Physiology and whole-plant carbon partitioning during stem sugar accumulation in sweet dwarf sorghum

Babst, Benjamin A.; Karve, Abhijit; Sementilli, Anthony; Dweikat, İsmail; Braun, David

doi:10.1007/s00425-021-03718-w

Cited by 10 publications

(8 citation statements)

References 58 publications

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“…Therefore, increased sucrose accumulation in stems likely results from elevated phloem unloading accompanied by enhanced vacuole storage. Our findings support the conclusion proposed by Babst et al. (2021) , where sugar accumulation in sweet sorghum is driven by elevated phloem unloading and storage in mature stem internodes rather than by increased photoassimilate export in leaves ( Babst et al., 2021 ).…”

Section: Resultssupporting

confidence: 91%

“…Our findings support the conclusion proposed by Babst et al. (2021) , where sugar accumulation in sweet sorghum is driven by elevated phloem unloading and storage in mature stem internodes rather than by increased photoassimilate export in leaves ( Babst et al., 2021 ).…”

Section: Resultssupporting

confidence: 91%

“…Panicles and stems are the major sink tissues importing the photoassimilates after anthesis in grain and sweet sorghum, respectively ( Babst et al., 2021 ). There are no increased photoassimilates produced in source leaves ( Figure S1 ), while the sucrose content in stems increases, likely as a result of removal of the competitive panicles.…”

Section: Discussionmentioning

confidence: 99%

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Sugar accumulation enhancement in sorghum stem is associated with reduced reproductive sink strength and increased phloem unloading activity

Xue,

Beuchat,

Wang

et al. 2023

Front. Plant Sci.

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Sweet sorghum has emerged as a promising source of bioenergy mainly due to its high biomass and high soluble sugar yield in stems. Studies have shown that loss-of-function Dry locus alleles have been selected during sweet sorghum domestication, and decapitation can further boost sugar accumulation in sweet sorghum, indicating that the potential for improving sugar yields is yet to be fully realized. To maximize sugar accumulation, it is essential to gain a better understanding of the mechanism underlying the massive accumulation of soluble sugars in sweet sorghum stems in addition to the Dry locus. We performed a transcriptomic analysis upon decapitation of near-isogenic lines for mutant (d, juicy stems, and green leaf midrib) and functional (D, dry stems and white leaf midrib) alleles at the Dry locus. Our analysis revealed that decapitation suppressed photosynthesis in leaves, but accelerated starch metabolic processes in stems. SbbHLH093 negatively correlates with sugar levels supported by genotypes (DD vs. dd), treatments (control vs. decapitation), and developmental stages post anthesis (3d vs.10d). D locus gene SbNAC074A and other programmed cell death-related genes were downregulated by decapitation, while sugar transporter-encoding gene SbSWEET1A was induced. Both SbSWEET1A and Invertase 5 were detected in phloem companion cells by RNA in situ assay. Loss of the SbbHLH093 homolog, AtbHLH093, in Arabidopsis led to a sugar accumulation increase. This study provides new insights into sugar accumulation enhancement in bioenergy crops, which can be potentially achieved by reducing reproductive sink strength and enhancing phloem unloading.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Sugar accumulation enhancement in sorghum stem is associated with reduced reproductive sink strength and increased phloem unloading activity

Xue,

Beuchat,

Wang

et al. 2023

Front. Plant Sci.

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“…SUT, SWEET, and NAC2–TF are important gene families controlling internode development in sorghum (Mizuno, Kasuga and Kawahigashi, 2016; Zhang et al ., 2018). Past studies reported six SUT genes in sorghum with abundant gene expression in the stem internodes of sweet sorghum cultivar than that of grain one (Li et al ., 2014; Babst et al ., 2021). The SUT4 is reported with putative deletions in Rio (Cooper et al ., 2019); our de novo transcriptome assembly analysis with 223 RNA-seq accessions reported its presence, suggesting that SUT4 is present in the sweet sorghum population.…”

Section: Discussionmentioning

confidence: 99%

Unlocking the Genetic Landscape: Enhanced Insights into Sweet Sorghum Genomes through Comprehensive superTranscriptomic Analysis

Nikhil,

Mohideen,

Raja

2023

Preprint

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Sweet sorghum has gained global significance as a versatile crop for food, fodder, and biofuel. Department of Agriculture, USA declared sorghum a sweet alternative for corn and sugarcane. Its cultivated varieties, along with their wild counterparts, contribute to the core genetic pool. We harnessed 223 publicly available RNA-seq datasets from sweet sorghum to construct the superTranscriptome and analyze gene structure. This approach yielded 45,864 Representative Transcript Assemblies (RTA) that showcased intriguing Presence-Absence Variation (PAV) across 15 existing sorghum genomes, even incorporating one wild progenitor. A fascinating outcome was the identification of 301 superTranscripts exclusive to sweet sorghum, encompassing elements such as hexokinases, cytochromes, select lncRNAs, and histones. Moreover, this study enriched sweet sorghum annotations with 2,802 newly identified protein-coding genes, including 559 encoding diverse transcription factors (TFs). This study unveiled 10,059 superTranscripts associated with various non-coding RNAs, including long non-coding RNAs (lncRNAs). The Rio variety displayed elevated expression of light-harvesting complexes (LHCs) and reduced expression of Metallothioneins during internode growth, suggesting the influence of photosynthesis and metal ion transport on sugar accumulation. Intriguingly, specific lncRNAs exhibited significant expression shifts in Rio during internode development, possibly implying their role in sugar accumulation. We validated the superTranscriptome against the Sweet Sorghum Reference Genome (SSRG) using Differential Exon Usage (DEU) and Differential Gene Expression (DGE), which demonstrated superior estimations. This study underscores the superTranscriptome's utility in unraveling fundamental sorghum mechanisms, enhancing genome annotations, and offering a potential alternative to the reference genome.

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“…GmSUT4 mediated sucrose metabolism and transport SUT, a member of the MFS family, is ubiquitous in plants. Currently, there are many reports of this family gene in various plant species, including monocotyledonous plants such as rise Oryza sativa (Aoki, et al 2003), maize (Zea mays L) (Bezrutczyk, et al 2018), sorghum (Sorghum bicolor (L.)) (Babst, et al 2021), sugarcane (Saccharum o cinarum) (Reinders, et al 2006), and dicotyledons such as Arabidopsis (Meyer, et al 2004;Wippel and Sauer 2012;, cotton (Gossypium sp.) (Yadav, et al 2022), potato (Solanum tuberosum L.) .…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of a soybean GmSUT4 gene reveals its involvement in plant growth and development regulation through sugar metabolism

Chen

Jiao

et al. 2022

Preprint

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Physiology and whole-plant carbon partitioning during stem sugar accumulation in sweet dwarf sorghum

Cited by 10 publications

References 58 publications

Sugar accumulation enhancement in sorghum stem is associated with reduced reproductive sink strength and increased phloem unloading activity

Sugar accumulation enhancement in sorghum stem is associated with reduced reproductive sink strength and increased phloem unloading activity

Unlocking the Genetic Landscape: Enhanced Insights into Sweet Sorghum Genomes through Comprehensive superTranscriptomic Analysis

Functional characterization of a soybean GmSUT4 gene reveals its involvement in plant growth and development regulation through sugar metabolism

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