Phytic acid transport in Phaseolus vulgaris: A new low phytic acid mutant in the PvMRP1 gene and study of the PvMRPs promoters in two different plant systems

Cominelli, Eleonora; Confalonieri, Massimo; Carlessi, Martina; Cortinovis, Gaia; Daminati, Maria Gloria; Porch, Timothy G.; Losa, Alessia; Sparvoli, Francesca

doi:10.1016/j.plantsci.2018.02.003

Cited by 40 publications

(42 citation statements)

References 78 publications

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“…On the other hand, phytic acid reduction on germinated vetches could be linked to an increase in phytase activities. In fact, this enzyme makes the phytates soluble to support seedling growth [50]. Similar results were found on chickpea after 48 h of germination, where phytic acid content was reduced on 59% [51].…”

Section: Effect Of Germination On Nnf Of Vetchessupporting

confidence: 72%

Effect of Instant Controlled Pressure-Drop (DIC), Cooking and Germination on Non-Nutritional Factors of Common Vetch (Vicia sativa spp.)

et al. 2019

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Legumes are widely consumed by humans, being an important source of nutrients; however, they contain non-nutritional factors (NNFs), such as phytic acid (IP6), raffinose, stachyose, total phenolic compounds, condensed tannins, and flavonoids, that have negative effects on human health. Although vetches (Vicia sativa) are widely cultivated, they are not intended for human feeding due to their contents of NNF. Usually, the NNF are removed by cooking or germinating; however, germination is a process that requires extended time, and cooking may compromise the viability of some nutrients. To promote vetches for human consumption, the effect of the Instant Controlled Pressure Drop (DIC) process was studied as an alternative to cooking and germinating to decrease NNF contents. Results showed that compared to raw vetches, DIC treatment reduced total phenolic compounds (48%), condensed tannins (28%), flavonoids (65%), IP6 (92%), raffinose (77%), and stachyose (92%). These results are very similar to the ones achieved by traditional ways of removing NNF.

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Section: Effect Of Germination On Nnf Of Vetchessupporting

confidence: 72%

Effect of Instant Controlled Pressure-Drop (DIC), Cooking and Germination on Non-Nutritional Factors of Common Vetch (Vicia sativa spp.)

et al. 2019

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“…In the recent past, low-phytate lines in pulse crops have been developed and characterized to reduce the concentration of phytate and thus improve mineral absorption [225,[230][231][232][233]. Warkentin et al [225] developed low-phytate pea lines, 1-150-81 and 1-2347-144, using chemical mutagenesis of cultivar CDC Bronco, a high-performing pea variety.…”

Section: Challenges and Future Strategies For Biofortificationmentioning

confidence: 99%

“…They further observed that a defective Mrp1 gene caused an lpa1 mutation in common beans that downregulates the phytic acid pathway at the transcriptional level and thus reduced seed myo-inositol. Recently, a new lpa line influencing the PvMRP1 phytic acid transporter was identified in common beans using ethyl methane sulfonate mutagenesis [233]. Further, PvMRP promoters were characterized in Arabidopsis thaliana and Medicago truncatula transgenic plants.…”

Section: Challenges and Future Strategies For Biofortificationmentioning

confidence: 99%

Biofortification of Pulse Crops: Status and Future Perspectives

Jha

Warkentin

2020

Plants

140

108

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Biofortification through plant breeding is a sustainable approach to improve the nutritional profile of food crops. The majority of the world’s population depends on staple food crops; however, most are low in key micronutrients. Biofortification to improve the nutritional profile of pulse crops has increased importance in many breeding programs in the past decade. The key micronutrients targeted have been iron, zinc, selenium, iodine, carotenoids, and folates. In recent years, several biofortified pulse crops including common beans and lentils have been released by HarvestPlus with global partners in developing countries, which has helped in overcoming micronutrient deficiency in the target population. This review will focus on recent research advances and future strategies for the biofortification of pulse crops.

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“…The main difference between cereals and legumes for which PA-MRPs have been characterized so far is the gene number: While only one gene is present in diploid maize, pearl millet, and rice genomes, and three copies in the hexaploid Triticum aestivum, two and three paralogues are present in common bean and soybean, respectively [17,32,33]. The presence of more than one member of the PA-MRP genes seems to be a common feature of legumes, for example also in Medicago truncatula it is possible to predict two PA-MRP genes [35], unlike the situation in other dicotyledons, such as Arabidopsis and Solanum lycopersicum L. (tomato) in which only one PA-MRP gene was described [16,36]. As discussed below, the gene copy number has a significant influence on the lpa mutant phenotypes.…”

Section: Pa-mrp Transportersmentioning

confidence: 99%

“…The PvMRP2 gene, ortholog of GmMRP13, is expressed similarly to PvMRP1 in vegetative organs, but at no appreciable level in cotyledons. Interestingly, both genes are expressed in root nodules, organs specialized in symbiosis with nitrogen-fixing bacteria, in which the role of PA is still unknown [35]. Recently, a detailed analysis was reported of GUS activity in Arabidopsis thaliana and Medicago truncatula plants, harboring a promoter sequence of PvMRP1 and PvMRP2 genes, fused upstream of the GUS reporter gene.…”

Section: Gmmrp13/gmabcc3mentioning

confidence: 99%

Phytic Acid and Transporters: What Can We Learn from low phytic acid Mutants?

Cominelli

Pilu

Sparvoli

2020

Plants

Self Cite

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Phytic acid has two main roles in plant tissues: Storage of phosphorus and regulation of different cellular processes. From a nutritional point of view, it is considered an antinutritional compound because, being a cation chelator, its presence reduces mineral bioavailability from the diet. In recent decades, the development of low phytic acid (lpa) mutants has been an important goal for nutritional seed quality improvement, mainly in cereals and legumes. Different lpa mutations affect phytic acid biosynthetic genes. However, other lpa mutations isolated so far, affect genes coding for three classes of transporters: A specific group of ABCC type vacuolar transporters, putative sulfate transporters, and phosphate transporters. In the present review, we summarize advances in the characterization of these transporters in cereals and legumes. Particularly, we describe genes, proteins, and mutants for these different transporters, and we report data of in silico analysis aimed at identifying the putative orthologs in some other cereal and legume species. Finally, we comment on the advantage of using such types of mutants for crop biofortification and on their possible utility to unravel links between phosphorus and sulfur metabolism (phosphate and sulfate homeostasis crosstalk).

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Phytic acid transport in Phaseolus vulgaris: A new low phytic acid mutant in the PvMRP1 gene and study of the PvMRPs promoters in two different plant systems

Cited by 40 publications

References 78 publications

Effect of Instant Controlled Pressure-Drop (DIC), Cooking and Germination on Non-Nutritional Factors of Common Vetch (Vicia sativa spp.)

Effect of Instant Controlled Pressure-Drop (DIC), Cooking and Germination on Non-Nutritional Factors of Common Vetch (Vicia sativa spp.)

Biofortification of Pulse Crops: Status and Future Perspectives

Phytic Acid and Transporters: What Can We Learn from low phytic acid Mutants?

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