Phyto-stimulating activity of the paracetamol-containing waste biodestruction product

Abstract

Currently, the search for effective ways to dispose of pharmaceutical waste, including the use of microorganisms, is relevant, which makes it possible to obtain products with new useful, in particular, phytostimulating properties. Our previous studies have shown that the product of bacterial destruction of paracetamol exhibits pronounced phytostimulating properties in relation to medicinal plants of the Lamiaceae, Plantaginaceae, Urticaceae, Asteraceae families and can be used as an inducer of accumulation of biologically active substances in them.
 The purpose of this work is to investigate the effect of the biodegradation product of paracetamol on the amount of polysaccharides, biomass, size and morphological and anatomical features of flax seeds Linum usitatissimum L. (Linaceae family).
 Materials and methods. The work used a product of biodegradation of paracetamol (PBP), obtained on the basis of the laboratory of alkanotrophic microorganisms "Institute of Ecology and Genetics of Microorganisms UrO RAS (Perm) from the pharmaceutical substance paracetamol with an expired expiration date. The study of the phytostimulating effect of PBP in relation to common flax was carried out on the basis of the A.G. Genkel Botanical Garden of the Perm State National Research University. The content of polysaccharides was determined by gravimetric method. A comparative analysis of the morphological and anatomical structure of seeds was carried out using the Motic DM-111 digital microscope and the Motic Play and Motic Educator software.
 Results. When processing flax seedlings of ordinary PBP, the amount of polysaccharides in seeds increased by 6%, the total collection of seed biomass by 20.5%, the size (length) of seeds by 2% compared to the control (water). Biometric parameters of the mucous layer of seeds collected from the site treated with PBP increased by 10% compared to the control.
 Conclusion. The biodegradation product of paracetamol has a stimulating effect on the medicinal plant of the flax family (Linaceae) flax Linum usitatissimum L., increasing the content of polysaccharides, the total collection of seed biomass and their size.