Physiology of a Bacteria-Free Culture of Trichomonas vaginalis. IV. Effect of Hydrogen Ion Concentration and Oxygen Tension on Population

The effects of environment on Tritrichomonas augusta (Alexeieff, 1911)

“…medium (Table 8) 9.8 5%-6 9.5 6-6% 10.7 6%-7 13.4 7-7% 37.5 7%-8 59.9 35^C 0-4 4-4% 6.0 4%.5 7.6 5-5% 12.0 5%-6 19.8 6-6% 20.0 Hence, the present study indicates for the first time that T. augusta is capable of growing and multiplying in both Diamond's and trichosel media covering a wide pH range. In the current survey it was also found that this flagellate could multiply and undergo morphological changes at lower partial pressures of oxygen similar to those described by Johnson (1942) for Trichomonas vaginalis.…”

“…and 150 mmHg. (control), Johnson (1942), in working with T. vaginalis, reported that the presence of oxygen was definitely unfavorable for growth and division of this flagellate.…”

“…According to this investigator, the change in the pH value toward the acidity caused the death of the organisms while the maximal growth was obtained with constant pH value of 7.55, the approximate pH value of the rectal con tent of the frog. On T. vaginalis Johnson (1942) reported the max imal population in a medium with the pH value between 5.1 and 6.0 and growth practically stopped at pH 7.55 and above.…”

“…According to Johnson (1942), T. vaginalis behaved like a facultative aerobe, and the optimum condition was found to be complete anaerobiosis.…”

“…However, Twohy and Tucker (1961) were able to establish a culture of T. augusta using a modified Diamond's medium. Johnson (1942) subjected the cultures of T. vaginalis to various oxygen tensions. He concluded that the presence of oxygen was def initely unfavorable for multiplication of this organism.…”