Phytochelatin-mediated metal detoxification pathway is crucial for an organomercurial phenylmercury tolerance in Arabidopsis

Uraguchi, Shimpei; Ohshiro, Yuka; Otsuka, Yuto; Wada, Eitaro; Naruse, Fumii; Sugaya, Kakeru; Nagai, Kazuo; Wongkaew, Arunee; Nakamura, Ryosuke; Takanezawa, Yasukazu; Clemens, Stephan; Ohkama‐Ohtsu, Naoko; Kiyono, Masako

doi:10.1007/s11103-021-01221-0

Cited by 11 publications

(10 citation statements)

References 53 publications

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“…Phytochelatins (PCs) are a type of cysteine-rich peptide [ 50 ]. Heavy metal pollutants, such as copper, arsenic, cadmium, and zinc, can induce the synthesis of PCs [ 51 , 52 ] in plants that can act as detoxifying agents by chelating heavy metals with their thiol groups. Previous studies have shown that plant non-protein thiols (NPTs) are composed mainly of PCs, so they can be measured to indirectly reflect PC content [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Reduction of Arsenic Uptake and Alleviation of Leaf Arsenic Toxicity in Maize (Zea mays L.) by Arbuscular Mycorrhizal Fungi (AMF) and Exogenous Iron through Antioxidant Activity

Zhou

Fuzhao

Chen

et al. 2023

JoF

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Arbuscular mycorrhizal fungi (AMF) play key roles in enhancing plant tolerance to heavy metals, and iron (Fe) compounds can reduce the bioavailability of arsenic (As) in soil, thereby alleviating As toxicity. However, there have been limited studies of the synergistic antioxidant mechanisms of AMF (Funneliformis mosseae) and Fe compounds in the alleviation of As toxicity on leaves of maize (Zea mays L.) with low and moderate As contamination. In this study, a pot experiment was conducted with different concentrations of As (0, 25, 50 mgꞏkg−1) and Fe (0, 50 mgꞏkg−1) and AMF treatments. Results showed that under low and moderate As concentrations (As25 and As50), the co-inoculation of AMF and Fe compound significantly increased the biomass of maize stems and roots, phosphorus (P) concentration, and P-to-As uptake ratio. Moreover, the co-inoculation of AMF and Fe compound addition significantly reduced the As concentration in stem and root, malondialdehyde (MDA) content in leaf, and soluble protein and non-protein thiol (NPT) contents in leaf of maize under As25 and As50 treatments. In addition, co-inoculation with AMF and Fe compound addition significantly increased the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) in the leaves of maize under As25 treatment. Correlation analysis showed that stem biomass and leaf MDA content were very significantly negatively correlated with stem As content, respectively. In conclusion, the results indicated that the co-inoculation of AMF and Fe compound addition can inhibit As uptake and promote P uptake by maize under low and moderate As contamination, thereby mitigating the lipid peroxidation on maize leaves and reducing As toxicity by enhancing the activities of antioxidant enzymes under low As contamination. These findings provide a theoretical basis for the application of AMF and Fe compounds in the restoration of cropland soil contaminated with low and moderate As.

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Section: Discussionmentioning

confidence: 99%

Synergistic Reduction of Arsenic Uptake and Alleviation of Leaf Arsenic Toxicity in Maize (Zea mays L.) by Arbuscular Mycorrhizal Fungi (AMF) and Exogenous Iron through Antioxidant Activity

Zhou

Fuzhao

Chen

et al. 2023

JoF

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“…Chelating reactions are a key component for detoxification of metals in organic matter. Phytochelatins and phytochelatin synthases are essential players in the bioremediation process for plant or algae metal processing (Balzano et al, 2020;Uraguchi et al, 2022). These chelatins serve as small metal-binding ligand peptides.…”

Section: Microalgae For Heavy Metal Removalmentioning

confidence: 99%

A synthetic biology approach for the treatment of pollutants with microalgae

Webster,

Villa-Gomez,

Brown

et al. 2024

Front. Bioeng. Biotechnol.

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The increase in global population and industrial development has led to a significant release of organic and inorganic pollutants into water streams, threatening human health and ecosystems. Microalgae, encompassing eukaryotic protists and prokaryotic cyanobacteria, have emerged as a sustainable and cost-effective solution for removing these pollutants and mitigating carbon emissions. Various microalgae species, such as C. vulgaris, P. tricornutum, N. oceanica, A. platensis, and C. reinhardtii, have demonstrated their ability to eliminate heavy metals, salinity, plastics, and pesticides. Synthetic biology holds the potential to enhance microalgae-based technologies by broadening the scope of treatment targets and improving pollutant removal rates. This review provides an overview of the recent advances in the synthetic biology of microalgae, focusing on genetic engineering tools to facilitate the removal of inorganic (heavy metals and salinity) and organic (pesticides and plastics) compounds. The development of these tools is crucial for enhancing pollutant removal mechanisms through gene expression manipulation, DNA introduction into cells, and the generation of mutants with altered phenotypes. Additionally, the review discusses the principles of synthetic biology tools, emphasizing the significance of genetic engineering in targeting specific metabolic pathways and creating phenotypic changes. It also explores the use of precise engineering tools, such as CRISPR/Cas9 and TALENs, to adapt genetic engineering to various microalgae species. The review concludes that there is much potential for synthetic biology based approaches for pollutant removal using microalgae, but there is a need for expansion of the tools involved, including the development of universal cloning toolkits for the efficient and rapid assembly of mutants and transgenic expression strains, and the need for adaptation of genetic engineering tools to a wider range of microalgae species.

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“…Recently, the induction of PC biosynthesis was shown in Sinapis alba under treatment with platinum (Pt), rhodium (Rh), and palladium (Pd) [65], and in Z. mays seedlings in the presence of vanadium (V) [122]. Although PC biosynthesis can be induced in vivo by various elements, they are mainly involved in the detoxification of Cd and As (III) [14,54,99,[123][124][125][126][127] and, to a lesser extent, Hg [57,60,109,128,129], Pb [62,74,[130][131][132][133], Zn [54,55,74,133,134], Cu [55,74,119,135,136], and Sb(V) [69], which is partly determined by the stability of the metal complexes with S-containing ligands. Boron (B) [137], magnesium (Mg), calcium (Ca), and sodium (Na) [138] did not induce the biosynthesis of PCs.…”

Section: Pc Family Peptide Structure Identificationmentioning

confidence: 99%

“…Phytochelatin synthases are evolutionarily conserved in different species of higher plants and charophytes [218] and consist of 452-545 amino acid residues with a characteristic but variable C-terminal domain called the Phytochelatin_C domain [112,219,220]. It contains numerous Cys residues involved in metal binding and determining the increased stability of the protein as well as broad metal specificity [36,52,57,138,[219][220][221][222][223]. Different regions of the C-terminal domain of AtPCS1 in A. thaliana are important for its activation by Cd, Hg, Zn, and As (III) ions [54,219,221,224].…”

Section: Phytochelatin Synthase Is a Key Enzyme In The Biosynthesis O...mentioning

confidence: 99%

Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants

Серегин

Кожевникова

2023

IJMS

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Phytochelatins (PCs) are small cysteine-rich peptides capable of binding metal(loid)s via SH-groups. Although the biosynthesis of PCs can be induced in vivo by various metal(loid)s, PCs are mainly involved in the detoxification of cadmium and arsenic (III), as well as mercury, zinc, lead, and copper ions, which have high affinities for S-containing ligands. The present review provides a comprehensive account of the recent data on PC biosynthesis, structure, and role in metal(loid) transport and sequestration in the vacuoles of plant cells. A comparative analysis of PC accumulation in hyperaccumulator plants, which accumulate metal(loid)s in their shoots, and in the excluders, which accumulate metal(loid)s in their roots, investigates the question of whether the endogenous PC concentration determines a plant’s tolerance to metal(loid)s. Summarizing the available data, it can be concluded that PCs are not involved in metal(loid) hyperaccumulation machinery, though they play a key role in metal(loid) homeostasis. Unraveling the physiological role of metal(loid)-binding ligands is a fundamental problem of modern molecular biology, plant physiology, ionomics, and toxicology, and is important for the development of technologies used in phytoremediation, biofortification, and phytomining.

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Phytochelatin-mediated metal detoxification pathway is crucial for an organomercurial phenylmercury tolerance in Arabidopsis

Cited by 11 publications

References 53 publications

Synergistic Reduction of Arsenic Uptake and Alleviation of Leaf Arsenic Toxicity in Maize (Zea mays L.) by Arbuscular Mycorrhizal Fungi (AMF) and Exogenous Iron through Antioxidant Activity

Synergistic Reduction of Arsenic Uptake and Alleviation of Leaf Arsenic Toxicity in Maize (Zea mays L.) by Arbuscular Mycorrhizal Fungi (AMF) and Exogenous Iron through Antioxidant Activity

A synthetic biology approach for the treatment of pollutants with microalgae

Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants

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