Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice

Wei, Min; Li, Jia; Yan, Han; Luo, Tao; Huang, Jiehong; Yuan, Yangyang; Hu, Liaoliao; Zheng, Liping

doi:10.3390/ijms23010002

Cited by 8 publications

(6 citation statements)

References 38 publications

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“…A progressive increase in the expression levels of both Suv4-20h1 and Suv4-20h2 has been reported during physiological ovarian aging in mice [45]. However, whether this increase is accompanied by augmented H4K20me3 levels is not known.…”

Section: Agingmentioning

confidence: 99%

The SUV4-20H Histone Methyltransferases in Health and Disease

Gabellini

Pedrotti

2022

IJMS

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The post-translational modification of histone tails is a dynamic process that provides chromatin with high plasticity. Histone modifications occur through the recruitment of nonhistone proteins to chromatin and have the potential to influence fundamental biological processes. Many recent studies have been directed at understanding the role of methylated lysine 20 of histone H4 (H4K20) in physiological and pathological processes. In this review, we will focus on the function and regulation of the histone methyltransferases SUV4-20H1 and SUV4-20H2, which catalyze the di- and tri-methylation of H4K20 at H4K20me2 and H4K20me3, respectively. We will highlight recent studies that have elucidated the functions of these enzymes in various biological processes, including DNA repair, cell cycle regulation, and DNA replication. We will also provide an overview of the pathological conditions associated with H4K20me2/3 misregulation as a result of mutations or the aberrant expression of SUV4-20H1 or SUV4-20H2. Finally, we will critically analyze the data supporting these functions and outline questions for future research.

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Section: Agingmentioning

confidence: 99%

The SUV4-20H Histone Methyltransferases in Health and Disease

Gabellini

Pedrotti

2022

IJMS

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“…To perform hematoxylin-eosin (HE) staining, 5-µm sections were obtained and stained using the HE staining procedure (Baso, China). The maximum section in each tissue was selected for microscopic (Nikon Ci-S, Japan) observation on the pathological structure of tissue and photography, following previously established protocols ( 30 ).…”

Section: Methodsmentioning

confidence: 99%

Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice

Su³

et al. 2023

Front. Immunol.

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IntroductionPremature ovarian failure (POF) is a major cause of infertility among women of reproductive age. Unfortunately, there is no effective treatment available currently. Researchers have shown that immune disorders play a significant role in the development of POF. Moreover, growing evidence suggest that Chitosan Oligosaccharides (COS), which act as critical immunomodulators, may have a key role in preventing and treating a range of immune related reproductive diseases.MethodsKM mice (6-8 weeks) received a single intraperitoneal injection of cyclophosphamide (CY, 120mg/kg) and busulfan (BUS, 30mg/kg) to establish POF model. After completing the COS pre-treatment or post-treatment procedures, peritoneal resident macrophages (PRMs) were collected for neutral erythrophagocytosis assay to detect phagocytic activity. The thymus, spleen and ovary tissues were collected and weighed to calculate the organ indexes. Hematoxylin-eosin (HE) staining was performed to observe the histopathologic structure of those organs. The serum levels of estrogen (E2) and progesterone (P) were measured via the enzyme-linked immunosorbent assay (ELISA). The expression levels of immune factors including interleukin 2 (IL-2), interleukin 4 (IL-4), and tumor necrosis factor α (TNF-α), as well as germ cell markers Mouse Vasa Homologue (MVH) and Fragilis in ovarian tissue, were analyzed by Western blotting and qRT-PCR. In addition, ovarian cell senescence via p53/p21/p16 signaling was also detected.ResultsThe phagocytic function of PRMs and the structural integrity of thymus and spleen were preserved by COS treatment. The levels of certain immune factors in the ovaries of CY/BUS- induced POF mice were found to be altered, manifested as IL-2 and TNF-α experiencing a significant decline, and IL-4 presenting a notable increase. Both pre-treatment and post-treatment with COS were shown to be protective effects against the damage to ovarian structure caused by CY/BUS. Senescence-associated β-galactosidase (SA-β-Gal) staining results showed that COS prevents CY/BUS-induced ovarian cell senescence. Additionally, COS regulated estrogen and progesterone levels, enhanced follicular development, and blocked ovarian cellular p53/p21/p16 signaling which participating in cell senescence.ConclusionCOS is a potent preventative and therapeutic medicine for premature ovarian failure by enhancing both the ovarian local and systemic immune response as well as inhibiting germ cell senescence.

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“…Furthermore, our previous study revealed that the global Ksuc in mouse ovary increases signi cantly with advancing age [12]. Thus, in this study we further evaluated the effect of excessive Ksuc on ovarian function to reveal the relationship between Ksuc and ovarian aging.…”

Section: Introductionmentioning

confidence: 92%

The emerging role of lysine succinylation in ovarian aging

Zhang

et al. 2022

Preprint

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Background Ovarian aging is a process of decline in its reserve leading to ovary dysfunction and even reduced health quality in offspring. However, aging-related molecular pathways in the ovary remain obscure. Lysine succinylation (Ksuc), a newly post-translational modification (PTM), has been found to be broadly conserved in both eukaryotic and prokaryotic cells, and associated with multiple pathophysiological processes. There are no relevant reports revealing a link between the molecular mechanisms of ovarian aging and Ksuc. Methods The level of Ksuc in ovaries of aged and premature ovarian insufficiency (POI) mice and granulosa cells (GCs) derived from follicular fluid of women with POI were detected by immunoblotting and immunohistochemical. To further explore the role of Ksuc in ovarian aging, using in vitro mouse ovary tissue culture and an in vivo mouse model with changed Ksuc level. Results Increased Ksuc in ovaries of aged and POI mice and GCs derived from follicular fluid of women with POI, and distribution of Ksuc in various types of mice ovarian cells and the high level of Ksuc in GCs were revealed. Histological assessments and hormone levels analyses showed that the high Ksuc level down-regulated the ovarian index and the anti-Müllerian hormone (AMH) and estrogen levels, and increased follicular atresia. Moreover, in the high Ksuc groups, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) intensities and the expression of Cleaved-caspase-3 increased and the expression of B-cell lymphoma-2 (Bcl-2) decreased together with positively-expressed P21, an aging-related marker. These results suggest that ovarian aging is likely associated with alteration in Ksuc. Conclusion The present study has identified Ksuc in mouse ovary and human GCs and found that high Ksuc level most likely contributes to ovarian aging which is expected further investigation to provide new information for delaying physiological ovarian aging and treating pathological ovarian aging.

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Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice

Cited by 8 publications

References 38 publications

The SUV4-20H Histone Methyltransferases in Health and Disease

The SUV4-20H Histone Methyltransferases in Health and Disease

Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice

The emerging role of lysine succinylation in ovarian aging

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