Phytochemical analysis of selected Nepeta species by HPLC-ESI-MS/MS and GC–MS methods and exploring their antioxidant and antifungal potentials

Azizian, Tooba; Alirezalu, Abolfazl; Hassani, Abbas; Bahadori, S H Ranjbar; Sonboli, Ali

doi:10.1007/s11694-021-00819-8

Cited by 8 publications

(17 citation statements)

References 53 publications

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“…One of the most well-known species in the genus Nepeta is N. cataria . Several studies have performed qualitative identification of phytochemical constituents from leaves and flowers of N. cataria extract as well as oils from the plant (Edewor and Usman, 2011 ; Reichert et al, 2018 ; Azizian et al, 2021 ). The antibacterial of N. cataria from previous research likewise demonstrated sufficient antibacterial activity against S. aureus, K. pneumoniae and S. typhi (Mukhtar and Singh, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Nadeem

Shahzad²,

Ahmed³

et al. 2022

Front. Plant Sci.

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Traditional and phytochemical studies have confirmed the richness and diversity of medicinal plants such as Nepeta cataria (N. cataria), but more studies are needed to complete its metabolite profiling. The objective of this research was to enhance the metabolomic picture and bioactivity of N. cataria for better evaluation. Phytochemical analysis was performed by bio-guided protocols and gas chromatography-mass spectrometry (GC/MS). For this, solvents such as methanol, ethanol, water, acetone, and hexane were used to extract a wide number of chemicals. Antibacterial analysis was performed using the 96-well plate test, Kirby Bauer's disk diffusion method, and the resazurin microdilution test. Antioxidant activity was determined by the DPPH assay and radical scavenging capacity was evaluated by the oxygen radical absorbance capacity (ORAC) assay. GC/MS analysis revealed a total of 247 identified and 127 novel metabolites from all extracts of N. cataria. Water and acetone extracts had the highest identified metabolites (n = 79), whereas methanol extract was the highest in unidentified metabolites (n = 48). The most abundant phytochemicals in methanol extract were 1-isopropylcyclohex-1-ene (concentration = 27.376) and bicyclo [2.2.1] heptan-2-one (concentration = 20.437), whereas in ethanol extract, it was 9,12,15-octadecatrienoic acid (concentration = 27.308) and 1-isopropylcyclohex-1-ene (concentration = 25.854). An abundance of 2 methyl indoles, conhydrin, and coumarin was found in water extracts; a good concentration of eucalyptol was found in acetone extract; and 7,9-di-tert-butyl-1-oxaspiro is the most abundant phytochemicals in hexane extracts. The highest concentration of flavonoids and phenols were identified in hexane and methanol extracts, respectively. The highest antioxidant potential (DPPH assay) was observed in acetone extract. The ethanolic extract exhibited a two-fold higher ORAC than the methanol extract. This examination demonstrated the inhibitory effect against a set of microbes and the presence of polar and non-polar constituents of N. cataria. The results of this study provide a safe resource for the development of food, agriculture, pharmaceutical, and other industrial products upon further research validation.

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Section: Discussionmentioning

confidence: 99%

“…The results from our studies corroborate the results exhibited in previous studies. In addition to N. cataria , other species of the Nepeta genus have also been studied extensively for their phytochemical analysis, and among all species, N. cataria is the most promising of all species (Azizian et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Nadeem

Shahzad²,

Ahmed³

et al. 2022

Front. Plant Sci.

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“…Different species of the genus Nepeta are used due to their antiasthmatic, antitussive, antioxidant (Azizian et al, 2021), diuretic, antipyretic, expectorant (Kordali et al, 2015, antispasmodics (Rather et al, 2012), insecticidal, acaricidal (Çalmaşur et al, 2006), antifungal (Sonboli et al, 2004 and cytostatic properties (Al-Oqail et al, 2014), among others.…”

Section: Introductionmentioning

confidence: 99%

Influence of Foliar Fertilisers on Biochemical and Physiological Properties in Nepeta Racemosa Lam.

Lungoci¹,

JITAREANU²,

Ghiţău³

et al. 2022

JALSE

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The purpose of this study was to evaluate some elements of physiology and biochemistry in Nepeta racemosa Lam. in the pedo-climatic conditions of the Moldavian forest steppe. The parameters were quantified under conditions of differentiated fertilisation with three foliar products (KSC 1, KSC 2, and Corona K). Biochemical analysis by GC/MS revealed the highest concentration of the following components: nepetallic acid (Untreat - 54.33%, KSC 1 - 55.50%, KSC 2 - 56.90%, Corona K - 53.77), eucalyptol (Untreat . 9.87%, KSC 1 - 10.70%, KSC 2- 12.80%, Corona K - 10.78%), 3-hydroxy-(4S, 4aS, 7S, 7aS)-dihydronepetalactone (Untreat - 5.87%, KSC 1 - 5.37%, KSC 2 - 4.87, Corona K - 3.67%), and (4aS, 7S, 7aS)-trans, cis-nepetalactone (Untreat - 4.30%, KSC 1 - 5.00%, KSC 2 - 4.20%, Corona K - 5.23%). Polyphenols were present in large quantities in the Corona K variant, with 33.1 mg GA/g dw at the first harvest and 27.88 mg GA/g dw at the second harvest. The highest concentration of flavonoids was identified in the Corona K variant (26.34 mg QE/g dw - first harvest and 56.77 mg QE/g dw - second harvest). The antioxidant activity was between 14.76 and 38.54% at the first harvest, and from 12.18 to 22.95% at the second harvest. Chlorophyll pigments ranged from 13.12-17.68 μg/ml-1 for chlorophyll a, 5.43-9.66 μg/ml-1 for chlorophyll b, and 2.07-3.34 μg/ml-1 for carotenoids. The highest grass production per m2 was achieved in the version fertilised with KSC 1 for both crops, with 986.66 g/m2 for the first harvest and 861.33 g/m2 for the second. Iridoids are a class of substances that are commonly used for pesticide role. The study aims to highlight these substances and determine whether differentiated fertilization influences. The presented data confirm the results in the literature for polyphenols and flavonoids, but also present chemotypic novelties in terms of chemical composition and represent a direction to follow for future research.

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“…Numerous phytochemical studies with the genus have shown that the main constituents of Nepeta species are nepetalactones, iridoids, and their glycosides as well as diterpenes, triterpenes, and flavonoids. Moreover, most Nepeta species are rich in essential oils [ 9 , 10 , 11 ]. In parallel with their rich chemical content, including medicinally important compounds, and traditional uses, plants belonging to the genus have been shown to have many important biological activities.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Some Nepeta Species against Breast Cancer Cell Lines and Their Associated Phytochemical Properties

Şafak

Karatoprak

Dırmencı

et al. 2022

Plants

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Nepeta is one of the largest genera of the Lamiaceae family. Nepeta species are commonly employed in traditional medicine for a variety of ailments, as well as food additives. In addition, they also come to the fore with their rich phytochemical content. In the present study, the quantitative phytochemical content of methanolic extracts and infusions prepared from the aerial parts of 14 Nepeta taxa collected from Turkey and their cytotoxic effects on two breast cancer cell lines, MCF-7 and MDA-MB-231, were investigated by using the MTT (3-(4,5-dimethylthiazol-2-yl))-2,5-diphenyltetrazolium-bromide) test. According to HPLC-PDA analysis, N. racemosa methanolic extract had the highest ursolic acid content with 165.9 mg/g extract. Total sterol, total iridoid, and total triterpenoid content were determined to be greatest in the methanolic extracts of N. meyeri, N. trichocalyx and N. phyllochlamys. The MTT experiment demonstrated that certain Nepeta species suppressed the growth of MCF-7 and MDA-MB-231 cells in a dose-dependent manner. Statistical analysis revealed a strong correlation between the cytotoxic effects of the extracts and their triterpene content. In conclusion, the data obtained from this study are important in terms of forming a basis for advanced anticancer activity studies on breast cancer with Nepeta sp.

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Phytochemical analysis of selected Nepeta species by HPLC-ESI-MS/MS and GC–MS methods and exploring their antioxidant and antifungal potentials

Cited by 8 publications

References 53 publications

Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Influence of Foliar Fertilisers on Biochemical and Physiological Properties in Nepeta Racemosa Lam.

Cytotoxic Effects of Some Nepeta Species against Breast Cancer Cell Lines and Their Associated Phytochemical Properties

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