Physiological mechanism of transglutaminase-mediated improvement in salt tolerance of cucumber seedlings

Shu, Sheng; Tang, Yuanyuan; Zhou, Xinpeng; Jahan, Mohammad Shan; Sun, Jian; Wang, Yu; Guo, Shirong

doi:10.1016/j.plaphy.2020.09.010

Cited by 8 publications

(7 citation statements)

References 48 publications

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“…Salt stress can induce endogenous PAs in plants [ 26 , 27 , 28 ]. In order to explore whether CsCDPK6 is involved in the regulation of endogenous PA production under salt stress, the content of PA changes was further analyzed.…”

Section: Resultsmentioning

confidence: 99%

CsCDPK6, a CsSAMS1-Interacting Protein, Affects Polyamine/Ethylene Biosynthesis in Cucumber and Enhances Salt Tolerance by Overexpression in Tobacco

Zhu

Jahan

et al. 2021

IJMS

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S-adenosylmethionine synthetase (SAMS) plays a crucial role in regulating stress responses. In a recent study, we found that overexpression of the cucumber gene CsSAMS1 in tobacco can affect the production of polyamines and ethylene, as well as enhancing the salt stress tolerance of tobacco, but the exact underlying mechanisms are elusive. The calcium-dependent protein kinase (CDPK) family is ubiquitous in plants and performs different biological functions in plant development and response to abiotic stress. We used a yeast two-hybrid system to detect whether the protein CDPK6 could interact with SAMS1 and verified their interaction by bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP) assays. To further explore the function of cucumber CDPK6, we isolated and characterized CsCDPK6 in cucumber. CsCDPK6 is a membrane protein that is highly expressed under various abiotic stresses, including salt stress. It was also observed that ectopic overexpression of CsCDPK6 in tobacco enhanced salt tolerance. Under salt stress, CsCDPK6-overexpressing lines enhanced the survival rate and reduced stomatal apertures in comparison to wild-type (WT) lines, as well as lowering malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents and causing less relative electrolyte leakage. Moreover, repression of CsCDPK6 expression by virus-induced gene silencing (VIGS) in cucumber seedling cotyledons under salt stress increased ethylene production and promoted the transformation from putrescine (Put) to spermidine (Spd) and spermine (Spm). These findings shed light on the interaction of CsSAMS1 and CsCDPK6, which functions positively to regulate salt stress in plants.

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Section: Resultsmentioning

confidence: 99%

CsCDPK6, a CsSAMS1-Interacting Protein, Affects Polyamine/Ethylene Biosynthesis in Cucumber and Enhances Salt Tolerance by Overexpression in Tobacco

Zhu

Jahan

et al. 2021

IJMS

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“…Decreases in photosynthetic capacity are usually mainly due to stomatal and/or nonstomatal limitations. The observation of key parameters with chlorophyll fluorescence imaging can directly reflect the photochemical efficiency of plants, which is the key to our understanding of photosynthetic physiology [ 27 , 28 , 31 ]. In this study, as the treatment time increased, the gas exchange parameters such as the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of cucumber seedling leaves under low light stress were all significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

“…Sucrose synthase (SS) and sucrose phosphate synthase (SPS) are the key enzymes in the sucrose synthesis pathway. In leaves, SPS plays a role as the limiting factor in the export of photoassimilates to sink tissues [ 29 , 31 , 46 – 49 ]. In this study, SPS and SS gene expression and sucrose synthase and sucrose phosphate synthase enzyme activity were inhibited under low light stress, so the sucrose and soluble sugar contents in cucumber leaves were significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

“…To maintain the redox balance, plants usually quench ROS with the help of antioxidant enzymes and nonenzymatic systems. Antioxidant enzymes and other antioxidants are important components to eliminate ROS under abiotic stress [31,33]. Some recent studies have found that several stresses can stimulate the RBOH gene to induce the production of ROS, which is essential for rapid defence signalling in the early stage of stress [30,33]..…”

Section: Discussionmentioning

confidence: 99%

“…According to the methods of Zhang et al [30] and Shu et al [31]. For the estimation of H 2 O 2 , a 0.3 g sample was homogenized in 3 mL of 0.1% trichloroacetic acid (TCA) (w/v) followed by centrifugation at 12000×g for 15 min at 4 °C.…”

Section: H 2 O 2 and Mda Contentsmentioning

confidence: 99%

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Physiological mechanism of strigolactone enhancing tolerance to low light stress in cucumber seedlings

et al. 2022

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Strigolactone is a newly discovered type of plant hormone that has multiple roles in modulating plant responses to abiotic stress. Herein, we aimed to investigate the effects of exogenous GR24 (a synthetic analogue of strigolactone) on plant growth, photosynthetic characteristics, carbohydrate levels, endogenous strigolactone content and antioxidant metabolism in cucumber seedlings under low light stress. The results showed that the application of 10 μM GR24 can increase the photosynthetic efficiency and plant biomass of low light-stressed cucumber seedlings. GR24 increased the accumulation of carbohydrates and the synthesis of sucrose-related enzyme activities, enhanced antioxidant enzyme activities and antioxidant substance contents, and reduced the levels of H2O2 and MDA in cucumber seedlings under low light stress. These results indicate that exogenous GR24 might alleviate low light stress-induced growth inhibition by regulating the assimilation of carbon and antioxidants and endogenous strigolactone contents, thereby enhancing the tolerance of cucumber seedlings to low light stress.

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Polyamines metabolism and their biological role in plant cells: what do we really know?

Kolesnikov,

Kretynin,

Filepova

et al. 2024

Phytochem Rev

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Physiological mechanism of transglutaminase-mediated improvement in salt tolerance of cucumber seedlings

Cited by 8 publications

References 48 publications

CsCDPK6, a CsSAMS1-Interacting Protein, Affects Polyamine/Ethylene Biosynthesis in Cucumber and Enhances Salt Tolerance by Overexpression in Tobacco

CsCDPK6, a CsSAMS1-Interacting Protein, Affects Polyamine/Ethylene Biosynthesis in Cucumber and Enhances Salt Tolerance by Overexpression in Tobacco

Physiological mechanism of strigolactone enhancing tolerance to low light stress in cucumber seedlings

Polyamines metabolism and their biological role in plant cells: what do we really know?

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