Physiology and transcriptomic analysis of endogenous hormones regulating in vitro adventitious root formation in tree peony

Wen, Shu-Sheng; Miao, Dapeng; Cui, Huiying; Li, Shenghao; Gu, Yu; Jia, Ruirui; Leng, Yifan

doi:10.1016/j.scienta.2023.112122

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…Previous studies have highlighted the requirement for higher levels of endogenous IAA to induce the formation of root primordia during adventitious root development. However, the IAA content decreased during the growth and differentiation of root primordia, indicating a correlation between an increased IAA content and the formation and occurrence of adventitious roots [14][15][16]. This experiment supports the same observation, affirming that IAA serves as a crucial endogenous hormone in the induction of adventitious root formation in mulberry cuttings.…”

Section: Growth and Development Of Adventitious Rootssupporting

confidence: 84%

Physicochemical Response of External Plant Growth Regulator in the Cutting Process of Mulberry

Sun,

Dou,

Chen

et al. 2023

Horticulturae

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Adventitious roots play a crucial role in the nourishment and propagation of arboreal vegetation. In order to shed light on the physiological and biochemical characteristics of the challenging-to-propagate mulberry tree species, an investigation was conducted. This study aimed to compare the responses of various root morphological indicators, endogenous hormones, and oxidase activities in the “Yueshenda 10” fruit mulberry, at different stages of treatment. The ultimate objective was to identify the factors influencing the process of root development. The findings revealed a distinct ”/\“ pattern in the levels of IAA and JA within the cuttings. Conversely, the changes in ABA, ZR, and GA3 exhibited a ”/\/“ pattern. The fluctuation of the IAA/ABA values followed a ”\/\“ mode, whereas the IAA/ZR values initially increased, followed by a subsequent decrease. The correlation between the initial concentrations of these five endogenous hormones and the rooting rate displayed variations. Notably, IAA demonstrated the strongest association with the rooting rate, exhibiting a positive correlation with both IAA and ZR. Regarding the activity of three antioxidant enzymes (IAAO, POD, and PPO), a ”/\“ trend was observed, wherein the enzyme activity increased under ABT1 treatment. However, the peak activity levels of the enzymes appeared during different periods: callus generation, rooting induction, and adventitious root expression, respectively. Overall, the most effective treatment for promoting root development and significantly enhancing the root growth parameters of mulberry was found to be 800 mg/L ABT1. Exogenous hormone treatment expedited the synthesis of antioxidant enzymes, thereby shortening the rooting time and facilitating root formation.

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Section: Growth and Development Of Adventitious Rootssupporting

confidence: 84%

Physicochemical Response of External Plant Growth Regulator in the Cutting Process of Mulberry

Sun,

Dou,

Chen

et al. 2023

Horticulturae

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“…Under in vitro culture conditions, plant hormones and their interactions with exogenous PGRs not only have a determinative effect on explant dedifferentiation and differentiation [23,24], but also can regulate the physiological status and phenotypical characteristics of the cultures [27,29]. During in vitro culture, the aberrant or unexpected phenotypes of the cultured products markedly reduce the regeneration efficiency [26,48].…”

Section: Differences In the Endogenous Hormone Content And Antioxidan...mentioning

confidence: 99%

“…Plant endogenous hormones play an important role in almost all processes of plant growth, development, senescence, and stress responses [23,24]. During plant tissue culture, they are considered to be one of the crucial factors regulating plant organogenesis and morphogenesis [25,26].…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

Zhang,

Yin

et al. 2023

Plants

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The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro plant regeneration from the stem nodes of the Jerusalem artichoke via direct organogenesis is presented. In the shoot induction stage, the stem segments produced more shoots with vigorous growth on MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA). The concentrations of 6-BA and gibberellic acid (GA3) were both optimized at 0.5 mg/L for shoot multiplication, and the combination of 0.05 mg/L indole-3-butyric acid (IBA) and 0.05 mg/L 1-naphthylacetic acid (NAA) was the most responsive for root induction, yielding the largest number of roots. The regenerated plantlets were successfully hardened at a 96% survival rate and vigorously grew in the field. The genetic stability of the regenerated plants was confirmed by flow cytometry and simple sequence repeat (SSR) analysis. However, 17.3% of shoots on the optimum shoot induction medium had withered leaves and excessive callus (atypical shoots), which greatly reduced the induction efficiency. Enzyme activity in the typical and atypical shoots was compared. The atypical shoots had significantly higher levels of endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA), as well as increased activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), whereas the content of 6-BA, zeatin (ZT), and GA3 was significantly reduced. The activity of the three enzymes was positively correlated with the content of IAA and ABA, while being negatively correlated with that of 6-BA, ZT, and GA3. The results suggest that the poor growth of the atypical shoots might be closely related to the significant accumulation of endogenous IAA and ABA, thus significantly increasing antioxidant enzyme activity.

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“…This highlights the significance of antioxidant enzyme-related genes in the development of adventitious roots [21]. In tree peonies, certain genes related to plant hormones were identified through transcriptome analysis, and these genes were found to be key in promoting the formation of root primordia [22,23]. In short, the research has mainly focused on the molecular regulation mechanism of hormones on rooting, but there are few studies on the effects of antioxidant enzyme-related genes on rooting.…”

Section: Introductionmentioning

confidence: 99%

RNA Sequencing Analysis and Verification of Paeonia ostii ‘Fengdan’ CuZn Superoxide Dismutase (PoSOD) Genes in Root Development

Wang,

Song,

Wang

et al. 2024

Plants

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Tree peony (Paeonia suffruticosa) is a significant medicinal plant. However, the low rooting number is a bottleneck problem in the micropropagation protocols of P. ostii ‘Fengdan’. The activity of superoxide dismutase (SOD) is closely related to root development. But research on the SOD gene’s impact on rooting is still lacking. In this study, RNA sequencing (RNA-seq) was used to analyze the four crucial stages of root development in P. ostii ‘Fengdan’ seedlings, including the early root primordium formation stage (Gmfq), root primordium formation stage (Gmf), root protrusion stage (Gtq), and root outgrowth stage (Gzc). A total of 141.77 GB of data were obtained; 71,718, 29,804, and 24,712 differentially expressed genes (DEGs) were identified in the comparison groups of Gmfq vs. Gmf, Gmf vs. Gtq, and Gtq vs. Gzc, respectively. Among the 20 most highly expressed DEGs in the three comparison groups, only the CuZnSOD gene (SUB13202229, PoSOD) was found to be significantly expressed in Gtq vs. Gzc. The overexpression of PoSOD increased the number of adventitious roots and promoted the activities of peroxidase (POD) and SOD in P. ostii ‘Fengdan’. The gene ADVENTITIOUS ROOTING RELATED OXYGENASE1 (PoARRO-1), which is closely associated with the development of adventitious roots, was also significantly upregulated in overexpressing PoSOD plants. Furthermore, PoSOD interacted with PoARRO-1 in yeast two-hybrid (Y2H) and biomolecular luminescence complementation (BiFC) assays. In conclusion, PoSOD could interact with PoARRO-1 and enhance the root development of tube plantlets in P. ostii ‘Fengdan’. This study will help us to preliminarily understand the molecular mechanism of adventitious root formation and improve the root quality of tree peony and other medicinal plants.

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Physiology and transcriptomic analysis of endogenous hormones regulating in vitro adventitious root formation in tree peony

Cited by 9 publications

References 46 publications

Physicochemical Response of External Plant Growth Regulator in the Cutting Process of Mulberry

Physicochemical Response of External Plant Growth Regulator in the Cutting Process of Mulberry

Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

RNA Sequencing Analysis and Verification of Paeonia ostii ‘Fengdan’ CuZn Superoxide Dismutase (PoSOD) Genes in Root Development

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