Phytase Production from a Novel Klebsiella sp. on Wheat Bran for Animal Feed Digestion

Abstract

Background. The present study was aimed to isolate phytase producing bacteria and optimize the physicochemical parameters of their phytase production. Materials and methods. Four bacterial isolates (Phs4, Phs5, Phs6, and Phs8), based on clear zone formation on phytase screening medium, were selected and tested for finding out the highest phytase producing strain. The production of phytase was then optimized and its biochemical properties were determined to judge the applicability of phytase as a digestive aid in animal feed. Results. The 4 bacterial isolates (Phs4, Phs5, Phs6 and Phs8) were identified by morphological, cultural, biochemical and molecular characterization as Burkholderia cepacia, Escherichia coli, Klebsiella pneumoniae and Klebsiella sp. respectively. Of these isolates, Phs8 (Klebsiella sp.) was found to produce maximum phytase in shake culture in a basal medium containing Na-phytate at 37 o C and pH 5.5 after 72 hours of incubation. The omission of Na-phytate from the medium almost completely abolished the phytase production capacity of the isolate and thus signified its important role as an inducer. Among the different complex carbon sources, viz., glucose, wheat bran, rice bran and chickpea, maximum phytase production (94 unit/ ml) was obtained with wheat bran under comparable cultivation conditions. The phytase works best at a temperature of 37 o C and pH of 4.0 with a wide temperature stability (more than 80% activity up to 80 o C) and wide pH stability (more than 80% activity within a range of pH 3-8). Although Zn 2+ , Co 2+ , and Fe 2+ slightly increased the phytase activity Cu 2+ and Mg 2+ strongly inhibited the enzyme. Conclusion. The present findings will be very useful for the development of a bioprocess of the enzyme for its large-scale production at the pilot and finally at the commercial level.

Review on Desirable Microbial Phytases as a Poultry Feed Additive: Their Sources, Production, Enzymatic Evaluation, Market Size, and Regulation

“…It has nutrients that microbes need, including minerals (Ca, K, Mg, and Fe), B vitamins (thiamin, niacin, riboflavin, and folate), insoluble fiber, essential fatty acid, starch, protein, and phytic acid [ 67 ]. Wheat bran can, therefore, induce and promote the production of phytase [ 52 , 58 ]. Maximum phytase production (94 U/mL) was obtained with wheat bran SmF by Klebsiella sp compared to the SmF of rice bran and chickpea by the same bacterial strain [ 58 ].…”

“…Wheat bran can, therefore, induce and promote the production of phytase [ 52 , 58 ]. Maximum phytase production (94 U/mL) was obtained with wheat bran SmF by Klebsiella sp compared to the SmF of rice bran and chickpea by the same bacterial strain [ 58 ]. From wheat bran SSF by Schizophyllum commune [ 26 ] and by A. niger NT7 [ 11 ], the highest phytase production of 113.7 U/gds and 208.30 ± 0.22 U/gds, respectively, was reported.…”

Unraveling the potential of bacterial phytases for sustainable management of phosphorous

“…However, in past few decades, SSF technology, because of being simple, more economical, and environment friendly over SmF, has also attracted many researchers for large-scale production of bacterial phytases. 134 Akter et al 135 studied the production of phytase from Klebsiella sp. using different substrates including glucose, wheat bran, rice bran, and chickpea.…”