Phyto/rhizoremediation studies using long-term PCB-contaminated soil

Macková, Martina; Prouzová, Petra; Stursa, Petr; Ryslavá, Edita; Uhlík, Ondřej; Beranova, Katarina; Rezek, Jan; Kurzawova, Veronika; Demnerova, Kateřina; Macek, Tomáš

doi:10.1007/s11356-009-0240-3

Cited by 72 publications

(28 citation statements)

References 54 publications

(52 reference statements)

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“…Samples of horseradish rhizosphere soil were procured after 5 months and 11 months of replicate pot cultivation of horseradish plants in long-term PCB-contaminated soil as described elsewhere (72). The soil used was sandy soil contaminated mainly by Delor 103 and Delor 106 mixtures of PCBs, consisting of congeners with levels of chlorination similar to those for Aroclor 1242 and 1260, respectively (43).…”

Section: Methodsmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Uhlík

Strejcek

Junková

et al. 2011

Appl Environ Microbiol

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Bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (PCB)-contaminated soil vegetated by horseradish. Isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The usage of MALDI Biotyper for the classification of isolates was evaluated and compared to 16S rRNA gene sequence analysis. A wide spectrum of bacteria was isolated, with Arthrobacter, Serratia, Rhodococcus, and Rhizobium being predominant. Arthrobacter isolates also represented the most diverse group. The use of MALDI Biotyper in many cases permitted the identification at the level of species, which was not achieved by 16S rRNA gene sequence analyses. However, some isolates had to be identified by 16S rRNA gene analyses if MALDI Biotyper-based identification was at the level of probable or not reliable identification, usually due to a lack of reference spectra included in the database. Overall, this study shows the possibility of using MALDI-TOF MS and MALDI Biotyper for the fast and relatively nonlaborious identification/classification of soil isolates. At the same time, it demonstrates the dominant role of employing 16S rRNA gene analyses for the identification of recently isolated strains that can later fill the gaps in the protein-based identification databases.Bacterial identification has always been a major challenge in all microbiological fields. Originally, all bacteria were identified according to their phenotypic characteristics after they had been isolated in pure culture. Only in the 1980s did scientists discover a huge discrepancy in the bacterial contents in aquatic and terrestrial habitats expressed as CFU after cultivation and direct microscopic enumeration (62). This discovery contributed highly to the boom in molecular biological methods of bacterial identification, which are independent of cultivation. These techniques have enabled scientists to discover and study actual bacterial diversity, which in most environments is orders of magnitude larger than culture-based techniques are able to reveal (40). However, characterization of bacteria after isolation still remains the most effective way of identifying bacterial properties.The necessity of fast and accurate identification of bacteria has been driven mostly by the needs of clinical and food microbiology, as these fields are directly connected with human health. Whereas tests based on biochemical traits are still important for the identification of pathogenic bacteria (64), they mainly fail with microbes isolated from environmental samples, as the diversity of microbes in these habitats is enormous (70). Environmental isolates are usually classified based on the primary structures of 16S rRNA genes (9). The process of DNA isolation, amplification of target genes, and sequencing is, however, rather time-consuming. Therefore, the introduction of mass spectrometry (MS) for the purposes of bacterial identification (13,24) represented an important ...

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Section: Methodsmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Uhlík

Strejcek

Junková

et al. 2011

Appl Environ Microbiol

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“…Consequently, each plant species stimulates bacterial activity and ability to degrade PCBs with different efficiencies. Previously Mackova et al [14] showed the results of phytoremediation studies with different plants (tobacco, nightshade, alfalfa, horseradish) in experiments carried out within several years. They summarized that tobacco and horseradish themselves mostly proved beneficial phytoremediation effect; nevertheless, this information cannot be generalized for neither all plant species nor for the same species cultivated under different conditions.…”

Section: Discussionmentioning

confidence: 99%

“…It was procured from a dumpsite of contaminated soil in Lhenice, south Bohemia [14]. Nine different microcosms were constructed using 300 g of contaminated soil, two different plant species (tobacco and nightshade) and various bacterial strains (strains of Pseudomonas spp.…”

Section: Sample Preparationmentioning

confidence: 99%

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Plant–microorganism interactions in bioremediation of polychlorinated biphenyl-contaminated soil

et al. 2012

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“…Rhizoremediation uses the symbiosis between plants and microbes in the rhizosphere to degrade organic compounds to non-toxic or less toxic compounds (Mackova et al, 2009;Slater et al, 2011). This task is based on the ability of the roots of certain plants to induce the growth and metabolism of pollutant-degrading microorganisms (Aken et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Bioaugmentation with Immobilized Microorganisms to Enhance Phytoremediation of PCB-Contaminated Soil

Pino

Muñera

Peñuela

2016

Soil and Sediment Contamination: An International Journal

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The aim of this study was evaluate the effect of bioaugmentation by free and immobilized strains of microbial consortium on the phytoremediation of polychlorinated biphenyl (PCB)-contaminated soil using the Avena sativa, Brachiaria decumbens, Brassica juncea, and Medicago sativa plants. Alginate and biochar were used as carrier materials and free cells were used as the control. PCBs 44, 66, 118, 138, 153, 170, and 180 were chosen as indicator PCB congeners. After 60 days of plant growth, the concentration of each congener and the survival of the microbial inoculum were evaluated. The removal of the PCB congener was greater in Brassica juncea planted treatments and using biochar as a carrier material. PCB 66 was the congener with the highest removal percentage in all using biochar and alginate-immobilized microorganisms and free microorganisms, while PCB 170 had the lowest removal percentage in all treatments. The largest removal percentage for all congeners was obtained using biochar as a carrier material (7.2% to 30.3%) and the lowest with planted treatments using free Downloaded by [University of California, San Diego] at 07:15 28 March 2016 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT3 microorganisms (2.3% to 6.8%). Real-time polymerase chain reaction (PCR) showed that the microbial inoculum survived when it was immobilized using both alginate and biochar without any significant differences between treatments; however, PCB removal percentages were obtained with biochar, which demonstrated that this carrier material has a positive effect on microbial activity.

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Phyto/rhizoremediation studies using long-term PCB-contaminated soil

Cited by 72 publications

References 54 publications

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

Plant–microorganism interactions in bioremediation of polychlorinated biphenyl-contaminated soil

Bioaugmentation with Immobilized Microorganisms to Enhance Phytoremediation of PCB-Contaminated Soil

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