Phytases and Phosphatases of Thermophilic Microbes: Production, Characteristics and Multifarious Biotechnological Applications

Singh, Bijender; Satyanarayana, T.

doi:10.1007/978-94-007-5899-5_25

Cited by 1 publication

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“…However, monogastric species including poultry, pigs, fish and humans cannot produce phytases, or produce it in low quantities. Therefore, phytic acid is unavailable to these species; phytases or Pi is added as a supplement to the feed of these animals (Singh et al, 2011;Singh and Satyanarayana, 2015;Jing et al, 2021). However, adding Pi is not sustainable, because Pi is not a renewable material (Secco et al, 2017).…”

Section: -mentioning

confidence: 99%

Metallophosphoesterases of Chlamydomonas reinhardtii and Analyses of Their Transcription Levels Under Phosphate Deficiency

Havutcu

Aksoy

2023

JAST

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Phosphorous (P) is an important macroelement for all organisms. However, there is a finite amount of P on Earth, therefore, new enzymes and technologies are needed for better P use in agriculture. Metallophosphoesterases are a large group of evolutionarily related proteins that are important in biotechnology. We found fourteen putative Metallophosphoesterase (MPA) genes in the genome of Chlamydomonas reinhardtii. Our RT-PCR analyses showed that some of these genes were constitutively expressed, and some were upregulated under phosphate deficiency. These results and bioinformatic analyses suggest that two of the genes (MPA11 and MPA13) are transcribed in high levels and the putative polypeptides are predicted to be secreted to extracellular space, making them ideal to be used in biotechnological applications. Phylogenetic analyses show that MPA11 and MPA13 are related to known phytases from plant species, suggesting MPA11 and MPA13 might have specific phytase activity. In light of these results, we discuss the potential of C. reinhardtii as a phytase producing organism for agricultural and industrial use.

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Section: -mentioning

confidence: 99%