Physiological properties and chlorophyll biosynthesis in a Pak-choi (Brassica rapa L. ssp. chinensis) yellow leaf mutant, pylm

Zhang, Kun; Li, Yong; Shan, Xiaofei; Li, Chengyu; Tang, Xiaoyan; Chi, Mingyu; Feng, Hui

doi:10.1007/s11738-016-2321-5

Cited by 39 publications

(49 citation statements)

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“…Wang et al () found that another chlorophyll‐deficient mutant ( cde1 ) was controlled by a dominant gene in B. napus . In B. rapa , the genetic characteristic of the yellow leaf mutant ( pylm ) was controlled by two pairs of recessive genes (Zhang et al ). In addition, a chlorophyll‐deficient mutant ( cdm ) controlled by a cytoplasmic gene was reported in B. campestris (Tang et al ).…”

Section: Introductionmentioning

confidence: 99%

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Huang

et al. 2019

Physiologia Plantarum

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Leaf color mutants are widespread in higher plants and can be used as markers in crop breeding or as important material in understanding the regulatory mechanisms of chlorophyll biosynthesis and chloroplast development. A stably inherited plant etiolated mutation (pem) was obtained from its wild‐type ‘FT’ (a doubled haploid line of the Chinese cabbage variety ‘Fukuda 50’) by combining 60Co‐γ radiation and isolated microspore culture in Chinese cabbage. Compared to the wild‐type ‘FT’, the chlorophyll content in the pem mutant was decreased, the photosynthetic capacity was reduced and the chloroplast development was retarded. These physiological changes may lead to a reduction in growth and yield in the pem mutant line. Genetic analysis showed that the mutant phenotype was controlled by the single recessive nuclear pem gene. The pem gene was mapped to a 25.88 kb region on the A03 chromosome. Cloning and sequencing results showed that there was only one DNA sequence variation in this region, which was a 30 bp deletion on the promoter of Bra024218. Its homologous gene encodes EMBRYO DEFECTIVE 1923 (EMB1923) in Arabidopsis thaliana. We therefore predicted that Bra024218 was the mutated gene associated with etiolated leaves in Chinese cabbage. The pem mutant is a useful line for researching chloroplast development and the mechanism of leaf color mutation in Chinese cabbage.

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Section: Introductionmentioning

confidence: 99%

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Huang

et al. 2019

Physiologia Plantarum

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“…Of the many rice yellow green leaf mutants, ygl1 mutant is due to a missense mutation in a highly conserved residue of YGL1 which encodes the Chl synthase (CHLG) [7], ygl2 mutant is due to an insert mutation of YELLOW-GREEN LEAF2 which encodes Heme Oxygenase 1 [26]. The impaired chloroplast development of pak-choi yellow leaf mutant is associated with blocked Chl biosynthesis process [21]. In Setaria italica , the chlorotic organs is caused by EGY1 (ethylene-dependent gravitropism-deficient and yellow-green 1), which results in premature senescence and damaged PS II function [27].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies revealed that chlorophyllase (Chlase) is involved in Chl degradation in ethylene-treated citrus fruit and could regulate the balance between different plant defense pathways, enhance plant resistance to bacteria [13-15]. Recently, Mutants deficient in Chl biosynthesis and degradation have been identified in many yellow leaf plants, such as rice [16-19], Arabidopsis thaliana [20] and pak-choi [21].…”

Section: Introductionmentioning

confidence: 99%

Physiological and transcriptomic analysis of yellow leaf coloration inPopulus deltoidesMarsh

Zhang

Cui

et al. 2018

Preprint

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As important deciduous tree, Populus deltoides Marsh possesses a high ornamental value 13 for its leaves remaining yellow during the non-dormant period. However, little is known about the 14 regulatory mechanism of leaf coloration in Populus deltoides Marsh. Thus, we analyzed 15 physiological and transcriptional differences of yellow leaves (mutant) and green leaves (wild-type) 16 of Populus deltoides Marsh. Physiological experiments showed that the contents of chlorophyll (Chl) 17 and carotenoid are lower in mutant, the flavonoid content is not differed significantly between 18 mutant and wild-type. Transcriptomic sequencing was further used to identify 153 differentially 19 expressed genes (DEGs). Functional classifications based on Gene Ontology enrichment and 20 Genomes enrichment analysis indicated that the DEGs were involved in Chl biosynthesis and 21 flavonoid biosynthesis pathway. Among these, geranylgeranyl diphosphate (CHLP) genes 22 associated with Chl biosynthesis showed down-regulation, while chlorophyllase (CLH) genes 23 associated with Chl degradation were up-regulated in yellow leaves. The expression levels of these 24 genes were further confirmed using quantitative real-time PCR (RT-qPCR). Furthermore, the 25 measurement of the main precursors of Chl confirmed that CHLP is vital enzymes for the yellow 26 leaf color phenotype. Consequently, the formation of yellow leaf color is due to disruption of Chl 27 synthesis and catabolism rather than flavonoid content. These results contribute to our 28 understanding of mechanisms and regulation of leaf color variation in poplar at the transcriptional 29 level.30

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“…The DH line pylm was obtained by isolated microspore culture of the 'Huaguan' pakchoi variety introduced from Japan musashino seed company. This strain was characterized by yellow leaves and elongated hypocotyls [44]. The parent used for the segregating population development with pylm in this study was 'FT', a DH line derived from Chinese cabbage variety 'Fukuda 50' screened by Shenyang greenstar Chinese cabbage research institute (Shenyang, China), which exhibits folded green leaves [45].…”

Section: Plant Materials and Mapping Population Developmentmentioning

confidence: 99%

“…PCR primers were designed to span the entire putative gene length between the mutant pylm and wild type 'CK-51'. The control line 'CK-51', with a green leaf phenotype, was obtained in the same manner as the pylm mutant during the same period [44]. Candidate genes were cloned following the method referenced from Huang et al [52].…”

Section: Candidate Gene Prediction Andquantitative Real-time Pcr(qrt-mentioning

confidence: 99%

Identification of two recessive etiolation genes (py1, py2) in pakchoi (Brassica rapa L. ssp. chinensis)

Zhang

et al. 2020

Preprint

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Background: Leaf color is a major agronomic trait, which has a strong influence on crop yields. Isolating leaf color mutants can represent valuable materials for research in chlorophyll biosynthesis and metabolism regulation.Results: In this study, we identified a stably inherited yellow leaf mutant derived from ‘Huaguan’ pakchoi variety via isolated microspore culture and designated as pylm. This mutant displayed yellow leaves after germination. Its etiolated phenotype was nonlethal and stable during the whole growth period. Its growth was weak and its hypocotyls were markedly elongated. Genetic analysis revealed that two recessive nuclear genes, named py1 and py2, are responsible for the etiolation phenotype. Bulked segregant RNA sequencing (BSR-Seq) showed that py1 and py2 were mapped on chromosomes A09 and A07, respectively. The genes were single Mendelian factors in F3:4 populations based on a 3:1 phenotypic segregation ratio. The py1 was localized to a 258.3-kb interval on a 34-gene genome. The differentially expressed gene BraA09004189 was detected in the py1 mapping region and regulated heme catabolism. One single-nucleotide polymorphism (SNP) of BraA09004189 occurred in pylm. A candidate gene-specific SNP marker in 1,520 F3:4 yellow-colored individuals co-segregated with py1. For py2, 1,860 recessive homozygous F3:4 individuals were investigated and localized py2 to a 4.4-kb interval. Of the five genes in this region, BraA07001774 was predicted as a candidate for py2. It encoded an embryo defective 1187 and a phosphotransferase related to chlorophyll deficiency and hypocotyl elongation. One SNP of BraA07001774 occurred in pylm. It caused a single amino acid mutation from Asp to Asn. According to quantitative real-time polymerase chain reaction (qRT-PCR), BraA07001774 was downregulated in pylm. Conclusions: Our study identified a Chl deficiency mutant pylm in pakchoi. Two recessive nuclear genes named py1 and py2 had a significant effect on etiolation. Candidate genes regulating etiolation were identified as BraA09004189 and BraA07001774, respectively. These findings will elucidate chlorophyll metabolism and the molecular mechanisms of the gene interactions controlling pakchoi etiolation.

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Physiological properties and chlorophyll biosynthesis in a Pak-choi (Brassica rapa L. ssp. chinensis) yellow leaf mutant, pylm

Cited by 39 publications

References 50 publications

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Mutation in EMB1923 gene promoter is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis)

Physiological and transcriptomic analysis of yellow leaf coloration inPopulus deltoidesMarsh

Identification of two recessive etiolation genes (py1, py2) in pakchoi (Brassica rapa L. ssp. chinensis)

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