Phytochemical and antioxidant investigations of a Clausena anisata hook, a South African medicinal plant

Lawal, I. A.; Grierson, D. S.; Afolayan, A. J.

doi:10.4314/ajtcam.v12i1.5

Cited by 24 publications

(15 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Valencene (1.9%), para-cymene (0.3%), and a-cubebene (0.1%) were also present in the oil showing good correlation with the HO-MeOH components. Lawal et al (2015) who tested a South African H. odoratissimum L. Sweet species, found that a-humulene (5.6%) and b-caryophyllene (4.7%) were the major sesquiterpene compounds present.…”

Section: Gas Chromatography-mass Spectrometry and Liquid Chromatograpmentioning

confidence: 99%

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

et al. 2020

View full text Add to dashboard Cite

The Gram-positive bacterium Cutibacterium acnes (previously Propionibacterium acnes), plays an important role in the pathogenesis and progression of the dermatological skin disorder acne vulgaris. The methanolic extract of Helichrysum odoratissimum (L.) Sweet (HO-MeOH) was investigated for its ability to target bacterial growth and pathogenic virulence factors associated with acne progression. The gas chromatography-mass spectrometry (GC-MS) analysis of HO-MeOH identified a-humulene (3.94%), acurcumene (3.74%), and caryophyllene (8.12%) as major constituents, which correlated with previous reports of other Helichrysum species. The HO-MeOH extract exhibited potent antimicrobial activity against C. acnes (ATCC 6919) with a minimum inhibitory concentration (MIC) of 7.81 µg/ml. It enhanced the antimicrobial activity of benzoyl peroxide (BPO). The extract showed high specificity against C. acnes cell aggregation at sub-inhibitory concentrations, preventing biofilm formation. Mature C. acnes biofilms were disrupted at a sub-inhibitory concentration of 3.91 µg/ml. At 100 µg/ml, HO-MeOH reduced interleukin-1a (IL-1a) cytokine levels in C. acnes-induced human keratinocytes (HaCaT) by 11.08%, highlighting its potential as a comedolytic agent for the treatment of comedonal acne. The extract exhibited a 50% inhibitory concentration (IC 50 ) of 157.50 µg/ ml against lipase enzyme activity, an enzyme responsible for sebum degradation, ultimately causing inflammation. The extract's anti-inflammatory activity was tested against various targets associated with inflammatory activation by the bacterium. The extract inhibited pro-inflammatory cytokine levels of IL-8 by 48.31% when compared to C. acnes-induced HaCaT cells at 7.81 µg/ml. It exhibited cyclooxygenase-II (COX-II) enzyme inhibition with an IC 50 of 22.87 µg/ml. Intracellular nitric oxide (NO) was inhibited by 40.39% at 7.81 µg/ml when compared with NO production in lipopolysaccharide (LPS)induced RAW264.7 cells. The intracellular NO inhibition was potentially due to the 2.14 fold reduction of inducible nitric oxide synthase (iNOS) gene expression. The HO-MeOH extract exhibited an IC 50 of 145.45 µg/ml against virulent hyaluronidase enzyme activity, Frontiers in Pharmacology | www.frontiersin.org January 2020 | Volume 10 | Article 1559 1 which is responsible for hyaluronan degradation and scar formation. This study provides scientific validation for the traditional use of H. odoratissimum as an ointment for pimples, not only due to its ability to control C. acnes proliferation but also due to its inhibitory activity on various targets associated with bacterial virulence leading to acne progression.

show abstract

Section: Gas Chromatography-mass Spectrometry and Liquid Chromatograpmentioning

confidence: 99%

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The high concentrations of flavonoids, proanthocyanidin. flavonols, and phenols acts as the biological substances that inhibit diseases and thus serves as defence mechanism in humans; this includes antimicrobial, antioxidants and cytotoxicity activities ( Harborne and Williams, 2000;Lawal et al, 2015). These compounds are also very essential because of their ability to scavenge their hydroxyl groups, quench free radicals and decompose peroxides (Adedapo et al, 2008).…”

Section: Phytochemical Analysismentioning

confidence: 99%

Phytochemical constituents and antioxidant properties of acetone extract of <i>Cleome gynandra</i> (L.) growing in the Eastern Cape, South Africa.

Sowunmi¹,

Afolayan²

2015

Afr. J. Trad. Compl. Alt. Med.

Self Cite

View full text Add to dashboard Cite

Background: Several wild vegetables have been reported for their therapeutic benefits in South Africa. Many of these plants including Cleome gynandra (L.) lack scientific reports on its significance in folkloric medicine. Therefore, this study was undertaken to evaluate quantitatively the compositions of phytochemicals and antioxidant properties of acetone extract of different parts of C. gynandra. Materials and Method: Antioxidant activities were assessed against ferric reducing power, ABTS (2, 2'-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and NO (nitric oxide) radical scavenging activities. Total phenolics, flavonoids, flavanols, proanthocyanidins, tannins, saponins and alkaloids were also investigated. Results: Amongst the different plant parts, the leaf extract had the highest concentration of total phenolics (126.79 ± 0.55 mg/g), flavonoids (40.58 ± 0.06 mg/g) and flavanols (42.41 ± 0.05 mg/g) while the stem extract had the highest amount of proanthocyanidins (419.01 ± 0.67 mg/g) compared to the leaves (403.29 ± 0.89 mg/g) and fruits (107.18 ± 0.59 mg/g). The reducing power of the extracts was significantly higher than that of the standard drugs used in a concentration dependent manner. The activities of the plant extracts against ABTS, DPPH and NO radicals were dose responsive with IC 50 value of 0.2, 0.1 and 0.03 mg/g respectively. Conclusion: C. gynandra possesses high secondary metabolites which accounts for its strong antioxidant ability thus justifying its use as natural occurring antioxidants in folkloric medicine. The study encourages a regular consumption of this wild vegetable in order to avert oxidative stress related diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The results of the clustering analysis using the Darwin's 6 software showed that branching occurred at a very low phenon line, which suggests a broad to overall similarities among all landraces; this can be attributed to hybridization and ability of tomato to self-pollinate (Lawal et al, 2015). In this study, the first seven principal components (PC1, PC 2, PC3, PC4, PC5, PC6 and PC7) analysed covered 68.51% variation within the 14 dimensions generated (see online Supplementary Table S1).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity within African tomato using next generation sequencing

Mungai

Owino

Ambuko

et al. 2018

Plant Genet. Resour.

View full text Add to dashboard Cite

Full potential of African tomato has not been tapped due to lack of information regarding its characterization. The aim of this work was to study the diversity of 17 African tomato landraces collected from Solanaceae gene bank – Tanzania. Evaluation was done using Complete Random Block Design. Morphological data collected were subjected to GenStat's and Darwin6 software. RNA was extracted from leaf samples, fruits at three ripening stages using modified Trizol method and sequencing done using Illumina sequencing platform. The raw reads were filtered and analysed using the Bioinformatics tools. Phenotypically, the landraces clustered into three clusters dendrogram representation. Clustering was attributed by phenotypic variation. Analysis of variance showed significant phenotypic variations among the landraces (P < 0.05). A total of 115,965 validated single nucleotide polymorphisms (SNPs) were mined from the 303,754,051 high-quality filtered reads. Molecular characterization showed significant variation within the landraces at fruit development stages. Unlike the phenotypic variation, phylogenetic tree representation grouped the 17 landraces according to their geographical location with some landraces from different countries grouping together. The findings of this study reveal significant morphological variation among African tomato contributed by plant height, leaf blade length, leaf blade width and fruit width. Positive correlation between fruit width and yield (r = 0.93, P < 0.01) was observed. Results of this study reveal that there is admixture of landraces from various geographical locations. Morphological characterization of African tomato can only lay a foundation but it does not reveal genetic diversity. The transcriptome SNP analysis revealed significant variation among the African tomato according to their geographical location.

show abstract

Phytochemical and antioxidant investigations of a Clausena anisata hook, a South African medicinal plant

Cited by 24 publications

References 18 publications

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

Phytochemical constituents and antioxidant properties of acetone extract of <i>Cleome gynandra</i> (L.) growing in the Eastern Cape, South Africa.

Genetic diversity within African tomato using next generation sequencing

Contact Info

Product

Resources

About