Physiological response dynamics of the brown tide organism Aureococcus anophagefferens treated with modified clay

Zhu, Jianan; Yu, Zhiming; He, Lili; Cao, Xihua; Ji, Hena; Song, Xiuxian

doi:10.1016/j.hal.2019.04.009

Cited by 9 publications

(4 citation statements)

References 52 publications

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“…Notably, uncleared cells (those remaining in the upper layer after the MC treatment) were the object of interest in this paper since they might suffer indirect effects as a result of the MC. Zhu et al found that residual cells after MC treatment showed deformation, irregular depressions and other cell damage [ 44 ]. Evidence from physio-biochemistry and transcriptome analyses has also shown that MC treatment can induce reactive oxygen species (ROS) in residual cells, which leads to oxidative damage and disrupted physiological processes, and inhibited their growth [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Treatment with MC dose-dependently inhibited the growth of solitary cells, which could be attributed to the following two reasons. Firstly, as mentioned above, collision and other interactions between clay particles and cells could cause serious damage in cells [ 29 , 30 , 31 , 44 ]. The collision frequency between the clay particles and P. globosa cells increased with increasing MC concentration, and the percentage of damaged cells in the residual cells also increased [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Modified Clay on Phaeocystis globosa Growth and Colony Formation

Ren

Qiu

et al. 2021

IJERPH

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Phaeocystis globosa is a globally distributed harmful algal blooms (HABs) species dominated by the colonial morphotype, which presents dramatic environmental hazards and poses a threat to human health. Modified clay (MC) can effectively flocculate HAB organisms and prevent their subsequent growth, but the effects of MC on colony-dominated P. globosa blooms remain uncertain. In this paper, a series of removal and incubation experiments were conducted to investigate the growth, colony formation and colony development of P. globosa cells after treatment with MC. The results show that the density of colonies was higher at MC concentrations below 0.2 g/L compared to those in the control, indicating the role of P. globosa colonies in resistance to environmental stress. Concentrations of MC greater than 0.2 g/L could reduce the density of solitary cells and colonies, and the colony diameter and extracellular polysaccharide (EPS) content were also decreased. The adsorption of MC to dissolved inorganic phosphorus (DIP) and the cell damage caused by collision may be the main mechanisms underlying this phenomenon. These results elucidate that the treatment with an appropriate concentration of MC may provide an effective mitigation strategy for P. globosa blooms by preventing their growth and colony formation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Modified Clay on Phaeocystis globosa Growth and Colony Formation

Ren

Qiu

et al. 2021

IJERPH

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“…Neither algae-free cell culture nor ruptured cell suspension caused the rapid death of marine medaka, and this result is consistent with those of previous studies. MC removed microalgae cells and caused the shape of the algae cells to be irregular or the cells to rupture, thus, the algae cells stuck together, and the edges of the cells were blurred [ 48 ]. In this study, MC effectively reduced the cell density of K. mikimotoi cells in the water column and affected the external morphology of the algae cells, causing the algae cells to rupture ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Assessing the Effect of Modified Clay on the Toxicity of Karenia mikimotoi Using Marine Medaka (Oryzias melastigma) as a Model Organism

Zhang

Song

Zhang

et al. 2022

Toxics

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Blooms of the toxic dinoflagellate Karenia mikimotoi could threaten the survival of marine life, and modified clay (MC) is considered a promising method for the control of harmful algal blooms. Here, using marine medaka as the model organism, the toxicity of K. mikimotoi before and after MC disposal was investigated. The results showed that only a certain density of intact K. mikimotoi cells could cause obvious damage to fish gills and lead to rapid death. A systematic analysis of morphology, physiology, and molecular biology parameters revealed that the fish gills exhibited structural damage, oxidative damage, osmotic regulation impairment, immune response activation, and signal transduction enhancement. MC can flocculate K. mikimotoi rapidly in water and reduce its toxicity by reducing the density of intact algae cells and hemolytic toxicity. The results indicate that MC is an effective and safe method for controlling K. mikimotoi blooms.

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“…At the physiological and biochemical levels, Liu et al found that, after treatment with MC, residual cells of Amphidinium carterae and Karenia mikimotoi experienced peroxidation, the antioxidant system was stimulated, and photosynthesis was blocked [11,12]. Ji et al and Zhu et al found that programmed cell death (PCD) occurred in residual cells of Prorocentrum donghaiense and Aureococcus anophagefferens [13,14]. From a molecular perspective, Zhu et al found that transcriptional expression changed in A. anophagefferens residual cells that experienced oxidative stress and disturbances of physiological processes [15].…”

Section: Introductionmentioning

confidence: 99%

Mechanism by Which MC Controls Harmful Algal Blooms Revealed by Cell Morphology of Aureococcus anophagefferens

Zhu

Cao

et al. 2021

IJERPH

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On the basis of field experience, a bloom does not continue after treatment with modified clay (MC), even though the residual harmful algal bloom (HAB) biomass accounts for 20–30% of the initial cells. This interesting phenomenon indicates that, in addition to causing flocculation, MC can inhibit the growth of residual cells. Here, from a cell morphology perspective, Aureococcus anophagefferens was used as a model organism to explore this scientific issue and clarify the mechanism by which MC mitigates harmful algal blooms (HABs). The results showed that, at an ~70% removal efficiency, neutral clay (NC) could not effectively inhibit the growth of residual cells, although it caused various forms of damage to residual cells, such as cell deformation, cell breakage, decreased extracellular polysaccharides (EPS), increased cell membrane permeability, and increased cytoplasmic granularity, due to physical collisions. After modification, some physical and chemical properties of the clay particle surface were changed; for example, the surface electrical properties changed from negative to positive, lamellar spacing increased, hardness decreased, adhesion chains increased, adhesion improved, and the number of absorption sites increased, enhancing the occurrence of chemical and electrochemical effects and physical collisions with residual cells, leading to severe cell deformation and chemical cell breakage. Thus, MC effectively inhibited the growth of residual cells and controlled HABs.

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Physiological response dynamics of the brown tide organism Aureococcus anophagefferens treated with modified clay

Cited by 9 publications

References 52 publications

Effects of Modified Clay on Phaeocystis globosa Growth and Colony Formation

Effects of Modified Clay on Phaeocystis globosa Growth and Colony Formation

Assessing the Effect of Modified Clay on the Toxicity of Karenia mikimotoi Using Marine Medaka (Oryzias melastigma) as a Model Organism

Mechanism by Which MC Controls Harmful Algal Blooms Revealed by Cell Morphology of Aureococcus anophagefferens

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