Physiology of Immobilised Microbial Cells

Willaert, Ronnie; Nedović, Viktor; Baron, Gino V.

doi:10.1007/978-94-017-1638-3_24

Cited by 6 publications

(4 citation statements)

References 123 publications

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“…Evidence that the continuous mode of reactor operation, immobilization, aging and mutation of cells provokes different physiological responses when compared to batch free cell systems have been frequently observed 43,116 . These differences have to be taken into account when considering continuous beer fermentation (Table I).…”

Section: Particularities Of Continuous Fermentation Systemsmentioning

confidence: 99%

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A Review of Flavour Formation in Continuous Beer Fermentations*

Brányik

Vicente

Dostálek

et al. 2008

Journal of the Institute of Brewing

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The attractive prospect of a continuous beer fermentation system consists mostly of the accelerated transformation of wort into beer. Although continuous beer fermentation has been studied as a promising technology for several decades, the number of industrial applications is still limited. The major obstacle hindering the extensive industrial exploitation of this technology is the difficulty in achieving the correct balance of sensory compounds in the short time typical for continuous systems. This paper offers an integral view on the particularities of continuous systems, which may impart beer a sensorial character distinct from conventionally fermented counterparts. The main groups of flavour active compounds are discussed from the perspective of possible control strategies by means of process parameters and strain selection.

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Section: Particularities Of Continuous Fermentation Systemsmentioning

confidence: 99%

“…Changes in metabolic functions (substrate uptake, product formation, enzyme expression and activity) of the immobilized cells have been reported because of complex micro-environmental conditions resulting from cell immobilization 34,84,100,116 .…”

Section: Physiological Changes Caused By Immobilizationmentioning

confidence: 99%

A Review of Flavour Formation in Continuous Beer Fermentations*

Brányik

Vicente

Dostálek

et al. 2008

Journal of the Institute of Brewing

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“…For practical applications, the use of highly toxic cations such as Pb 2+ , Cu 2+ , and Cd 2+ is limited. The use of Sr 2+ and Ba 2+ , which are mildly toxic, has been reported in cell immobilization applications although only at low concentrations [ 131 ]. Ca 2+ is certainly the divalent cation most used to form ionic alginate gels for its good binding affinity to alginate and lack of toxicity under normal conditions of use [ 15 ].…”

Section: Methods For the Gelation Of Polymeric Droplets To Producementioning

confidence: 99%

Technologies and Formulation Design of Polysaccharide-Based Hydrogels for Drug Delivery

et al. 2020

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Polysaccharide-based hydrogel particles (PbHPs) are very promising carriers aiming to control and target the release of drugs with different physico-chemical properties. Such delivery systems can offer benefits through the proper encapsulation of many drugs (non-steroidal and steroidal anti-inflammatory drugs, antibiotics, etc) ensuring their proper release and targeting. This review discusses the different phases involved in the production of PbHPs in pharmaceutical technology, such as droplet formation (SOL phase), sol-gel transition of the droplets (GEL phase) and drying, as well as the different methods available for droplet production with a special focus on prilling technique. In addition, an overview of the various droplet gelation methods with particular emphasis on ionic cross-linking of several polysaccharides enabling the formation of particles with inner highly porous network or nanofibrillar structure is given. Moreover, a detailed survey of the different inner texture, in xerogels, cryogels or aerogels, each with specific arrangement and properties, which can be obtained with different drying methods, is presented. Various case studies are reported to highlight the most appropriate application of such systems in pharmaceutical field. We also describe the challenges to be faced for the breakthrough towards clinic studies and, finally, the market, focusing on the useful approach of safety-by-design (SbD).

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“…A number of reports proved that the physiology of immobilized cells may differ as compared to free cells, which may lead to higher tolerance of cells towards toxic compounds, increased plasmid stability of recombinant cells, improved enzyme expression as well as formation of micro-colonies with changed cell morphology and increased degradation rate of xenobiotics (Willaert et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Viability of free and encapsulated Escherichia coli overexpressing cyclopentanone monooxygenase monitored during model Baeyer–Villiger biooxidation by confocal laser scanning microscopy

et al. 2011

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Baeyer-Villiger biooxidation of 4-methylcyclohexanone-5-methyloxepane-2-one catalysed by recombinant Escherichia coli overexpressing cyclopentanone monooxygenase encapsulated in polyelectrolyte complex capsules was used to investigate effect of substrate conversion on the viability of cells. Confocal laser scanning microscopy (CLSM) was used to assess cell viability using propidium iodide fluorescence marker for necrosis, and flavin autofluorescence to identify living bacteria. Viability of encapsulated cells decreased with increasing substrate concentration from 99 ± 1 to 83 ± 4%, while substrate conversions from decreased 100 to 6 ± 1%. Storage stabilization of encapsulated cells was observed by increased substrate conversion form 68 ± 2 to 96 ± 3%. Measurements by CLSM with standard deviations up to 5% may be regarded as powerful tool for recombinant cell viability determination during Baeyer-Villiger biooxidations.

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Physiology of Immobilised Microbial Cells

Cited by 6 publications

References 123 publications

A Review of Flavour Formation in Continuous Beer Fermentations*

A Review of Flavour Formation in Continuous Beer Fermentations*

Technologies and Formulation Design of Polysaccharide-Based Hydrogels for Drug Delivery

Viability of free and encapsulated Escherichia coli overexpressing cyclopentanone monooxygenase monitored during model Baeyer–Villiger biooxidation by confocal laser scanning microscopy

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