Phytochemical Analysis, Estimation of Quercetin, and in Vitro Anti-Diabetic Potential of Stevia Leaves Samples Procured from Two Geographical Origins

Das, Kuntal; Asdaq, Syed Mohammed Basheeruddin; Khan, Marwa; Singirikonda, Sravani; Alamri, Abdulhakeem S.; Alsanie, Walaa F.; Alhomrani, Majid; Sreeharsha, Nagaraja; Venugopala, Katharigatta N.

doi:10.32604/phyton.2022.022379

Cited by 1 publication

(1 citation statement)

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“…However, the contribution of quercetin 6-C-glucoside to the antihyperglycemic properties of HR could not be excluded, since it was selected as the second powerful inhibitor of αglucosidase. In this context, flavonol glycosides with 2,3-double bond and ketone group at C4, as well as free OH group at C3 in the ring C have been reported as the most effective α-glucosidase inhibitors [51,52]. Even that quercetin 6-C-glucoside possesses these structural features, its glucose moiety may cause steric hindrance and limitation of binding interaction within the α-glucosidase active center due to increased molecular size and polarity [53].…”

Section: Antidiabetic Activitymentioning

confidence: 99%

In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures

Tusevski¹,

Todorovska²,

Станоева³

et al. 2023

Phyton

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Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed to the production of oxygenated and prenylated xanthones. The neuroprotective potential of HR extracts was related to the accumulation of quercetin 6-C-glucoside, epicatechin, procyanidins and γ-mangostin isomers as potential inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Vanillic acid and prenylated xanthones in HR clones as promising inhibitors of tyrosinase additionally contributed to the neuroprotective activity. Five preeminent xanthones in HR (γ-mangostin, mangiferin, garcinone C, garcinone E and 1,3,7-trihydroxy-6-metoxy-8-prenyl xanthone) along with the flavonol quercetin 6-C-glucoside effectively inhibited α-amylase and α-glucosidase indicating the antidiabetic properties of HR extracts. Transgenic roots of H. perforatum can be exploited for the preparation of novel phytoproducts with multi-biological activities.

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Section: Antidiabetic Activitymentioning

confidence: 99%