Physiological response to low temperature in the freshwater apple snail, Pomacea canaliculata (Gastropoda: Ampullariidae)

Abstract

SUMMARYCold hardiness of the freshwater apple snail, Pomacea canaliculata, varies seasonally. We investigated lethal factors and physiological changes arising from exposure of P. canaliculata to low temperatures. Snails did not survive freezing. The supercooling point of cold-acclimated (cold tolerant) snails (-6.6±0.8°C) did not differ significantly from that of non-acclimated ones (-7.1±1.5°C) under laboratory conditions. Furthermore, snails died even under more moderately low temperatures approaching 0°C. These results indicate that indirect chilling injury is a factor in the death of P. canaliculata at low temperatures. Regardless of whether the snails were acclimated to low temperatures, all of the dead, and even some of the snails still alive at 0°C, had injured mantles, indicating that the mantle may be the organ most susceptible to the effects of low temperatures. The concentration of glucose in the posterior chamber of the kidney and concentration of glycerol in the digestive gland were significantly higher in cold-acclimated snails than in non-acclimated ones, suggesting carbohydrate metabolic pathways are altered in snails during cold acclimation.

Influence of water temperature on the activity of the freshwater snail Pomacea canaliculata (Caenogastropoda: Ampullariidae) at its southernmost limit (Southern Pampas, Argentina)

“…In coincidence, the increase in weight in P. insularum after 10 days at 35 1C was smaller than that in the range 20-30 1C despite the fact that the ingestion rates showed no differences between these temperatures (Gettys et al, 2008). P. canaliculata from temperate Japan (331N) are able to develop cold hardiness before winter Wada and Matsukura, 2007) but unable to tolerate freezing (Matsukura et al, 2009). Recent molecular studies (Hayes et al, 2008) indicate that Asian P. canaliculata populations are the result of multiple introductions from northern Buenos Aires Province (34-351S) where minimum air temperatures in winter are only 2 1C higher than in our study area (371S).…”

“…The similar latitudes of these Japanese populations and those from the region of origin suggest that cold hardiness could have developed in the native range. P. canaliculata is not usually exposed to subzero temperatures, neither in its southernmost natural populations (our study) nor in the Japanese paddy fields (where snails seek shelter under rice straw; (Matsukura et al, 2009)), which may explain the lack of freezing tolerance reported for Japanese populations.…”

“…Bachmann, 1960;Damborenea, 1996;Martín et al, 2005) and in invaded regions (e.g. Ito, 2002;Wada and Matsukura, 2007;Karatayev et al, 2009), although the physiological processes involved in this behavior are not well understood, except for the acquisition of cold hardiness (Matsukura et al, 2008(Matsukura et al, , 2009). In our field surveys during winter, inactive snails reactivated after a few minutes out of water, even though the air temperature was usually less than 15 1C.…”

Resource-Saving Cleaning Technologies for Power Plant Waste-Water Cooling Ponds

“…It is known that the most sensitive to the action of copper are embryos and larvae of fish [22][23][24][25][26]. In connection with this material, developing embryos, pre-larvae, larvae and carp fry were used in research (Cyprinus carpio, L).…”

Characterizing the effects of temperature on behavioral periodicity in golden apple snails (Pomacea canaliculata)

“…Golden apple snails (Pomacea canaliculata) were obtained from the Gapyeong Golden Apple Snail Farm (http://www.gpwoolunge.co.kr/), and a stock population was maintained in aquariums with dechlorinated tap water (water temperature: 25°C ± 1°C; L16:D8) (Matsukura et al, 2009;Takeichi et al, 2007). The behaviors of test organisms were observed in aquariums (30 cm × 30 cm) filled with water to a depth of 15 cm and with a 3 cm sediment layer at four different temperatures (15°C, 20°C, 25°C, and 30°C; L16:D8).…”