Physiology of the osmotic stress response in microorganisms

Gutierrez, Claude; Abee, Tjakko; Booth, Ian R.

doi:10.1016/0168-1605(95)00059-3

Cited by 74 publications

(50 citation statements)

References 56 publications

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“…In all, our results demonstrate that in E. faecalis, exogenously supplied compounds ensuring osmoprotection are restricted to the betaines and their analogues. In contrast, most of the bacteria studied so far accept both betaines and imino acids such as proline, pipecolate or ectoine as effective osmoprotectants (Csonka & Hanson, 1991 ; Gouesbet et al, Gutierrez et al, 1995;Jebbar et al, 1997).…”

Section: Gb and Its Structural Analogues But Not Other Osmoprotectanmentioning

confidence: 99%

“…One of the most powerful adaptive strategies bacterial cells have evolved to counteract low water activities of IP: 54.149.104.195 On: Wed, 09 May 2018 22:55:57 V. PICHEREAU a n d OTHERS their growth media is the accumulation to high intracellular levels of a set of organic solutes that are synthesized de nouo or actively taken up from the growth medium (Csonka & Hanson, 1991 ;Gutierrez et al, 1995;Csonka & Epstein, 1996). The exogenous osmoprotectants belong to a few classes of organic compounds that are neutral at physiological pH and compatible with cellular functions (Brown, 1976).…”

Section: Introductionmentioning

confidence: 99%

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The osmoprotectant glycine betaine inhibits salt-induced cross-tolerance towards lethal treatment in Enterococcus faecalis

et al. 1999

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The response of €nterococcus faecalis ATCC 19433 to salt stress has been characterized previously in complex media. In this report, it has been demonstrated that this bacterium actively accumulates the osmoprotectant glycine betaine (GB) from salt-enriched complex medium BHI. To further understand the specific effects of GB and other osmoprotective compounds in salt adaptation and salt-induced cross-tolerance to lethal challenges, a chemically defined medium lacking putative osmoprotectants was used. In this medium, bacterial growth was significantly reduced by increasing concentrations of NaCI. A t 075 M NaCI, 90% inhibition of the growth rate was observed; GB and its structural analogues restored growth to the non-saltstressed level. In contrast, proline, pipecolate and ectoine did not allow growth recovery of stressed cells. Kinetic studies showed that the uptake of betaines shows strong structural specificity and occurs through a salt-stressinducible high-affinity porter [K, = 3 3 pM; V-= 130 nmol min" (mg protein)''; the uptake activity increased 400-fold in the presence of 0.5 M NaCI]. Moreover, GB and its analogues were accumulated as non-metabolizable cytosolic osmolytes and reached intracellular levels ranging from 1.3 t o 1.5 pmol (mg protein)". In contrast to the beneficial effect of GB on the growth of salt-stressed cultures of €. faecalis, its accumulation inhibits the salt-induced cross-tolerance to a heterologous lethal challenge. Indeed, pretreatment of bacterial cells with 0 5 M NaCl induced resistance to 0.3% bile salts (survival of adapted cells increased by a factor of 6800). The presence of GB in the adaptation medium reduced the acquisition of bile salts resistance 680-fold. The synthesis of I 1 of the 13 proteins induced during salt adaptation was significantly reduced in the presence of GB. These results raise questions about the actual beneficial effect of GB in natural environments where bacteria are often subjected to various stresses.

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Section: Gb and Its Structural Analogues But Not Other Osmoprotectanmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The osmoprotectant glycine betaine inhibits salt-induced cross-tolerance towards lethal treatment in Enterococcus faecalis

et al. 1999

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“…trolytes e.g. K glutamate but accumulation of organic solutes that are more compatible with cell Ž physiology appears to be preferred Galinski, 1995;Gutierrez et al, 1995;Hagemann et al, 1999;Kempf and Bremer, 1998;Miller and Wood, 1996;Poolman and Glaasker, 1998;Smith et al, . 1998;Ventosa et al, 1998;Wood, 1999 . Compatible solutes fall into a few structural Ž .…”

Section: Introductionmentioning

confidence: 99%

Osmosensing and osmoregulatory compatible solute accumulation by bacteria

Wood

Bremer

Csonka

et al. 2001

Comparative Biochemistry and Physiology Part A: Molecular &

408

352

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Osmosensing and osmoregulatory compatible solute accumulation by bacteria Wood, J.M.; Bremer, Erhard; Csonka, L.N.; Kraemer, R; Poolman, B.; van der Heide, Tiemen; Smith, L.T. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. AbstractBacteria inhabit natural and artificial environments with diverse and fluctuating osmolalities, salinities and temperatures. Many maintain cytoplasmic hydration, growth and survival most effectively by accumulating kosmotropic organic Ž . Ž . solutes compatible solutes when medium osmolality is high or temperature is low above freezing . They release these solutes into their environment when the medium osmolality drops. Solutes accumulate either by synthesis or by transport from the extracellular medium. Responses to growth in high osmolality medium, including biosynthetic accumulation of trehalose, also protect Salmonella typhimurium from heat shock. Osmotically regulated transporters and mechanosensitive channels modulate cytoplasmic solute levels in Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Lactobacillus plantarum, Lactococcus lactis, Listeria monocytogenes and Salmonella typhimurium. Each organism harbours multiple osmoregulatory transporters with overlapping substrate specificities. Membrane proteins Ž that can act as both osmosensors and osmoregulatory transporters have been identified secondary transporters ProP of . E. coli and BetP of C. glutamicum as well as ABC transporter OpuA of L. lactis . The molecular bases for the modulation of gene expression and transport activity by temperature and medium osmolality are under intensive investigation with emphasis on the role of the membrane as an antenna for osmo-andror thermosensors. ᮊ 2001 Elsevier Science Inc. All rights reserved. Wood et al. r Comparati¨e Biochemistry and Physiology Part A 130 2001 437᎐460 438

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“…A. hydrophila incubated in marine water for 30 days became susceptible to two antibiotics, rifampicin and streptomycin (table 3). its increasing susceptibility may be due to alteration of the bacterial membrane as induced by the hyper osmotic conditions (GUtiErrEz et al, 1995). for the same strain incubated in marine water (during 30 days), after a pretreatment of 24 h in waste water, we noted that A. hydrophila lost susceptibility to three other antibiotics: chloramphenicol, oxalinic acid and trimethoprim-sulfamethoxazole.…”

Section: Resultsmentioning

confidence: 93%

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

Mejri

Bour

Boukef

et al. 2008

rseau

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AbstractThe occurrence of the mesophilic motile Gram negative non enterobacterial speciesA. hydrophilain the wild is a major problem that deserves to be resolved since it is a potentially pathogen able to enter into a non-culturable state on routine bacteriological plating media. These non-culturable forms can be detected by several direct or indirect visualization methods. This species has frequently been isolated from pathological forms in fish farming marine areas, especially near wastewater discharges. Consequently, we studiedA. hydrophilain marine water microcosms placed during a 24 hour period in treated waste waters and compared with the homologous strain not placed in the same conditions. Thus, two kinds of microcosms were prepared using filtered and autoclaved marine water or waste water, inoculated byA. hydrophilaand maintained at 25°C in darkness. The results obtained indicated thatA. hydrophilapopulation incubated at 25°C in marine water declined rapidly (3.21 log units in plate count number) during the first three days. Additionally, we noted thatA. hydrophilaincubated in marine water after a previous treatment in waste water declined progressively to 2.74 log units (in plate count number). Nevertheless, we showed no significant variations of the number of total bacterial cells forA. hydrophiladeveloped in marine water after prior treatment in waste water, despite the appearance of the VBNC form. During this state, rods of normal size, elongated cells and cocci were obtained. Concomitantly, we determined several changes in biochemical and antimicrobial patterns of stressedA. hydrophila, notably the acquisition of adipate metabolization and an increase of resistance to antimicrobial compounds, especially forA. hydrophilaincubated in marine water after treatment in waste water.

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Physiology of the osmotic stress response in microorganisms

Cited by 74 publications

References 56 publications

The osmoprotectant glycine betaine inhibits salt-induced cross-tolerance towards lethal treatment in Enterococcus faecalis

The osmoprotectant glycine betaine inhibits salt-induced cross-tolerance towards lethal treatment in Enterococcus faecalis

Osmosensing and osmoregulatory compatible solute accumulation by bacteria

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

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