Physiological phenotypes and urinary metabolites associated with the psychological changes of healthy human: A study in ‘‘lunar palace 365”

Hao, Zikai; Feng, Siyuan; Zhu, Yinzhen; Yang, Jianlou; Chen, Meng; Hu, Dawei; Liu, Hui; Liu, Hong

doi:10.1016/j.actaastro.2020.04.058

Cited by 9 publications

(5 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, their dietary nutrient levels were calculated rigorously and remained essentially constant. (4) The crew members maintain physical and mental health throughout the experiment [ 13 , 14 ]. (5) The crew members took regular mood measurement tests and feces samples in the same environment to minimize systematic errors.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study, we elucidated the patterns of crew members’ psychological changes in a long-term closed isolation environment with the help of the “Lunar Palace 365” experiment [ 13 , 14 ]. In this study, four potential psychobiotics ( Bacteroides uniformis , Roseburia inulinivorans , Eubacterium rectale , and Faecalibacterium prausnitzii ) were identified through the correlation analysis between gut microbiota and psychological changes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the “Lunar Palace 365” experiment

Hao

Chen

et al. 2023

Microbiome

Self Cite

View full text Add to dashboard Cite

Background Psychological health risk is one of the most severe and complex risks in manned deep-space exploration and long-term closed environments. Recently, with the in-depth research of the microbiota–gut–brain axis, gut microbiota has been considered a new approach to maintain and improve psychological health. However, the correlation between gut microbiota and psychological changes inside long-term closed environments is still poorly understood. Herein, we used the “Lunar Palace 365” mission, a 1-year-long isolation study in the Lunar Palace 1 (a closed manned Bioregenerative Life Support System facility with excellent performance), to investigate the correlation between gut microbiota and psychological changes, in order to find some new potential psychobiotics to maintain and improve the psychological health of crew members. Results We report some altered gut microbiota that were associated with psychological changes in the long-term closed environment. Four potential psychobiotics (Bacteroides uniformis, Roseburia inulinivorans, Eubacterium rectale, and Faecalibacterium prausnitzii) were identified. On the basis of metagenomic, metaproteomic, and metabolomic analyses, the four potential psychobiotics improved mood mainly through three pathways related to nervous system functions: first, by fermenting dietary fibers, they may produce short-chain fatty acids, such as butyric and propionic acids; second, they may regulate amino acid metabolism pathways of aspartic acid, glutamic acid, tryptophan, etc. (e.g., converting glutamic acid to gamma–aminobutyric acid; converting tryptophan to serotonin, kynurenic acid, or tryptamine); and third, they may regulate other pathways, such as taurine and cortisol metabolism. Furthermore, the results of animal experiments confirmed the positive regulatory effect and mechanism of these potential psychobiotics on mood. Conclusions These observations reveal that gut microbiota contributed to a robust effect on the maintenance and improvement of mental health in a long-term closed environment. Our findings represent a key step towards a better understanding the role of the gut microbiome in mammalian mental health during space flight and provide a basis for future efforts to develop microbiota-based countermeasures that mitigate risks to crew mental health during future long-term human space expeditions on the moon or Mars. This study also provides an essential reference for future applications of psychobiotics to neuropsychiatric treatments.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the “Lunar Palace 365” experiment

Hao

Chen

et al. 2023

Microbiome

Self Cite

View full text Add to dashboard Cite

show abstract

“…Future, manned deep-space exploration, including the construction and utilization of artificial lunar bases and the human exploration of Mars [ 1 ], will require the construction of safe, confined habitats, a central component of which is a bioregenerative life support system (BLSS) [ 2 , 3 ]. A BLSS is a small, balanced, self-sufficient artificial ecosystem that encloses air, food, and water loops for crews in confined isolation environments [ 4 , 5 ]. Bio-contamination has long been a subject of great interest in building construction, especially in confined and closed environments such as a BLSS.…”

Section: Introductionmentioning

confidence: 99%

“…Chinese Lunar Palace 1 (LP1) was established as a ground-based BLSS test bed, integrating highly efficient plant cultivation, animal protein production, urine nitrogen recycling, and bioconversion of solid waste [ 5 ]. LP1 was utilized to examine and overcome the various technical and scientific challenges in creating a closed and isolated extraterrestrial living space.…”

Section: Introductionmentioning

confidence: 99%

Surface fungal diversity and several mycotoxin-related genes’ expression profiles during the Lunar Palace 365 experiment

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Chinese Lunar Palace 1 (LP1) is a ground-based bio-regenerative life support system (BLSS) test bed integrating highly efficient plant cultivation, animal protein production, urine nitrogen recycling, and bioconversion of solid waste. To date, there has been no molecular method-based detailed investigation of the fungal community and mycotoxin potential in BLSS habitats. To ensure safe BLSS design for actual space missions, we analyzed the LP1 surface mycobiome and mycotoxin potential during the Lunar Palace 365 project through internal transcribed spacer region 1 (ITS1) amplicon sequencing and quantitative polymerase chain reaction (qPCR) with primers specific for idh, ver1, nor1, tri5, and ITS1. Results The LP1 system exhibited significant differences in fungal community diversity compared to other confined habitats, with higher fungal alpha diversity and different community structures. Significant differences existed in the surface fungal communities of the LP1 habitat due to the presence of different occupant groups. However, there was no significant difference between fungal communities in the plant cabin with various occupants. Source tracker analysis shows that most of the surface fungi in LP1 originated from plants. Regardless of differences in occupants or location, there were no significant differences in mycotoxin gene copy number. Conclusions Our study reveals that plants are the most crucial source of the surface fungal microbiome; however, occupant turnover can induce significant perturbations in the surface fungal community in a BLSS. Growing plants reduced fungal fluctuations, maintaining a healthy balance in the surface fungal microbiome and mycotoxin potential. Moreover, our study provides data important to (i) future risk considerations in crewed space missions with long-term residency, (ii) an optimized design and planning of a space mission that incorporates crew shifts and plant growth, and (iii) the expansion of our knowledge of indoor fungal communities with plant growth, which is essential to maintain safe working and living environments.

show abstract

“…A BLSS is a small, balanced, and self-sufficient man-made ecosystem in which air, food, and water are recycled in a closed and isolated environment [ 7 ]. Chinese Lunar Palace 1 (LP1) is a ground-based BLSS testbed integrating efficient plant cultivation, animal protein production, urinary nitrogen recovery, and solid waste biotransformation [ 8 ]. LP1 can be used to prepare for various technical and scientific challenges in a closed and isolated extraterrestrial living space.…”

Section: Introductionmentioning

confidence: 99%

Microbiomes of air dust collected during the ground-based closed bioregenerative life support experiment "Lunar Palace 365"

Yang

Liu

2022

Environmental Microbiome

Self Cite

View full text Add to dashboard Cite

Background Understanding the dynamics of airborne microbial communities and antibiotic resistance genes (ARGs) in space life support systems is important because potential pathogens and antibiotic resistance pose a health risk to crew that can lead to mission failure. There have been few reports on the distribution patterns of microbiomes and ARGs in space life support systems. In particular, there have been no detailed investigations of microbiomes and/or antibiotic resistance based on molecular methods in long-term confined bioregenerative life support systems (BLSSs). Therefore, in the present study, we collected air dust samples from two crew shifts, different areas, and different time points in the "Lunar Palace 365" experiment. We evaluated microbial diversity, species composition, functional potential, and antibiotic resistance by combining cultivation-independent analyses (amplicon, shot-gun sequencing, and qPCR). Results We found that the bacterial community diversity in the Lunar Palace1 (LP1) system was higher than that in a controlled environment but lower than that in an open environment. Personnel exchange led to significant differences in bacterial community diversity, and source tracking analysis revealed that most bacteria in the air derived from the cabin crew and plants, but no differences in microbial function or antibiotic resistance were observed. Thus, human presence had the strongest effect on the succession of microbial diversity in the BLSSs. Conclusions Our results highlight that microbial diversity in BLSSs is heavily influenced by changes in crew and is unique from other open and controlled environments. Our findings can be used to help develop safe, enclosed BLSS that meet the requirements of human survival and habitation in outer space. In addition, our results can further enhance our understanding of the indoor air microbial community and effectively maintain a safe working and living environment, including plant growth.

show abstract

Physiological phenotypes and urinary metabolites associated with the psychological changes of healthy human: A study in ‘‘lunar palace 365”

Cited by 9 publications

References 73 publications

Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the “Lunar Palace 365” experiment

Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the “Lunar Palace 365” experiment

Surface fungal diversity and several mycotoxin-related genes’ expression profiles during the Lunar Palace 365 experiment

Microbiomes of air dust collected during the ground-based closed bioregenerative life support experiment "Lunar Palace 365"

Contact Info

Product

Resources

About