Physiological responses of lambari<i>Astyanax altiparanae</i>(Garutti &amp; Britski 2000) to air exposure

Pereira-da-Silva, Elyara Maria; Oliveira, Ricardo Henrique Franco de

doi:10.1111/are.13018

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…After acclimation (15 days) fish were sorted to one of four treatments (20 fish per treatment), in a completely randomized experimental design: control – C (without anaesthesia or stress), anaesthesia – A (deep anaesthesia induced by 50 mg/L clove oil), stress – S (5 min air exposure) and pre‐anaesthesia associated to stress A + S (deep anaesthesia induced by clove oil prior the 5 min air exposure). The induction to deep anaesthesia was conducted as described previously for the same species and the fish were stressed by 5‐min air exposure (Pereira‐da‐Silva & Oliveira, ; Pereira‐da‐Silva et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Fish receiving treatment A (anaesthesia) were individually captured and anaesthetized (deep anaesthesia) with clove oil diluted in water at a concentration of 50 mg/L and then transferred to their experimental units, where they remained for 30 min (Pereira‐da‐Silva & Oliveira, ) and the same procedures described for the control group were performed, submitting the fish only to a light sedation with clove oil for biometry and blood collection (Pereira‐da‐Silva et al, ). Fish receiving treatment S (stress) were captured and immediately subjected to an acute stressor (5 min air exposure) (Pereira‐da‐Silva & Oliveira, ; Pereira‐da‐Silva, Oliveira, & Del Nero, ) and fish receiving treatment A + S (anaesthesia prior to stress) were anaesthetized (deep anaesthesia) before exposure to air. In these treatments, fish were also placed back into their experimental units where they were kept for 30 min prior to tissue collection for the same assays previously described.…”

Section: Methodsmentioning

confidence: 99%

“…Air exposure is a common acute stressor that occurs during handling in fish farming, laboratory treatment or even in sport fishing (catch and release) that causes changes in osmolarity, reduction of liver glycogen stores and significant elevations in cortisol, glucose, lactate, proteins and plasma triglycerides (Acerete, Balasch, Espinosa, Josa, & Tort, ; Arends, Mancera, Muñoz, Wendelaar Bonga, & Flik, ; Luz & Portella, ). This practice also restricts water flow through the gills and fish body, reduces body hydration and the oxygen supply in the gills, resulting in damages, oxidative stress and elevation of lipid peroxidation which can negatively affect the quality of meat and its by‐products (Martínez‐Álvarez et al, ; Min & Ahn, ; Ramsay et al, ; Velisek et al, ; Vinagre, Madeira, Narciso, Cabral, & Diniz, ; Paital, ; Pereira‐da‐Silva & Oliveira, ).…”

Section: Introductionmentioning

confidence: 99%

“…stricts water flow through the gills and fish body, reduces body hydration and the oxygen supply in the gills, resulting in damages, oxidative stress and elevation of lipid peroxidation which can negatively affect the quality of meat and its by-products (Martínez-Álvarez et al, 2005;Min & Ahn, 2005;Ramsay et al, 2009;Velisek et al, 2011;Vinagre, Madeira, Narciso, Cabral, & Diniz, 2012;Paital, 2013;Pereira-da-Silva & Oliveira, 2017).…”

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confidence: 99%

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Clove oil attenuates stress responses in lambari,Astyanax altiparanae

2019

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The effects of clove oil anaesthetic on mitigating the physiological responses to air exposure, a stressful and routine situation in fish farming, laboratory conditions and sport fishing (catch and release), were evaluated in lambari (A. altiparanae). Adult females (n = 80) were randomly sorted to receive one of four treatments: control, anaesthesia (clove oil 50 mg/L), stress (5 min air exposure) and pre‐anaesthesia associated to stress. Their cortisol, glucose, lactate and haematocrit levels, the hepatosomatic index (HSI), liver and muscle glycogen, lipid peroxidation and the enzymatic activity of lactate dehydrogenase (LDH), catalase (CAT) and glutathione reductase (GR), were recorded. Glucose levels increased (53.9%) after anaesthesia and/or stress. The stress situation increased plasma cortisol (146.6%), lactate (294.6%) and lipid peroxidation in white muscle (45%) and decreased glycogen in white muscle (40.1%). The haematocrit increased after stress or anaesthesia (7.9%) while the liver glycogen and HSI did not change. Anaesthesia or stress did not affect the LDH but reduced the activity of CAT (46.1%) and GR (30.3%). We concluded that the anaesthetic clove oil in the concentration 50 mg/L modulates the physiological responses to air exposure stress improving the welfare; air exposure and clove oil affect the antioxidant defences of lambari; the activity of CAT and GR and the concentration of MDA can be used as biomarkers of stress in A. altiparanae.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Clove oil attenuates stress responses in lambari,Astyanax altiparanae

2019

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“…In the ovary, vitellogenin is captured by receptor‐mediated endocytosis and processed into several other components of the yolk (Yilmaz et al., ). There are many studies in the past decades that have evaluated the contribution of the liver to oogenesis through variation of biological indices, such as the hepatosomatic index (Alvarenga, Bazzoli, Santos, & Rizzo, ; Hauser‐Davis, Oliveira, Silveira, Silva, & Ziolli, ; Pereira‐da‐Silva & Oliveira, );. These indices measure the relationship between the weight of the organ and the individual.…”

Section: Introductionmentioning

confidence: 99%

Oogenesis is accompanied by cyclic morphological changes in hepatocytes of Neotropical freshwater fish Piabina argentea

Thomé

Cardoso

Oliveira

et al. 2018

Anat Histol Embryol

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We determined for the first time the reproductive biology of Piabina argentea through macroscopic and microscopic analysis of ovaries and evaluated the morphological changes in hepatocytes. Two hundred and 46 specimens were collected, 204 females and 42 males, between March 2014 and February 2015. Biometrics data were obtained. From females, gonad and liver samples were conducted to histological, histochemical and immunohistochemical techniques. Mature ovaries were used to determine absolute and relative fecundity. Total length and body weight values indicated that females were larger than males. The estimated weight-length ratio showed negative allometric growth. The absolute fecundity average was 171.83 ± 59.89 oocytes per ovary. In addition, females spawning capable and regressing stages were found throughout the sampling period and the presence of all oocyte types in regressing stage ovaries indicated asynchronous oocyte development and multiple spawning. From regenerating to spawning capable stage the oocytes accumulated yolk in cytoplasm became bigger. While in the liver hepatocytes with a larger cell area during regenerating stage and proliferative activity in the spawning capable stage were observed. Thus, our results indicate that P. argentea had an opportunistic reproductive strategy and cyclic morphological changes of hepatocytes occurred during the oogenesis.

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Vitamin C combined with oregano essential oil, Origanum vulgare, reduced oxidative damage in Astyanax aff. bimaculatus exposed to air

et al. 2023

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The dietary impacts of water-soluble (vitamin C) and fat-soluble (oregano essential oil, OEO) antioxidants on the stress responses and redox status of Astyanax aff. bimaculatus exposed to the air were assessed. Five diets with vitamin C (VC 0, VC 40, VC 80, VC 120, and VC 160 mg kg − 1 ) with or without OEO inclusion (0 or 1,000 mg kg − 1 ) were evaluated. After 55 days of feeding, sh were exposed to air for 5 min. VC 120 and VC 160 reduced glycemia. In the gills, VC 120 and VC 160 reduced oxidative damage (malondialdehyde, MDA, and carbonylated proteins, PC) and superoxide dismutase (SOD) activity only in the presence of OEO. By contrast, in the absence of VC, OEO increased PC levels and SOD activity. In the liver, all VC concentrations, without OEO, reduced MDA, whereas, in the presence of OEO, vitamin C reduced MDA only at VC 40 e VC 160. All vitamin C concentrations, independently of OEO, reduced PC levels and SOD and catalase (CAT) activity. By contrast, OEO increased CAT and glutathione S-transferase (GST) activity regardless of VC level. Although VC 40 with 1,000 mg kg − 1 OEO was su cient to reduce oxidative damage to lipids in the gills and liver, reduction in protein and lipid damage in the gills was only obtained with at least VC 120 + OEO. Therefore, we recommend simultaneous supplementation of these antioxidants, as their combined use is more e cient in reducing oxidative damage in A. aff. bimaculatus subjected to air.

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Physiological responses of lambariAstyanax altiparanae(Garutti & Britski 2000) to air exposure

Cited by 9 publications

References 16 publications

Clove oil attenuates stress responses in lambari,Astyanax altiparanae

Clove oil attenuates stress responses in lambari,Astyanax altiparanae

Oogenesis is accompanied by cyclic morphological changes in hepatocytes of Neotropical freshwater fish Piabina argentea

Vitamin C combined with oregano essential oil, Origanum vulgare, reduced oxidative damage in Astyanax aff. bimaculatus exposed to air

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