Physiological responses in roots of the grapevine rootstock 140 Ruggeri subjected to Fe deficiency and Fe-heme nutrition

López-Rayo, Sandra; Foggia, Michele Di; Moreira, Erica Rodrigues; Donnini, Silvia; Bombai, Giuseppe; Filippini, Gianfranco; Pisi, Annamaria; Rombolà, Adamo Domenico

doi:10.1016/j.plaphy.2015.07.034

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…After the pioneering works carried out in the ‘80s 5 , 15 , more recent metabolomic investigations support the idea a role that secondary metabolites, and coumarins in particular 16 – 20 , can play a major role in the plants’ response to Fe deficiency. However, there is no literature available on the characterization of root exudates by grapevine, with the sole exception of a paper describing the changes in organic (oxalic, tartaric, malic and ascorbic) acids exuded by Fe-starved 140R and after treatment with Fe-EDDHA and Fe-heme 21 .…”

Section: Introductionmentioning

confidence: 99%

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Marastoni

Lucini

Miras-Moreno

et al. 2020

Sci Rep

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In several cultivation areas, grapevine can suffer from Fe chlorosis due to the calcareous and alkaline nature of soils. This plant species has been described to cope with Fe deficiency by activating Strategy I mechanisms, hence increasing root H+ extrusion and ferric-chelate reductase activity. The degree of tolerance exhibited by the rootstocks has been reported to depend on both reactions, but to date, little emphasis has been given to the role played by root exudate extrusion. We studied the behaviour of two hydroponically-grown, tolerant grapevine rootstocks (Ramsey and 140R) in response to Fe deficiency. Under these experimental conditions, the two varieties displayed differences in their ability to modulate morpho-physiological parameters, root acidification and ferric chelate reductase activity. The metabolic profiling of root exudates revealed common strategies for Fe acquisition, including ones targeted at reducing microbial competition for this micronutrient by limiting the exudation of amino acids and sugars and increasing instead that of Fe(III)-reducing compounds. Other modifications in exudate composition hint that the two rootstocks cope with Fe shortage via specific adjustments of their exudation patterns. Furthermore, the presence of 3-hydroxymugenic acid in these compounds suggests that the responses of grapevine to Fe availability are rather diverse and much more complex than those usually described for Strategy I plants.

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Section: Introductionmentioning

confidence: 99%

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Marastoni

Lucini

Miras-Moreno

et al. 2020

Sci Rep

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“…mendocina strain S5.2 in relation with in silico identification of genomic features associated with grapevine organic acid utilization. Tartaric, malic, oxalic, lactic, citric and succinic acids are among the main organic acids detected in grapevines and root exudates across various genotypes (Andersen and Brodbeck 1989; Cançado et al 2009; Dharmadhikari 1994; Kliewer 1966; Li et al 2013; López-Rayo et al 2015; Mato et al 2007). The illustrated utilization profile of these organic acids by P .…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genomic survey on organic acid utilization profile of Pseudomonas mendocina strain S5.2, a vineyard soil isolate

et al. 2017

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Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5.2. Phenotype microarray analysis has demonstrated that this strain can utilize several organic acids including lactic acid, succinic acid, malic acid, citric acid and fumaric acid as sole growth substrates. Complete genome analysis using single molecule real-time technology revealed that the genome consists of a 5,120,146 bp circular chromosome and a 252,328 bp megaplasmid. A series of genetic determinants associated with the carbon utilization signature of the strain were subsequently identified in the chromosome. Of note, the coexistence of genes encoding several iron–sulfur cluster independent isoenzymes in the genome indicated the importance of these enzymes in the events of iron deficiency. Synteny and comparative analysis have also unraveled the unique features of d-lactate dehydrogenase of strain S5.2 in the study. Collective information of this work has provided insights on the metabolic role of this strain in vineyard soil rhizosphere.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0437-7) contains supplementary material, which is available to authorized users.

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“…Ascorbic acid was purchased from Merck. Ascorbate can be exuded by plants roots [40] and has been used as a model reductant in this study. Ascorbate solutions were prepared freshly directly before application.…”

Section: Methodsmentioning

confidence: 99%

Constraints to Synergistic Fe Mobilization from Calcareous Soil by a Phytosiderophore and a Reductant

Schenkeveld

Kraemer

2018

Soil Syst.

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Synergistic effects between ligand- and reductant-based Fe acquisition strategies can enhance the mobilization of Fe, but also of competing metals from soil. For phytosiderophores, this may alter the time and concentration window of Fe uptake during which plants can benefit from elevated Fe concentrations. We examined how the size of this window is affected by the ligand and reductant concentration and by non-simultaneous addition. To this end, a series of kinetic batch experiments was conducted with a calcareous clay soil to which the phytosiderophore 2′-deoxymugineic acid (DMA) and the reductant ascorbate were added at various concentrations, either simultaneously or with a one- or two-day lag time. Both simultaneous and non-simultaneous addition of the reductant and the phytosiderophore induced synergistic Fe mobilization. Furthermore, initial Fe mobilization rates increased with increasing reductant and phytosiderophore concentrations. However, the duration of the synergistic effect and the window of Fe uptake decreased with increasing reductant concentration due to enhanced competitive mobilization of other metals. Rate laws accurately describing synergistic mobilization of Fe and other metals from soil were parameterized. Synergistic Fe mobilization may be vital for the survival of plants and microorganisms in soils of low Fe availability. However, in order to optimally benefit from these synergistic effects, exudation of ligands and reductants in the rhizosphere need to be carefully matched.

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Physiological responses in roots of the grapevine rootstock 140 Ruggeri subjected to Fe deficiency and Fe-heme nutrition

Cited by 11 publications

References 38 publications

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Phenotypic and genomic survey on organic acid utilization profile of Pseudomonas mendocina strain S5.2, a vineyard soil isolate

Constraints to Synergistic Fe Mobilization from Calcareous Soil by a Phytosiderophore and a Reductant

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