“…The improved response to 1 day of salt stress in TN6.18 coincides with increased levels of metabolites such as amino, organic acids and other metabolites such as glycerol (Table ) in agreement with previous observations in salt tolerant barley cultivar (Widodo et al ., ), or in Catharanthus roseus under 250 mM NaCl (Chang et al ., ). These metabolites have been considered as osmoprotectors (Sairam & Tyagi, ; Amini & Ehsanpour, ; Abhilash et al ., ; Rybka & Nita, ), and they have also shown to be genotype dependent, taking part of osmotic adjustment to tolerate salinity (Marschner, ; Shabala ; Munns and Gilliham, ; Flowers et al , ). The increased level of osmoregulatory intermediates observed in TN6.18 after 1 day of sudden severe salt treatment (Table ) may be a consequence of a higher TCA cycle flux which would be favoured by the increased rate of mitochondrial electron transport observed in TN6.18 (Fig.…”