Physiological, ultrastructural and proteomic responses of tobacco seedlings exposed to silver nanoparticles and silver nitrate

Štefanić, Petra Peharec; Cvjetko, Petra; Biba, Renata; Domijan, Ana Marija; Letofsky-Papst, Ilse; Tkalec, Mirta; Šikić, Sandra; Cindrić, Mario; Balen, Biljana

doi:10.1016/j.chemosphere.2018.06.128

Cited by 51 publications

(33 citation statements)

References 94 publications

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“…Because of this, the work carried out could neither confirm nor preclude the presence of NPs in the tissues and cells of leaves or needles, or else stems and roots. It should also be noted that some studies indicate changes in the ultrastructure of cells without the presence of nanoparticles [7]. The results obtained show that changes in the ultrastructure are in fact confined to the assimilatory apparatus, are not major, and only arise with the highest of the concentrations studied (50 ppm), albeit pointing unambiguously to the phytotoxic action of both of the studied NPs.…”

Section: Discussionmentioning

confidence: 55%

“…Our work thus shows that, in woody plants, the cellular organelles most prone to the action of NPs are indeed chloroplasts. Changes of a similar nature have been observed in herbaceous plants as a result of the action of different kinds of NP [7,25,[27][28][29]. Some of the observed changes do not represent a specific reaction to the action of NPs on the part of plants.…”

Section: Discussionmentioning

confidence: 56%

“…They did not induce more major change in cell-ultrastructure, other than in terms of the enlarged size of these vacuoles. It likewise proved impossible to confirm that transport to leaves had taken place [7]. In turn, FeNPs taken in through the roots of Capsicum annuum did prove damaging to vascular bundles where the concentrations involved were greater, while only serving to regulate the development of the bundles where concentrations were lower [27].…”

Section: Discussionmentioning

confidence: 97%

“…Most studies show that NPs 5 nm in size are taken up by both roots and above-ground parts of plants, and transported via both phloem and xylem [6,8,9,12]. However, work by Peharec Štefanić et al [7] failed to confirm this kind of transport. AgNPs proved not to be present in the leaves of tobacco seedlings, even though changes in the ultrastructure of chloroplasts and the presence of the nanoparticles in roots were both detected.…”

Section: Discussionmentioning

confidence: 99%

“…While changes in the ultrastructure of leaves reflecting the application of NPs have already been documented in quite a large number of studies [7,25,[27][28][29], work involving the ultrastructure of stems and roots has been rare. AgNPs taken in via cells of tobacco-plant roots were most often accumulated in vacuoles.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Copper and Silver Nanoparticles on Container-Grown Scots Pine (Pinus sylvestris L.) and Pedunculate Oak (Quercus robur L.) Seedlings

Aleksandrowicz-Trzcińska

Bederska-Błaszczyk

Szaniawski

et al. 2019

Forests

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Metal nanoparticles (NPs) are finding ever-wider applications in plant production (agricultural and forestry-related) as fertilisers, pesticides and growth stimulators. This makes it essential to examine their impact on a variety of plants, including trees. In the study detailed here, we investigated the effects of nanoparticles of silver and copper (i.e., AgNPs and CuNPs) on growth, and chlorophyll fluorescence, in the seedlings of Scots pine and pedunculate oak. We also compared the ultrastructure of needles, leaves, shoots and roots of treated and untreated plants, under transmission electron microscopy. Seedlings were grown in containers in a peat substrate, prior to the foliar application of NPs four times in the course of the growing season, at the four concentrations of 0, 5, 25 and 50 ppm. We were able to detect species-specific activity of the two types of NP. Among seedling pines, the impact of both types of NP at the concentrations supplied limited growth slightly. In contrast, no such effect was observed for the oaks grown in the trial. Equally, it was not possible to find ultrastructural changes in stems and roots associated with the applications of NPs. Cell organelles apparently sensitive to the action of both NPs (albeit only at the highest applied concentration of 50 ppm) were chloroplasts. The CuNP-treated oaks contained large plastoglobules, whereas those dosed with AgNP contained large starch granules. The NP-treated pines likewise exhibited large numbers of plastoglobules, while the chloroplasts of NP-treated plants in general presented shapes that changed from lenticular to round. In addition, large osmophilic globules were present in the cytoplasm. Reference to maximum quantum yields from photosystem II (Fv/Fm)—on the basis of chlorophyll a fluorescence measurements—revealed a slight debilitation of oak seedlings following the application of both kinds of NP at higher concentrations. In contrast, in pines, this variable revealed no influence of AgNPs, as well as a favourable effect due to the CuNPs applied at a concentration of 5 ppm. Our research also showed that any toxic impact on pine or oak seedlings due to the NPs was limited and only present with higher concentrations.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The Effects of Copper and Silver Nanoparticles on Container-Grown Scots Pine (Pinus sylvestris L.) and Pedunculate Oak (Quercus robur L.) Seedlings

Aleksandrowicz-Trzcińska

Bederska-Błaszczyk

Szaniawski

et al. 2019

Forests

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Designer nanoparticles for plant cell culture systems: Mechanisms of elicitation and harnessing of specialized metabolites

et al. 2021

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Plant cell culture systems have become an attractive and sustainable approach to produce high-value and commercially significant metabolites under controlled conditions. Strategies involving elicitor supplementation into plant cell culture media are employed to mimic natural conditions for increasing the metabolite yield. Studies on nanoparticles (NPs) that have investigated elicitation of specialized metabolism have shown the potential of NPs to be a substitute for biotic elicitors such as phytohormones and microbial extracts. Customizable physicochemical characteristics allow the design of monodispersed-, stimulus-responsive-, and hormone-carrying-NPs of precise geometries to enhance their elicitation capabilities based on target metabolite/plant cell culture type. We contextualize advances in NP-mediated elicitation, especially stimulation of specialized metabolic pathways, the underlying mechanisms, impacts on gene regulation, and NP-associated cytotoxicity. The novelty of the concept lies in unleashing the potential of designer NPs to enhance yield, harness metabolites, and transform nanoelicitation from exploratory investigations to a commercially viable strategy.

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Proteomic evaluation of nanotoxicity in aquatic organisms: A review

2022

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The alteration of organisms protein functions by engineered nanoparticles (ENPs) is dependent on the complex interplay between their inherent physicochemical properties (e.g., size, surface coating, shape) and environmental conditions (e.g., pH, organic matter). To date, there is increasing interest on the use of 'omics' approaches, such as proteomics, genomics, and others, to study ENPs-biomolecules interactions in aquatic organisms. However, although proteomics has recently been applied to investigate effects of ENPs and associated mechanisms in aquatic organisms, its use remain limited. Herein, proteomics techniques widely applied to investigate ENPs-protein interactions in aquatic organisms are reviewed. Data demonstrates that 2DE and mass spectrometry and/or their combination, thereof, are the most suitable techniques to elucidate ENPs-protein interactions. Furthermore, current status on ENPs and protein interactions, and possible mechanisms of nanotoxicity with emphasis on those that exert influence at protein expression levels, and key influencing factors on ENPs-proteins interactions are outlined. Most reported studies were done using synthetic media and essay protocols and had wide variability (not standardized); this may consequently limit data application in actual environmental systems. Therefore, there is a need for studies using realistic environmental concentrations of ENPs, and actual environmental matrixes (e.g., surface water) to aid better model development of ENPs-proteins interactions in aquatic systems.

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Physiological, ultrastructural and proteomic responses of tobacco seedlings exposed to silver nanoparticles and silver nitrate

Cited by 51 publications

References 94 publications

The Effects of Copper and Silver Nanoparticles on Container-Grown Scots Pine (Pinus sylvestris L.) and Pedunculate Oak (Quercus robur L.) Seedlings

The Effects of Copper and Silver Nanoparticles on Container-Grown Scots Pine (Pinus sylvestris L.) and Pedunculate Oak (Quercus robur L.) Seedlings

Designer nanoparticles for plant cell culture systems: Mechanisms of elicitation and harnessing of specialized metabolites

Proteomic evaluation of nanotoxicity in aquatic organisms: A review

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