Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants

Nicolas-Santiago, Soledad De; Regalado‐González, Carlos; García-Almendárez, Blanca E.; Fernandez, F; Téllez‐Jurado, Alejandro; Huerta‐Ochoa, Sergio

doi:10.2225/vol9-issue1-fulltext-2

Cited by 34 publications

(24 citation statements)

References 16 publications

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“…Similar results were found by Liu et al (2014). de Nicolas-Santiago et al (2006) reported that differential mannanase production in original A. niger and mutants was associated with different conidial morphologies and diameters. These results proved that the physiological properties of fungal spores were related to their physiological metabolic functions.…”

Section: Discussionsupporting

confidence: 88%

Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

Chen

Wang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus niger, and to analyze the relationship between changes in the physiological properties of A. niger induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain.

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Section: Discussionsupporting

confidence: 88%

Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

Chen

Wang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…The concentration of 0.15% (w/v) SDS is 10 times higher than that used for the same purposes in A. oryzae (Guilarte et al, 2009) which demonstrates the higher resistance of the PR-142 strain. The lethal dose of UV reached 50% at 1.5 min, which is less than the 3 min obtained by other A. niger strains as reported by De Nicolás-Santiago et al (2006) for the production of mutants with high production capacity of xylans and mannans. This reduction could be expected by considering the higher dose of UV rays used in the current work.…”

Section: Discussioncontrasting

confidence: 52%

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Castillo¹,

DÃez-Municio²,

Cruz³

et al. 2017

Afr. J. Biotechnol.

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A strain of Aspergillus niger PR-142 native to northern coast of Peru was subjected to successive processes of mutagenesis by ultraviolet light (UV) irradiation at 253.7 nm to increase the production of fructooligosaccharides (FOS). An initial selection was made by considering the mutants with increased invertase activity followed by the measurement of β-fructosyltransferase (FTase) activity both in mycelium and extracellular environment. Five selected mutants, which showed increased values of mycelium invertase activity (ranging from 101 to 128% as compared to the parent strain) at 40°C and sodium dodecylsulfate 0.15 (w/v), were grown in a fermentative medium in 50 mL conical tubes on a rotary shaker, and their FTase activity was determined. The 6-M69 mutant showed the most active mycelium activity of 1.5 fold as compared to the parent strain. When the same reaction was performed between 1 to 4 h, at the 3 rd h, the mycelium FTase activity significantly increased up to 7 and 3 times in the mutant and parental strain, respectively. Finally, 4 mutants and the parental PR-142 were genetically characterized using inter simple sequence repeat polymerase chain reaction (ISSR-PCR) molecular markers. This analysis showed a significant 33% polymorphic bands between the parent and mutant markers, and 20 bands were unique to the mutants.

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“…This wild type culture was randomly mutated using ultraviolet light exposure according to the modified method of Nicolás-Santiago et al (2006) by Jacobs University gGmbH, Bremen and used as the mutant strain in this study.…”

Section: Microorganism and Propagationmentioning

confidence: 99%

Valorization of wheat bran for the production of polygalacturonase in SSF of Aspergillus sojae

Demi̇r

Tarı

2014

Industrial Crops and Products

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a b s t r a c tWheat bran, among various agro industrial by products, screened for the production of polygalacturonase (PG) in solid-state fermentation of Aspergillus sojae mutant strain, was found to be the most suitable substrate without the addition of any nutritive or inducing supplement. It was further characterized for its physicochemical composition and particle size distribution. The process conditions that favored the PG production using this substrate were determined as; 10 7 spore/g substrate inoculum concentration, 4 days of fermentation, 37• C of incubation temperature, 62% initial moisture content, water as the moistening agent, 100-250 m particle size of wheat bran, 3 times/day agitation and spore solution as the inoculum type which resulted into maximum PG activity of 535.4 U/g substrate. Overall, this optimization process resulted in 7.3 and 3.9 fold of significant enhancement in the PG activity and productivity, respectively.

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Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants

Cited by 34 publications

References 16 publications

Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Valorization of wheat bran for the production of polygalacturonase in SSF of Aspergillus sojae

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