Physiological role of alternative oxidase (from yeasts to plants)

Рогов, А. Г.; Zvyagilskaya, R. A.

doi:10.1134/s0006297915040021

Cited by 44 publications

(34 citation statements)

References 96 publications

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“…This report improves our understanding of the protective role played by Aox in plants, fungi and some protozoa against the most diverse experimental stresses [25–26]. …”

Section: Discussionmentioning

confidence: 77%

High Osmolarity Environments Activate the Mitochondrial Alternative Oxidase in Debaryomyces Hansenii

et al. 2017

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The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical orders, has been related to different stress responses. However, little is known about its role in mitochondria during transitions from low to high saline environments. In this report, we analyze the effects of Aox in shifts from low to high salt concentrations in the culture media. At early stages of a salt insult, we observed that this protein prevents the overflow of electrons on the mitochondrial respiratory chain, thus, decreasing the production of reactive oxygen species. Interestingly, in the presence of high osmolite concentrations, Aox activity is able to sustain a stable membrane potential when coupled to complex I, despite a compromised cytochrome pathway. Taken together, our results suggest that under high osmolarity conditions Aox plays a critical role regulating mitochondrial physiology.

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“…This report improves our understanding of the protective role played by Aox in plants, fungi and some protozoa against the most diverse experimental stresses [25–26]. …”

Section: Discussionmentioning

confidence: 77%

High Osmolarity Environments Activate the Mitochondrial Alternative Oxidase in Debaryomyces Hansenii

et al. 2017

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“…Mitochondria of most organisms studied so far contain, along with the classical phosphorylating cytochrome pathway, a so‐called cyanide‐insensitive alternative oxidase (AOX), localized on the matrix side of the mitochondrial inner membrane (Rogov and Zvyagilskaya, ). It has been demonstrated that Aspergillus fumigatus produces an AOX that contributes to both reductions of ROS generated within mitochondria and regulation of energy production and metabolism (Hattori et al, ).…”

Section: Resultsmentioning

confidence: 99%

Analysis ofParacoccidioideslutziimitochondria: a proteomic approach

Casaletti

Lima

Oliveira

et al. 2017

Yeast

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The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MS system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd.

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“…An alternative oxidase system is found in all plants, most fungi, algae, and some protists (Rogov and Zvyagilskaya, 2015). Aox is located in the inner mitochondrial membrane where it receives electrons from reduced ubiquinone and catalyzes the reduction of oxygen to water.…”

Section: Introductionmentioning

confidence: 99%

Alternative oxidase induction protects Candida albicans from respiratory stress and promotes hyphal growth

Duvenage

Walker

Bojarczuk

et al. 2018

Preprint

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21The human fungal pathogen Candida albicans possesses two genes expressing a 22 cyanide-insensitive Alternative Oxidase (Aox) enzymes in addition to classical and 23 parallel electron transfer chains (ETC). In this study, we examine the role of Aox in C. 24 albicans under conditions of respiratory stress, which may be inflicted during its 25 interaction with the human host or co-colonising bacteria. We find that the level of Aox 26 expression is sufficient to modulate resistance to classical ETC inhibition under 27 respiratory stress and are linked to gene expression changes that can promote both 28 survival and pathogenicity. For example we demonstrate that Aox function is important 29 for the regulation of filamentation in C. albicans and observe that cells lacking Aox 30 function lose virulence in a zebrafish infection model. Our investigations also identify 31 that pyocyanin, a phenazine produced by the co-colonising bacterium 32 Pseudomonas aeruginosa, inhibits Aox-based respiration in C. albicans. These results 33 suggest that Aox plays important roles within respiratory stress response pathways 34 which C. albicans may utilise both as a commensal organism and as a pathogen. 35 36 37C. albicans is a commensal yeast which is found in the majority of the population. 38

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Physiological role of alternative oxidase (from yeasts to plants)

Cited by 44 publications

References 96 publications

High Osmolarity Environments Activate the Mitochondrial Alternative Oxidase in Debaryomyces Hansenii

High Osmolarity Environments Activate the Mitochondrial Alternative Oxidase in Debaryomyces Hansenii

Analysis ofParacoccidioideslutziimitochondria: a proteomic approach

Alternative oxidase induction protects Candida albicans from respiratory stress and promotes hyphal growth

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