Physiology of aliphatic hydrocarbon-degrading microorganisms

Watkinson, R. J.; Morgan, Phil G.

doi:10.1007/978-94-011-3452-1_1

Cited by 55 publications

(76 citation statements)

References 53 publications

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“…Alkane hydroxylase initiates the aerobic degradation of alkanes by inserting oxygen atoms at the different sites of alkane terminus (Jauhari et al 2014;Ji et al 2013). Such structures are exemplified by microbodies in alkane-grown bacteria (Watkinson and Morgan 1990). These alkane oxidation enzymes also reported to have diverse substrate ranges or different induction patterns (Hasanuzzaman et al 2007;Meintanis et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

et al. 2017

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Removal of long-chain hydrocarbons and nalkanes from oil-contaminated environments are mere important to reduce the ecological damages, while bioaugmentation is a very promising technology that requires highly efficient microbes. In present study, the efficiency of pure isolates, i.e., Geobacillus thermoparaffinivorans IR2, Geobacillus stearothermophillus IR4 and Bacillus licheniformis MN6 and mixed consortium on degradation of long-chain n-alkanes C 32 and C 40 was investigated by batch cultivation test. Biodegradation efficiencies were found high for C 32 by mixed consortium (90%) than pure strains, while the pure strains were better in degradation of C 40 than mixed consortium (87%). In contrast, the maximum alkane hydroxylase activities (161 lmol mg -1 protein) were recorded in mixed consortium system that had supplied with C 40 as sole carbon source. Also, the alcohol dehydrogenase (71 lmol mg -1 protein) and lipase activity (57 lmol mg -1 protein) were found high. Along with the enzyme activities, the hydrophobicity natures of the bacterial strains were found to determine the degradation efficiency of the hydrocarbons. Thus, the study suggested that the hydrophobicity of the bacteria is a critical parameter to understand the biodegradation of n-alkanes.

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Section: Discussionmentioning

confidence: 99%

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

et al. 2017

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“…Linear and isoprenoid alkanes with neighbouring retention times in the GC chromatogram (e.g., n-C 17 and pristane) have the same physico-chernical properties (e.g., solubility, octanol-water partitioning) and thus should show a similar transport behaviour. However, i-alkanes are less biodegradable due to steric effects (Watkinson and Morgan, 1990). The decrease of the ratio of n-alkanes to i-alkanes is therefore a strong indication for that n-alkanes are rnineralized rather than transported (Pritchard and Costa, 1991).…”

Section: Coupling Of Oxidant Consumption With Hydrocarbon Mineralizationmentioning

confidence: 99%

“…In the aerobic degradation of hydrocarbons, 0 2 plays a dual role: lt is a co-substrate in initial transformation reactions by oxygenases (Watkinson and Morgan, 1990) and it also serves as the final electron acceptor for mineralization. Facultative denitrifying microorganisms can substitute N0 3 and even N0 2 or N 2 0 for 0 2 as terminal electron acceptor (Tiedje, 1988).…”

Section: Role Of Oxidantsmentioning

confidence: 99%

Bioremediation of a diesel fuel contaminated aquifer: simulation studies in laboratory aquifer columns

Hess

Höhener

Hunkeler

et al. 1996

Journal of Contaminant Hydrology

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The in situ bioremediation of aquifers contaminated with petroleum hydrocarbons is commonly based on the infiltration of groundwater supplemented with oxidants (e.g., 0 2 , N0 3 ) and nutrients (e.g., NH1, Pol-). These additions stimulate the microbial activity in the aquifer and several field studies describing the resulting processes have been published. However, due to the heterogeneity of the subsurface and due to the limited number of observation wells usually available, these field data do not offer a sufficient spatial and temporal resolution. In this study, flow-through columns of 47-cm length equipped with 17 sampling ports were filled with homogeneously contaminated aquifer material from a diesel fuel contaminated in situ bioremedia tion site. The columns were operated over 96 days at 12°C with artificial groundwater supple mented with 0 2 , N0 3 and POJ-. Concentration profiles of 0 2 , N0 3 , N0 2 , dissolved inorganic and organic carbon (DIC and DOC, respectively), protein, microbial cells and total residual hydrocarbons were measured. Within the first 12 cm, corresponding to a mean groundwater residence time of < 3.6 h, a steep 0 2 decrease from 4.6 to < 0.3 mg 1-1 , denitrification, a production of DIC and DOC, high microbial cell numbers and a high removal of hydrocarbons were observed. Within a distance of 24 to 40.5 cm from the infiltration, 0 2 was below 0.1 mg 1-1 and a denitrifying activity was found. In the presence and in the absence of 0 2 , n-alkanes were preferentially degraded compared to branched alkanes. The results demonstrate that: (1) infiltra tion of aerobic groundwater into columns filled with aquifer material contaminated with hydrocar bons leads to a rapid depletion of 0 2 ; (2) 0 2 and N0 3 can serve as oxidants for the mineralization of hydrocarbons; and (3) the modelling of redox processes in aquifers has to consider denitrifying activity in presence of 0 2 . ' Corresponding author.

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“…The C 17 :pristane and C 18 :phytane ratios were generally characterized by lower values than the (Watkinson & Morgan 1990). Thus, its disappearance from the culture medium demonstrates the occurrence of biodegradation.…”

Section: Discussionmentioning

confidence: 91%

Diesel oil and PCB-degrading psychrotrophic bacteria isolated from Antarctic seawaters (Terra Nova Bay, Ross Sea)

et al. 2004

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Physiology of aliphatic hydrocarbon-degrading microorganisms

Cited by 55 publications

References 53 publications

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

Bioremediation of a diesel fuel contaminated aquifer: simulation studies in laboratory aquifer columns

Diesel oil and PCB-degrading psychrotrophic bacteria isolated from Antarctic seawaters (Terra Nova Bay, Ross Sea)

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