Physiology of the Ciliate Colpidium colpoda. I. The Effect of Various Bacteria as Food on the Division Rate of Colpidium colpoda

Burbanck, W. D.

doi:10.1086/physzool.15.3.30151646

Cited by 36 publications

(15 citation statements)

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“…In the living protozoon the spine extends straight backwards from the posterior end of the organism. The spine was still present with the same configuration after 3 months in culture, although Clarke (1963) found that mixed epidinia grown in vitro lost these spines after 7 months in culture. This protozoon is referred to hereafter as the 'cattle form' of E. ecaudatum caudatum.…”

Section: Morphologymentioning

confidence: 90%

The Cultivation of the Rumen Ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro

Coleman

Davies

Cash

1972

Journal of General Microbiology

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SUMMARYPopulations of over 400 Epidiniirm ecaudatum caudatumlml were maintained for over I 8 months in the presence of bacteria, in a potassium phosphate-rich medium under 95 % N,+ 5 % CO, with the daily addition of wholemeal flour and dried grass. Polyplastron multivesiculatum was also maintained at a population density of 45 to 70/ml under the same conditions, except that it was necessary to add each day a culture of another protozoon such as Epidinium ecaudatum caudatum, E. ecaudatum tricaudatum or Eudiplodinium maggii to give a population density of approximately roo/ml. The epidinia at least were engulfed by the polyplastron up to 10 being taken up each day; the minimum daily requirement for growth was one epidinium/polyplastron. Polyplastron multivesiculatum also grew in the absence of other protozoa on a sodium chloride-rich medium, and if these protozoa were inoculated into the potassium phosphate-rich medium in the presence of epidinia, there was a lag of 3 to 5 days before they began to engulf the epidinia. The effect of other variations in the culture conditions is also described. The present paper describes the cultivation of Epidinium ecaudatum caudatum and Polyplastron inultivesiculatum in the presence of bacteria in buffered salts medium with wholemeal flour and dried grass as the sources of carbohydrate. Under certain conditions P. multivesiculatum had an absolute requirement for the epidinia for growth and died rapidly without them.Preliminary communications gave the growth requirements of these ciliates immediately after their isolation from the rumen (Coleman, 1970(Coleman, , 1971).

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Section: Morphologymentioning

confidence: 90%

The Cultivation of the Rumen Ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro

Coleman

Davies

Cash

1972

Journal of General Microbiology

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“…Many early studies noted that for a given ciliate some species of bacteria were suitable food organisms while other were not [95]. Some bacteria in fact were toxic to ciliates [100,101].…”

Section: Culturing Freshwater Ciliates With Bacteriamentioning

confidence: 99%

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Pinheiro¹,

Bols²

2013

Springer Science Reviews

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How the ciliates of fish can be cultured and be used to study ciliate/fish interactions are reviewed. The culturing of ciliates is currently done in basal solutions based on either freshwater, seawater, or bodily fluids of vertebrates. These are supplemented either with bacteria, fish cells, or organic matter, which can be defined or more commonly undefined, with proteose peptone being a prominent example. Among pathogenic ciliates, the most difficult to culture has been Ichthyophthirius multifiliis. To contrast, Cryptocaryon irritans caused a similar disease and has been maintained successfully in co-cultures with fish cells. Pathogenic scuticociliates and tetrahymenas are more amenable to culture, and can be grown axenically. These cultures have been used to study pathogenic mechanisms and to screen drugs for their potential chemotherapeutic value. Ciliates may act directly on fish to cause disease, but may also influence fish health indirectly through their interactions with other types of fish pathogens, for example bacteria and fungi. For example, in culture Tetrahymena spp. have been shown to phagocytose pathogenic bacteria, Yersinia ruckerii, and microsporidia, Glugea hertwigi, where ciliates are not linked to disease, very different ciliate/fish interactions are possible. For some fish larvae, free-living ciliates are a source of nutrients. Large-scale cultures of both freshwater and marine ciliates have been achieved and could be a source of feed for fish larvae in aquaculture. Finally, ciliates have the potential to feed on fish carcasses and in doing so make nutrients available to the ecosystem. In the future cell cultures should be invaluable in studying these and other possible relationships between fish and ciliates.

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“…Remarkable physiological studies done by Burbanck (1942) and Burbanck & Gilpin (1946). Growth has been studied by MucibabiC (1953).…”

Section: Genus Colpidium Stein 1860mentioning

confidence: 99%

Taxonomy and ecology of some ciliates (Protozoa, Ciliophora) of the saprobic system. III. Revision of the genera Colpidium and Dexiostoma, and establishment of a new genus, Paracolpidium nov. gen.

Ganner

Foissner

1989

Hydrobiologia

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The genera Colpidium Stein, 1860 and Dexiostoma Jankowski, 1967 are revised. Our monographic treatment is based on a morphologic and biometric reinvestigation of the main species of these genera and on an evaluation of data from the literature. Colpidium comprises four species, namely C. colpoda (Losana, 1829), C. singulare Vuxanovici, 1961, C. acuminatum Vuxanovici, 1962, and C. kleini Foissner, 1969. The monotypic genus Dexiostoma contains D. campyla (Stokes, 1886) and is distinguished from Colpidium by a different oral infraciliature and the position and shape of the preoral suture. A new genus, Paracolpidium nov. gen., is suggested for Colpidium truncatum Stokes, 1885 because its oral infraciliature deviates from both Colpidium and Dexiostoma. Contrary to Colpidium and Dexiostoma, the silverline system of Paracolpidium truncatum lacks secondary meridians but shows short projections to the left of the primary meridians indicating a phylogenetic relationship to Tetrahymena.

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Physiology of the Ciliate Colpidium colpoda. I. The Effect of Various Bacteria as Food on the Division Rate of Colpidium colpoda

Cited by 36 publications

References 0 publications

The Cultivation of the Rumen Ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro

The Cultivation of the Rumen Ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro

Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Taxonomy and ecology of some ciliates (Protozoa, Ciliophora) of the saprobic system. III. Revision of the genera Colpidium and Dexiostoma, and establishment of a new genus, Paracolpidium nov. gen.

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