin toplam fenolik ve flavonoid içerikleri sırasıyla, 16,72 mg GAE/g ka ve 173,16 mg KAE/g ka olarak belirlendi. Antioksidan kapasiteleri demir indirgeyici gücü (FRAP) için 0,256 mmol TE/g ka, bakır indirgeyici gücü (CUPRAC) için 0,878 mmol TE/g ka, ABTS için 0,354 mmol TE/g ka ve DPPH için 0,661 mmol TE/g ka olarak saptandı. Ayrıca, ekstrelerinin kolinesteraz, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı inhibe edici etkileri de belirlendi. Enzim inhibisyon etkisi sırasıyla, α-Glukozidaz için 0,331 mmol AKE/g ka, α-Amilaz için 0,354 mmol AKE/g ka, AChE için 0,367 mmol GAE/g ka, BChE için 0,878 mmol GAE/g ka ve Tirozinaz için mmol 0,256 KE/g ka olarak bulundu.

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Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers

Cited by 76 publications

References 35 publications

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

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