Physiology and genetics of methylotrophic bacteria

DEVRIES, G; Kües, Ursula; Ståhl, Ulf

doi:10.1016/0378-1097(90)90523-s

Cited by 36 publications

(22 citation statements)

References 113 publications

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“…calcoaceticus contains a PQQ requiring glucose dehydrogenase. At least four genes appear to be involved in PQQ synthesis in this organism (36,37,40). Genes 3 and 4 are located on separate operons.…”

Section: Pqq Synthesismentioning

confidence: 98%

Physiologic Importance of Pyrroloquinoline Quinone

Smidt

Steinberg

Rucker

1991

Experimental Biology and Medicine

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Pyrroloquinoline quinone (PQQ, methoxatin) is a dissociable cofactor for a number of bacterial dehydrogenases. The compound is unusual because of its ability to catalyze redox cycling reactions at a high rate of efficiency and it has the potential of catalyzing various carbonyl amine reactions as well. In methylotrophic bacteria, PQQ is derived from the condensation of L-tyrosine with L-glutamic acid. Whether or not PQQ serves as a cofactor in higher plants and animals remains controversial. Nevertheless, a strong case may be made that PQQ and related quinoids have nutritional and pharmacologic importance. In highly purified, chemically defined diets, PQQ stimulates animal growth. Furthermore, PQQ deprivation appears to impair connective tissue maturation, particularly when initiated in utero and throughout perinatal development.

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Section: Pqq Synthesismentioning

confidence: 98%

Physiologic Importance of Pyrroloquinoline Quinone

Smidt

Steinberg

Rucker

1991

Experimental Biology and Medicine

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“…Urea addition led to 1.35 and 3.16 times more pmoA genes than that in only CH 4 -incubated microcosms on day 5 and day 19, respectively. The family Methylophilaceae, using methanol as sole source of carbon and energy (Bratina et al, 1992;Devries et al,1990;Kolb, 2009), was methanol-oxidizing bacteria analyzed in our study. Similar trend was observed for 16S rRNA gene sequences affiliated with methanol-oxidizing bacteria (Fig.…”

Section: Y Zheng Et Al: Interactions Between Soil Methane and Ammonmentioning

confidence: 99%

“…The known soil-retrieved methanol-oxidizing bacteria are quite diverse; however, most of them are facultative methylotrophic, indicating the capability to utilize alternative carbon substrate (Kolb, 2009). The family Methylophilaceae is the known obligate methylotrophs that use methanol as the sole source of carbon and energy (Bratina et al, 1992;Devries et al, 1990;Kolb, 2009). Nitrifying bacteria use ammonia monooxygenase (AMO) for oxidation of their primary growth substrate.…”

Section: Introductionmentioning

confidence: 99%

Competitive interactions between methane- and ammonia-oxidizing bacteria modulate carbon and nitrogen cycling in paddy soil

et al. 2014

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Abstract.Pure culture studies have demonstrated that methanotrophs and ammonia oxidizers can both carry out the oxidation of methane and ammonia. However, the expected interactions resulting from these similarities are poorly understood, especially in complex, natural environments. Using DNA-based stable isotope probing and pyrosequencing of 16S rRNA and functional genes, we report on biogeochemical and molecular evidence for growth stimulation of methanotrophic communities by ammonium fertilization, and that methane modulates nitrogen cycling by competitive inhibition of nitrifying communities in a rice paddy soil. Pairwise comparison between microcosms amended with CH 4 , CH 4 +Urea, and Urea indicated that urea fertilization stimulated methane oxidation activity 6-fold during a 19-day incubation period, while ammonia oxidation activity was significantly suppressed in the presence of CH 4 . Pyrosequencing of the total 16S rRNA genes revealed that urea amendment resulted in rapid growth of Methylosarcina-like MOB, and nitrifying communities appeared to be partially inhibited by methane. High-throughput sequencing of the 13 Clabeled DNA further revealed that methane amendment resulted in clear growth of Methylosarcina-related MOB while methane plus urea led to an equal increase in Methylosarcina and Methylobacter-related type Ia MOB, indicating the differential growth requirements of representatives of these genera. An increase in 13 C assimilation by microorganisms related to methanol oxidizers clearly indicated carbon transfer from methane oxidation to other soil microbes, which was enhanced by urea addition. The active growth of type Ia methanotrops was significantly stimulated by urea amendment, and the pronounced growth of methanol-oxidizing bacteria occurred in CH 4 -treated microcosms only upon urea amendment. Methane addition partially inhibited the growth of Nitrosospira and Nitrosomonas in urea-amended microcosms, as well as growth of nitrite-oxidizing bacteria. These results suggest that type I methanotrophs can outcompete type II methane oxidizers in nitrogen-rich environments, rendering the interactions among methane and ammonia oxidizers more complicated than previously appreciated.

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“…Difficulties in isolating auxotrophic mutants of methylotrophs have been reviewed by Holloway (1984) and only a small number of such mutants have been described (De Vries et al 1990). Tsygankov and Kazakova (1987) suggested that these problems resulted from a low-affinity uptake system for some nutrients and growth inhibition by combinations of supplements.…”

Section: Discussionmentioning

confidence: 99%

“…not very effective in producing auxotrophic and other phenotypic mutants. The reasons for these difficulties are not yet understood (De Vries et al 1990). Only few mutants of Hyphomicrobium spp.…”

Section: Introductionmentioning

confidence: 97%

Mutagenesis and chromosome mobilization in Hyphomicrobium facilis B-522

Gliesche¹,

Hirsch²

1992

Can. J. Microbiol.

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Spontaneously derived antibiotic-resistant mutants of Hyphomicrobium facilis B-522, a restricted facultative methylotroph, occurred at a high frequency on agar plates with low antibiotic concentrations. Mutants specifically defective in methanol oxidation have been obtained using an allyl alcohol direct selection technique. By chemical mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine in the presence of chloramphenicol several stable auxotrophic mutants could be isolated: three leucine auxotrophs, two threonine auxotrophs, and two leucine-methionine double auxotrophic mutants. Optimal conditions for transposon mutagenesis have been developed by comparing several transposon delivery vectors. With the suicide plasmid pRK2013 as a vector, the tetracycline resistance conferring transposon Tn5-132 was introduced into the genome of H. facilis B-522. The following insertion mutants have been obtained: leu-3::Tn5-132, ilv-1::Tn5-132, and pur-1::Tn5-132. Broad host range IncP-1 plasmids could be successfully transferred by interspecific matings. Chromosome mobilization was demonstrated with the conjugative IncP-1 plasmids RP1, R68.45, pMO60, and H. facilis 2189 (leu-2, met-1, mox-1, nfs-1, str-12) as recipient strain. Transconjugants occurred at frequencies ranging from 10(-6) to 10(-8) for each marker.

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Physiology and genetics of methylotrophic bacteria

Cited by 36 publications

References 113 publications

Physiologic Importance of Pyrroloquinoline Quinone

Physiologic Importance of Pyrroloquinoline Quinone

Competitive interactions between methane- and ammonia-oxidizing bacteria modulate carbon and nitrogen cycling in paddy soil

Mutagenesis and chromosome mobilization in Hyphomicrobium facilis B-522

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