Phytochemical Analysis, Antibacterial Activity, FTIR and GCMS Analysis of Ceropegia juncea Roxb.

Visveshwari, M; Subbaiyan, B; Thangapandian, V.

doi:10.25258/phyto.v9i07.11155

Cited by 7 publications

(2 citation statements)

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“…The crude extract (10 mg) was dissolved in 5 ml of the respective solvent to make a stock solution. From the stock solution 1 ml of solution was taken and diluted by adding 9 ml of the respective solvent following the methodologies of Adinew (2014) and Visveshwari et al (2017). The samples were then exposed to GC–MS analysis.…”

Section: Methodsmentioning

confidence: 99%

Chromatographic analysis and antioxidant potency of the crude extract of Xanthium spinosum in various fractions

Barkatullah,

Shabana,

Nafees

2023

Biomedical Chromatography

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Pharmacology experts place a high priority on therapeutic plants because the majority of pharmaceutical firms rely on medicinal plants as raw ingredients. Therefore, the potential bioactive components using gas chromatography–mass spectrometry analysis and antioxidant effects using DPPH free radical scavenging activity of various crude fractions of Xanthium spinosum were assessed. Gas chromatography–mass spectrometry analysis showed the presence of various bioactive compounds including benzenedicarboxylic acid (18.60%), 8‐octadecenoic acid (4.86%), 11‐octadecenoic acid and 10‐octadecenoic acid in the crude methanolic extract, 1,2‐benzenedicarboxylic acid, diisooctyl ester (14.42%), 1,2‐benzenedicarboxylic acid, mono (2‐ethylhexyl) ester (14.42%), 6‐octadecenoic acid, methyl ester (7.56%), 8‐octadecenoic acid, methyl ester (7.56%), 10‐octadecenoic acid, methyl ester (7.56%) and hexadecanoic acid, methyl ester (6.55%) in the n‐hexane extract, ethanal, 2‐methyl‐2‐[4‐(1‐methylethyl)phenyl]‐(3.02%), (+)‐3‐carene, 4‐isopropenyl‐(3.02%), 7H‐indeno[5,6‐b] furan‐7‐one, 4,4a,5,6,7a,8‐hexahydro‐ (3.02%) and 2‐[5‐(2,2‐dimethyl‐6‐methylene‐cyclohexyl)‐3‐methyl‐pent‐2‐enyl]‐[1,4] benzoquinone (2.79%) in the chloroform extract and 1,2‐benzenedicarboxylic acid, mono (2‐ethylhexyl) ester (33.005%), 1,2‐benzenedicarboxylic acid, diisooctyl ester (33.005%) and bis(2‐ethylhexyl) phthalate (33.005%) in the ethyl acetate extract. Significant DPPH radical scavenging activity was exhibited by the chloroform fraction (43.37–88.65%) at all doses followed by the crude methanolic extract (36.02–83.75%) at all doses. In conclusion, different crude fractions of X. spinosum can be considered a rich source of pharmacologically active components that can be scoped for isolation and may be subjected to in‐depth pharmacological study.

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Section: Methodsmentioning

confidence: 99%

Chromatographic analysis and antioxidant potency of the crude extract of Xanthium spinosum in various fractions

Barkatullah,

Shabana,

Nafees

2023

Biomedical Chromatography

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“…Molecular phylogenetic analysis of 81 Ceropegias aligned them in many clades (Surveswaran et al., 2009). The methanol extract of C. juncea are medicinally valuable and possess various pharmaceutical applications (Visveshwari et al., 2017). It can be concluded that insufficient study has been done so far on C. anjanerica, As the species has ornamental and medicinal potential, conservational actions should be undertaken.…”

Section: Threat and Conservation Statusmentioning

confidence: 99%

Occurrence and Conservation Status of Ceropegia anjanerica Malpure et al., in Nasik District

Dinkar,

Prabhakar,

Prabhakar

et al. 2023

IJPE

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The present review article deals with the distribution, morphology, habitat, ecology, threat, and conservation status of Ceropegia anjanerica Malpure et al., in and around Nasik district. The genus Ceropegia L. belongs to the subfamily Asclepiadoideae (milk weeds) within thefamily Apocynaceae. A total of 6 species and 2 varieties of this genus have been recorded in Nasik district viz., C. anjanerica Malpure; Kamble and Yadav, C. bulbosa Roxb. var. bulbosa, C. bulbosa Roxb. var. lushii (Graham) Hook.f., C. hirsuta Wight and Arn., C. lawii Hook.f.,C. mahabalei Hemadri and Ansari, C. media (H. Huber) Ansari and C. vincifolia Hook. Of these species, C. anjanerica is the ‘endangered’ plant species endemic to Anjaneri hills of Nasik district part of the northern western Ghats. The plant body is erect herbaceous withtuberous rootstock, tubers are generally 2-5 cm in diameter, globose or discoid with fibrous roots. Recent investigations show that this species has been recorded from hill tops, plateaus, and adjacent village areas.

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Inhibition of HIV‐1 Protease by Carpobrotus edulis (L.)

Omoruyi

Ighodaro

Afolayan

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Carpobrotus edulis (L.) is a plant commonly found in the Eastern Cape Province of South Africa and is used for the general treatment of infections relating to the human immunodeficiency virus (HIV). HIV-1 protease plays an important role during HIV replication and maturation to its infectious form, and therefore inhibition of the enzyme is one of the main focus areas in drug development. The inhibitory effect of a water extract of C. edulis leaves against HIV-1 protease activity was determined using the SensoLyte® 520 HIV-1 protease assay fluorimetric kit and employing a HiLyte Fluor™488/QXL™520 fluorescence resonance energy transfer (FRET) peptide. Cytotoxicity of the extract towards HeLa Chang cell lines was determined using an in vitro MTT assay, and the phytochemical profile of the extract was determined with FT-IR and LC-MS. HIV-1 protease activity was inhibited 83.06% (IC50 1.6 mg/ml) (p<0.0001) by the pepstatin A inhibitor control. Treatment with all C. edulis extract concentrations (16, 1.6, 0.16, and 0.016 mg/ml) inhibited HIV-1 protease activity significantly (p<0.0001) in a typical dose response manner. With regards to cytotoxicity, the negative controls containing untreated HeLa Chang cells exhibited high formazan formation rates in contrast with the positive controls, containing curcumin, which reduced formazan formation significantly (p<0.001), exhibiting cytotoxicity towards the cells. There was no significant (p>0.05) difference in the formazan formation rates between the negative controls and 1, 0.5, 0.125, 0.065, 0.031, and 0.015 mg/ml plant extract, confirming no toxicity of C. edulis extracts towards HeLa Chang cells. Major functional phytochemical compounds identified included alcohols, phenols, alkanes, amines, carboxylic acids, and esters. LC-ESI-TOF/MS analysis revealed the putative identities of main compounds present in the aqueous leaves extract, including some that contribute to its anti-HIV-1 protease action.

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Phytochemical Analysis, Antibacterial Activity, FTIR and GCMS Analysis of Ceropegia juncea Roxb.

Cited by 7 publications

References 0 publications

Chromatographic analysis and antioxidant potency of the crude extract of Xanthium spinosum in various fractions

Chromatographic analysis and antioxidant potency of the crude extract of Xanthium spinosum in various fractions

Occurrence and Conservation Status of Ceropegia anjanerica Malpure et al., in Nasik District

Inhibition of HIV‐1 Protease by Carpobrotus edulis (L.)

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