Phytochemical analysis and broad spectrum antimicrobial activity of ethanolic extract of Jasminum mesnyi Hance leaves and its solvent partitioned fractions

Abstract

Jasminum mesnyi Hance (yellow jasmine, Family Oleaceae) belongs to an important subclass of Jasminum family whose biological significance is under explored. The current study pertains to isolation of various solvent extracts fractions and their anti-bacterial effects on the Gram-positive and Gram-negative bacterial pathogens. Ethanolic extract of J. mesnyi Hance leaves was subjected to further partitioning using different solvents with increasing polarity to get different solvent fractions. Different extracts were analysed for their phytochemical constitutents and were evaluated for their antibacterial activity against a number of diseases causing bacteria. Diethylether fraction (DEF) showed remarkable inhibition of growth of Aeromonas hydrophila and Vibrio parahaemolyticus with inhibition zones of 17 and 19mm, respectively at 250 μg. On the other hand, the hexane fraction (HF) was found to be more effective against Gram- positive test bacterial pathogens (Bacillus anthracis and Bacillus subtilis) with inhibition zones of 19.6 mm and 17.5 mm, respectively. The phytochemical screening of different test fractions revealed the predominant presence of cardiac glycosides, sterols and terpenoids in DEF and HF, while Ethylacetate fraction (EAF) and methanol fractions (MF) were found rich in flavonoids and phenols with moderate amount of other reference metabolites.

Traditional Uses of Medicinal Plants by Ethnic People in the Kavrepalanchok District, Central Nepal

“…Jasminum mesnyi ( Figure 4 C), commonly known as Japanese jasmine, has been reported in our survey to cure fever and typhoid. The antimicrobial potential of the leaf extract of this species has been recently demonstrated on Gram-positive and Gram-negative bacterial pathogens [ 71 ].…”

Rosmarinic acid attenuates inflammation in experimentally induced arthritis in Wistar rats, using Freund’s complete adjuvant

“…Fractions 1‐25 were eluted with chloroform: methanol (95:5), fractions 28‐40 were eluted with chloroform: methanol (90:10), fractions 42‐85 were eluted with chloroform: methanol (80:20), fractions 90‐135 were eluted with chloroform: methanol (75:25), and fractions 140‐165 were eluted with chloroform: methanol (50:50). All the fractions were screened for the presence of various active phytocompounds . All the collected fractions (F 1 ‐F 5 ) were evaluated for in vitro anti‐arthritic activity by inhibition of protein denaturation method (protocol not shown here).…”

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“…The various secondary metabolites namely alkaloids, glycosides, flavonoids, steroids, terpenoids, tannins and saponins are substantiated in different parts of the plants 5,10,11 . The pharmacological studies proved their efficacy in J. multiflorum towards antioxidant 12,13 , antimicrobial 14 , antihelmintic 15 , nemacitidal 16 , GI 17 , in cardiovascular diseases 18,19 and effect on central nervous system 20,21 and J. mesnyi revealed its wound healing potential of roots 22 , antioxidant 23,24 , antiulcer 25 , antihyperglycemic 26 , antihelmintic 27 and antimicrobial 28 efficacy of leaves were substantiated. The main hindrance to the acceptance of herbal medicines is the lack of documentation and quality control.…”