Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts of Carduus macracanthus

Anza, Mathewos; Bibiso, Mesfin; Alemayehu, Bereket; Desalegn, Engeda

doi:10.12980/jclm.5.2017j7-110

Cited by 2 publications

(2 citation statements)

References 10 publications

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“… 43 Another study from Ethiopia showed that acetone extract of P. abyssinica had antimicrobial activities against S. aureus, E. coli , S. Typhi, and B. cereus . 44 Another active plant found in our study was R. nepalensis . The EtOH extract of this plant entirely prevented the growth of ATCC, and the second most effective was the EtOAc extract.…”

Section: Discussionmentioning

confidence: 58%

Antibacterial Activity Against Multidrug-Resistant Clinical Isolates of Nine Plants from Chencha, Southern Ethiopia

Manilal¹,

Sabu²,

Abebe³

et al. 2023

IDR

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Background:The diminishing efficacy of antibiotics currently in use and the emergence of multidrug-resistant bacteria pose a grave threat to public health worldwide. Hence, new classes of antimicrobials are urgently required, and the search is continuing. Methods: Nine plants were chosen for the current work, which are collected from the highlands of Chencha, Ethiopia. Plant extracts containing secondary metabolites in various organic solvents were checked for antibacterial activity against type culture bacterial pathogens and MDR clinical isolates. The broth dilution technique was used to evaluate the minimum inhibitory and minimum bactericidal concentrations of highly active plant extracts, and time-kill kinetic and cytotoxic assays were performed using the most active plant extract. Results: Two plants (C. asiatica and S. marianum) were highly active against ATCC isolates. The EtOAc extract of C. asiatica produced the highest zone of inhibition ranging between 18.2±0.8-20.7±0.7 and 16.1±0.4-19.2±1.4 mm against Gram-positive and Gram-negative bacteria, respectively. The EtOH extract of S. marianum displayed zones of inhibition in the range of 19.9±1.4-20.5 ±0.7 mm against the type culture bacteria. The EtOAc extract of C. asiatica effectively curbed the growth of six MDR clinical isolates. The MIC values of C. asiatica against the Gram-negative bacteria tested were 2.5 mg/mL, whereas the corresponding MBC values were 5 mg/mL in each case. The MIC and MBC values were the lowest in the case of Gram-positive bacteria, ie, 0.65 and 1.25 mg/ mL, respectively. A time-kill assay showed the inhibition of MRSA at 4 × MIC and 8 × MIC within 2 hours of incubation. The 24 h LD 50 values of C. asiatica and S. marianum corresponding to Artemia salina were 3.05 and 2.75 mg/mL, respectively. Conclusion: Overall results substantiate the inclusion of C. asiatica and S. marianum as antibacterial agents in traditional medicines.

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Section: Discussionmentioning

confidence: 58%

Antibacterial Activity Against Multidrug-Resistant Clinical Isolates of Nine Plants from Chencha, Southern Ethiopia

Manilal¹,

Sabu²,

Abebe³

et al. 2023

IDR

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“…The root extract at 100 and 200 mg/ml did not have any effect on Gram-negative bacteria ( P. aeruginosa , E. coli , and K. pneumonia ) as it was evident by an equal level of zone inhibition with the negative control. However, in other studies, the root extract of C. macracanthus suppressed the growth E. coli at a concentration of 50 mg/mL [ 24 ]. Notably, different studies have reported varying effects of C. leptacanthus extracts on bacteria, which may be attributed to differences in their constituents.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of a Phenylpropanoid from the Root Extract of Carduus leptacanthus Fresen

Dagne,

Degu,

Abebe

et al. 2023

Journal of Tropical Medicine

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Background. The emergence and rapid spread of antimicrobial drug-resistance microorganisms exacerbate the treatment of infectious diseases, underscoring the importance of finding new, safe, and effective drugs. In Ethiopia, the roots of Carduus leptacanthus have traditionally been employed to treat microbial infectious diseases The aim of this study was to evaluate the antibacterial activity of the root extract and its primary components against six bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia). Methods. The extraction involved maceration of air-dried and powdered roots of C. leptacanthus with 80% methanol. The compound was isolated from the root extract using silica gel column chromatography and recrystallization in CHCl3/MeOH (9 : 1) and was characterized using ESI-MS and 1D-NMR spectroscopy. Antibacterial activity of the extract was assessed using agar well diffusion and broth microdilution methods. Results. Syringin, a phenylpropanoid, was isolated and characterized from the extract of C. leptacanthus. The extract showed the most substantial efficacy against S. epidermidis (MIC = 5.33 mg/ml and inhibition zone diameter of 24 mm at 200 mg/m). Syringin also elicited antibacterial activity against S. aureus (MIC = 13.33 mg/ml), S. epidermidis (MIC = 16 mg/ml), and S. agalactiae (MIC = 16 mg/ml). Despite being tested up to a maximum concentration of 16 mg/ml, syringin did not exhibit antibacterial activity against the Gram-negative bacteria (P. aeruginosa, E. coli, and K. pneumonia). Conclusions. In conclusion, the findings suggest that syringin exhibits partial involvement in the root extract’s antibacterial activity, thereby potentially supporting the traditional medicinal use of the plant.

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Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts of Carduus macracanthus

Cited by 2 publications

References 10 publications

Antibacterial Activity Against Multidrug-Resistant Clinical Isolates of Nine Plants from Chencha, Southern Ethiopia

Antibacterial Activity Against Multidrug-Resistant Clinical Isolates of Nine Plants from Chencha, Southern Ethiopia

Antibacterial Activity of a Phenylpropanoid from the Root Extract of Carduus leptacanthus Fresen

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