Physiology Characterization of Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae on Different Carbohydrate Sources

Abstract

Insect pathogens that are often used to control insect pests are fungi Beauveria bassiana and Metarhizium anisopliae. One of the agar media, which is often used in the entomopathogenic function of propagation in the laboratory, is PDA (potato dextrose agar). The price of instant PDA is quite high, starting from Rp. 750,000 per 250 g doing the research costs quite large. Cheap and easily obtained abundant natural resources can be used as an alternative medium for microorganism growth to reduce the overall costs to be incurred in research. Carbohydrate sources used in this study were potatoes, cassava, sweet potatoes, corn, and rice, which were made into the agar media. Observation parameters were viability rates, colony growth rates, and sporulation of B. bassiana and M. anisopliae fungi isolates. The experiments are used in a completely randomized design (CRD) with two treatment factors. The data obtained were processed with variance and continued with the DNMRT test at 5% significance level. The results showed that cassava agar caused the highest germination at 24 hours. The highest diameter was fungi grown on potato dextrose agar. The highest sporulation was fungi grown on corn agar.

Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores

“…De cada microorganismo se sembraron 100 μL en los medios de cultivo específicos y las cajas de Petri inoculadas se incubaron a 25 ± 2 °C durante 18 y 24 h. En cada tiempo de evaluación se realizó el recuento de conidios germinados y no germinados mediante observación al microscopio con el objetivo 40×. Un conidio se consideró germinado cuando el tubo germinal era igual o mayor a la longitud del mismo conidio (Permadi et al, 2020). El porcentaje de germinación se calculó de acuerdo con la ecuación [1] (Santos Díaz et al, 2022).…”

INITIAL CHARACTERISATION OF Metarhizium anisopliae CPMa1502 FOR THE DEVELOPMENT OF A BIOPESTICIDE AGAINST THE OIL PALM FRUIT SCRAPER Demotispa neivai (Coleoptera: Chrysomelidae)

“…Yeast extract has been identified as a sporulation inductor, and enables increased biomass production due to its vitamin B content (Patil et al, 2014). Also, the starches used in these media can also induce the enzymatic capacity for the degradation of polysaccharides, similar to insect cuticles, and activate several virulence mechanisms (Clarkson and Charnley, 1996;Permadi et al, 2020). The yields achieved were 10 times higher than the values reported for Metarhizium sp., using YM agar (7.7x10 6 conidia cm -2 ) (Aguirre et al, 2009).…”

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

“…The radial growth and conidial germination were used as parameters of physiological characterization of the Metarhizium isolates (Permandi et al 2020). To evaluate the radial growth rate (RGR), 5 μL of a conidial suspension of 1 × 10 6 conidia mL −1 of each isolate was inoculated individually in the center of a Petri dish with SDA.…”