Physiological, Transcriptomic Investigation on the Tea Plant Growth and Yield Motivation by Chitosan Oligosaccharides

Abstract

Chitosan oligosaccharides (COS) has been abundantly studied for its application on regulating plant growth of many horticultural and agricultural crops. We presented here the effect of COS on tea plant growth and yield by physiological and transcriptomic checking. The results showed that COS treatment can enhance the antioxidant activity of superoxide dismutase (SOD) and peroxidase (POD) and increase the content of chlorophyll and soluble sugar in tea plants. The field trail results show that COS treatment can increase tea buds’ density by 13.81–23.16%, the weight of 100 buds by 15.94–18.15%, and the yield by 14.22–21.08%. Transcriptome analysis found 5409 COS-responsive differentially expressed genes (DEGs), including 3149 up-regulated and 2260 down-regulated genes, and concluded the possible metabolism pathway that responsible for COS promoting tea plant growth. Our results provided fundamental information for better understanding the molecular mechanisms for COS’s acting on tea plant growth and yield promotion and offer academic support for its practical application in tea plant.

Transcriptomic analysis of pea plant responses to chitooligosaccharides’ treatment revealed stimulation of mitogen-activated protein kinase cascade

“…Taken together, our results point to a rapid and significant activation of pea genes involved in regulation of host defense responses, induced by CO8-DA, in accordance with previous findings for chitin-related compounds in Arabidopsis, rice, potato, tea (Chen et al, 2020;Lemke et al, 2020;Siriwong et al, 2021;Ichimaru et al, 2022;Ou et al, 2022). We have shown that a set of genes encoding specific enzymes involved in the biosynthesis of phytoalexins and other secondary metabolites was up-regulated in response to CO8-DA, that was in line with previous findings in other plants (Vasyukova et al, 2001;Khan et al, 2003;Zhang et al, 2020).…”

“…The pea plant treatment with CO8-DA stimulated the synthesis of such components as lignins, reinforcing the cell wall. Similar effect of COs was previously shown in rice and tea ( Siriwong et al., 2021 ; Ou et al., 2022 ).…”

Chitooligosaccharide Maintained Cell Membrane Integrity by Regulating Reactive Oxygen Species Homeostasis at Wounds of Potato Tubers during Healing

“…It has been reported that COS induced extracellular Ca 2+ influx and increased the resistance of tobacco against the tobacco mosaic virus [ 32 ]. Moreover, COS increased the activity of calcium-dependent protein kinase (CDPK) in Brassica napus [ 33 ] and up-regulated the expression of CDPK7 and CDPK26 in Arabidopsis [ 34 ]. Increased concentration of intracellular Ca 2+ activated CDPK, and the interaction of CDPK and NOX induced ROS bursts at tuber wounds [ 3 ].…”

Chitosan oligosaccharide as a plant immune inducer on the Passiflora spp. (passion fruit) CMV disease

“…Li et al (2022b) found that the application of physcion and COS combination is more effective in facilitating the SOD, POD activities of the maize plants, resulting in plants with high stress resistance. Ou et al (2022) reported that COS could enhance the antioxidant activity of SOD and POD in tea plants.…”