Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry

Yang, Min; Sun, Jianghao; Lu, Zhenyu; Chen, Guangtong; Guan, Shu‐Hong; Liu, Xuan; Jiang, Baohong; Ye, Min; Guo, Dan

doi:10.1016/j.chroma.2008.08.097

Cited by 193 publications

(131 citation statements)

References 95 publications

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“…Metabolic study is crucial in clarifying how active constituents produce the effectiveness of the HM, yet HMs are complex and it is often difficult to determine which component is the active constituent. Furthermore, lacking standards is another challenge in assessing the biotransformation for the constituents in HMs (Huang et al, 2004;Yang et al, 2009;Jiang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Development of a Systematic Approach to Identify Metabolites for Herbal Homologs Based on Liquid Chromatography Hybrid Ion Trap Time-of-Flight Mass Spectrometry: Gender-Related Difference in Metabolism of SchisandraLignans in Rats

Liang

Hao²,

Xie³

et al. 2010

Drug Metab Dispos

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ABSTRACT:Metabolic research for herbal medicine (HM) is a formidable task, which is still in its infancy due to complicated components in HM, complex metabolic pathways, and lack of authentic standards. The present work contributes to the development of a powerful technical platform to rapidly identify and classify metabolites of herbal components based on a liquid chromatography hybrid ion trap time-of-flight mass spectrometry. Taking Schisandra lignans extract as an example, the metabolic studies were completed both in vitro and in vivo. In the in vitro study, metabolites for five representative Schisandra lignans were identified and structurally characterized. The major metabolic pathways were summed as demethylation, hydroxylation, and demethylation and hydroxylation. In the in vivo study, 44 metabolites were detected in rat urine. These metabolites were identified and classified rapidly according to the metabolic rules obtained in the in vitro studies, and hydroxylation was confirmed as the primacy metabolic pathway for lignans in rat urine. In addition, "relative cumulative excretion" (RCE) for the metabolites in female and male rats were calculated according to their relative intensities in the urine samples collected at 0 to 12, 12 to 24, and 24 to 36 h. As a result, great gender-related difference on RCE was observed. For most metabolites, RCE in female rats was significantly lower than that in male rats. In conclusion, the presently developed methodology and approach on metabolic research for Schisandra lignans will find its wide use in metabolic studies for herbal medicines.

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Section: Introductionmentioning

confidence: 99%

Development of a Systematic Approach to Identify Metabolites for Herbal Homologs Based on Liquid Chromatography Hybrid Ion Trap Time-of-Flight Mass Spectrometry: Gender-Related Difference in Metabolism of SchisandraLignans in Rats

Liang

Hao²,

Xie³

et al. 2010

Drug Metab Dispos

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show abstract

“…Besides, the hyphenations of LC-MS with other separation or analytical techniques have frequently been applied in natural products analysis. Several review articles dealing with LC-MS and its application in the analysis of natural products have been published [54][55][56][57][58][59]. With the aim of providing an up-to-date overview of LC-MS applications on the phytochemical analysis, the present review summarizes recent development of identification and measurement of phytochemical constituents and their metabolites, as well as fingerprint analysis for herbal medicines during the past few years (2007)(2008)(2009)(2010)(2011)(2012).…”

Section: Introductionmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

161

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“…For pharmaceutical preparation, the technique of HPLC-MS is still promising the characterization and isolation of chemical constituents from medicinal plants Yang et al, 2009). In addition to polysaccharides and flavanoids which have been commonly identified in many herbs, phytochemical analyses revealed 'marker components' that could be used for quality evaluation and standardization of specific herbs, for example, triterpenoids and -glucans in Ganoderma , cordycepin and ergosterol in Cordyceps (Paterson, 2008), ginsenoside in Ginseng ).…”

Section: Extraction and Fractionation Of Herbs -Recovery Of The Activmentioning

confidence: 99%

Traditional Chinese Herbal Medicine – East Meets West in Validation and Therapeutic Application

Yuen¹,

Tse²,

Yung³

2012

Recent Advances in Theories and Practice of Chinese Medicine

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Phytochemical analysis of traditional Chinese medicine using liquid chromatography coupled with mass spectrometry

Cited by 193 publications

References 95 publications

Development of a Systematic Approach to Identify Metabolites for Herbal Homologs Based on Liquid Chromatography Hybrid Ion Trap Time-of-Flight Mass Spectrometry: Gender-Related Difference in Metabolism of SchisandraLignans in Rats

Development of a Systematic Approach to Identify Metabolites for Herbal Homologs Based on Liquid Chromatography Hybrid Ion Trap Time-of-Flight Mass Spectrometry: Gender-Related Difference in Metabolism of SchisandraLignans in Rats

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Traditional Chinese Herbal Medicine – East Meets West in Validation and Therapeutic Application

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