Phytochelatin–metal(loid) transport into vacuoles shows different substrate preferences in barley and <i><scp>A</scp>rabidopsis</i>

Song, Won-Yong; Mendoza‐Cózatl, David G.; Lee, Youngsook; Schroeder, Julian I.; Ahn, Sang-Nag; Lee, Hyun‐Sook; Wicker, Thomas; Martinoia, Enrico

doi:10.1111/pce.12227

Cited by 139 publications

(73 citation statements)

References 39 publications

(102 reference statements)

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“…These findings indicate that AtABCC1 and AtABCC2 play a major role in As detoxification. Recently, vacuoles isolated from barley were shown to have a pattern of PC2-As transport similar to that of Arabidopsis vacuoles (24), suggesting that similar ABC transporters are involved in vacuolar sequestration in monocotyledonous plants such as barley and rice.…”

Section: Significancementioning

confidence: 99%

A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

Song

Yamaki

Yamaji

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.A rsenic (As) is a highly toxic metalloid that is classified as a nonthreshold class-1 carcinogen (1, 2). Long-term exposure to As in humans causes a number of diseases, including hyperpigmentation, keratosis, and skin and internal cancers (3). Due to As contamination of drinking water and soil from both anthropogenic and geogenic sources, millions of people worldwide suffer from As toxicity. This problem is particularly serious in countries in South and Southeast Asia, such as India and Bangladesh, where groundwater, which is used both as a drinking water supply and for irrigating rice, contains high concentrations of As (4). Therefore, reducing the As concentration in drinking water and foods is a critical goal for promoting human health.Rice (Oryza sativa L.), a staple food of half of the world's human population, is a major dietary source of As (5, 6). A recent cohort study in West Bengal, India showed that high concentrations of As in rice are associated with elevated genotoxic effects in humans (7). Rice accumulates As in the shoots and grains more efficiently than do other cereal crops such as wheat (Triticum aestivum) and barley (Hordeum vulgare) (8, 9). This higher efficiency has been attributed to the increased bioavailability of As under flooded conditions (such as those found in paddy fields) and the efficient As uptake system in rice (10-12). In the anaerobic paddy field, As is mainly present in the form of arsenite, which is taken up by two silicon (Si) transporters-namely, Lsi1 (low silicon 1), a Si influx transporter, and Lsi2 (low silicon 2), a Si efflux transporter (11). These transpo...

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Section: Significancementioning

confidence: 99%

A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

Song

Yamaki

Yamaji

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

389

241

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“…In rice, OsHMA3 is localized to the root tonoplast and sequesters Cd in the vacuoles of root cells (Ueno et al, 2010). Phytochelatin synthase (PCS) assembles the heavy metal-phytochelatin complexes, and concerted action of both PCS and an ATPase-type transporter appears to be required for vacuolar transport of Cd in barley (Hordeum vulgare L., Song et al, 2014). Phytochelatin synthase (PCS) assembles the heavy metal-phytochelatin complexes, and concerted action of both PCS and an ATPase-type transporter appears to be required for vacuolar transport of Cd in barley (Hordeum vulgare L., Song et al, 2014).…”

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confidence: 99%

Prospects for Selecting Wheat with Increased Zinc and Decreased Cadmium Concentration in Grain

et al. 2015

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Wheat (Triticum aestivum L.) is a primary staple cereal and significant source of mineral nutrients in human diets. Therefore, increasing concentration of the essential mineral, Zn, and decreasing concentration of the toxic mineral, Cd, could significantly improve human health. Because plant mechanisms for uptake and translocation of Cd and Zn are related, we assessed both Cd and Zn concentration to evaluate their independence in hard winter wheat germplasm. Grain Cd concentrations of some genotypes grown in Nebraska trials were above the Codex guidance level (0.2 mg kg–1), and highly repeatable differences in grain Cd were found between pairs of low and moderate‐Cd commercial cultivars. Grain Cd concentration was predicted by Cd concentration in aboveground plant tissues at anthesis. However, grain Zn concentration was not predicted by Zn concentration in aboveground plant tissues. Genome‐wide association scans using high‐density single nucleotide polymorphism (SNP) markers identified Cd‐associated SNPs on 5AL in a region homoeologous to the Cdu1 locus on 5BL in durum wheat (Triticum turgidum L. var. durum Desf.). Genetic regulation of grain cadmium concentration in bread wheat may be more complex than in durum wheat because epistatic interactions between SNP markers were identified, and SNP marker haplotypes were imperfect predictors of grain Cd phenotype. The SNP marker associations with Zn concentration were weak and inconsistent across trials, and Zn concentration was independent of 5AL markers. The independent genetic regulation of grain Cd and Zn concentrations indicates that breeding low Cd hard winter wheat genotypes without reducing Zn concentration has high potential for success.

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“…16,17,18 The primary detoxification strategy against heavy metals employ glutathione (GSH) and phytochelatins (PC) in different plant species such as Arabidopsis, Brassica, Zea mays and rice. [19][20][21][22][23][24] Apart from heavy metals, the role of GSH has also been well documented in providing resistance against other abiotic stresses. 25,26 These processes require efficient sulfur uptake and homeostasis in plants which is dependent on multigene family encoding sulfate transporters.…”

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confidence: 99%