Physiopathological Roles of Human Transglutaminase 2

Expression of Functional Recombinant Human Tissue Transglutaminase (TG2) Using the Bac-to-Bac Baculovirus Expression System

“…TG2 might be an attractive novel pharmacological target for treatment of neurodegenerative diseases in preventing toxic protein aggregation. 13 Although many studies about the diverse aspects of tissue transglutaminase (TG2) specially its roles in pathobiology of diseases have been reported, 2,7,14 there have not been much reports on the enzyme inhibitors. For this reason the expression of recombinant human TG2 enzyme as well as the assessment of its in vitro biological activity has been undertaken in the present study.…”

“…The enzyme has been found in the cytosol and other cellular compartments such as mitochondria, 5 and extracellular matrix. TG2, as a multifunctional protein, has a number of calcium dependent enzymatic activities such as transamidation/crosslinking 6,7 (Table 1). TG2 interacts non-covalently with several cellular and extracellular matrix (ECM) proteins such as fibronectin, 4 integrin, 6 and heparan sulfates.…”

“…8 The enzyme has been involved (calcium independently) in multiple cellular processes for instance cellular proliferation and signal transduction. 5,9 TG2 has also been implicated in some of diseases such as celiac disease, 10 certain types of cancer, 7 and several neurodegenerative diseases including Alzheimer's disease (AD) ( Table 1). 11,12 TG2 has been found as a novel pharmacological target in neurodegenerative diseases.…”