Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex® Multiplex technology

Thrailkill, Kathryn M.; Moreau, Cindy S.; Cockrell, Gael; Simpson, Pippa; Goel, Rajiv; North, Paula E.; Fowlkes, John L.; Bunn, R. Clay

doi:10.1515/cclm.2005.238

Cited by 39 publications

(35 citation statements)

References 38 publications

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“…This positive correlation had been asserted in some previous studies, too (Thrailkill, 2005;KomosinskaVassev et al, 2011). It is proposed that increase in MMP-3 serum level with increasing age is the result of hormonal change and physiological process (Thrailkill, 2005). In this study serum MMP-3 concentration was higher in females compared to males.…”

Section: Resultssupporting

confidence: 65%

Serum Level of MMP-3 in Patients with Oral Squamous Cell Carcinoma - Lack of Association with Clinico-pathological Features

Andisheh-Tadbir

Purshahidi²,

Ebrahimi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Resultssupporting

confidence: 65%

Serum Level of MMP-3 in Patients with Oral Squamous Cell Carcinoma - Lack of Association with Clinico-pathological Features

Andisheh-Tadbir

Purshahidi²,

Ebrahimi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…TNF-α was assessed using Fluorokine ® MultiAnalyte Profiling (F-MAP) kits from R&D Systems (Minneapolis, MN, USA) as previously described. 27 Kits were run on a BioPlex® dual laser Bioanalyzer (Luminex Corp., Austin, TX, USA) according to the manufacturer's instructions. The minimal detectable dose of TNF-α was less than 3 pg/ml.…”

Section: Measurementsmentioning

confidence: 99%

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Guillou

Tamion

Jouet

et al. 2012

Diabetes and Vascular Disease Research

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Background: Diabetes is a risk factor for perioperative complications after cardiac surgery. We studied its effects on mesenteric endothelial function in a cardiopulmonary bypass (CPB) model. Methods: Forty Wistar rats were divided into four groups: sham (D-CPB-), cardiopulmonary bypass (D-CPB+), diabetic (D+CPB-) and diabetic that have undergone CPB (D+CPB+). Two samples of mesenteric artery were used for nitric oxide synthase (NOS) Western blot analysis, and two others for assessing contractile response and endothelium relaxations. Nitrite products and tumour necrosis factor-alpha (TNF-α) were assessed as markers of inflammatory response. Results: We observed an enhanced contractile response to the α-adrenergic agonist associated with impairment of mesenteric vasorelaxation in D+CPB+ rats. Western immunoblot analysis of D+CPB+ highlighted an additive effect of hyper-expression of inducible NOS. A significantly increased inflammatory response was observed after CPB in diabetic animals. Conclusions: This work confirms the potential deleterious impact of diabetes on the mesenteric endothelium during CPB in cardiac surgery.

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“…Specimens were analyzed in duplicate on a Luminex 100 Bioanalyzer (Luminex, Austin, TX) as previously described (minimal detection limit, 25.4 pg/ml) (17,18).…”

Section: Research Design Andmentioning

confidence: 99%

Matrix Metalloproteinase-2 Dysregulation in Type 1 Diabetes

et al. 2007

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OBJECTIVE -Dysregulation of matrix metalloproteinase (MMP)-2 may contribute pathologically to the development of diabetes complications, including diabetic retinopathy and coronary and peripheral arterial disease. Our objective was to explore whether systemic MMP-2 dysregulation could be demonstrated in type 1 diabetes and to determine how MMP-2 concentration relates to disease status.RESEARCH DESIGN AND METHODS -In this cross-sectional study, MMP-2 concentrations and MMP-2 activity were measured in plasma and timed urine samples from 93 type 1 diabetic and 50 healthy control subjects, aged 14 -40 years. Relationships between MMP-2 concentrations in these biological fluids and subject characteristics (sex, age, and duration of type 1 diabetes), indexes of glycemic control (A1C, fasting plasma glucose, and continuous glucose monitoring system average daily glucose), and measurements of renal function (urinary albumin excretion and glomerular filtration rate) were examined.RESULTS -Urine and plasma MMP-2 concentrations and plasma MMP-2 activity were all significantly elevated in type 1 diabetic subjects compared with those in control subjects. Urine MMP-2 concentrations, in particular, were correlated with several clinical parameters that infer increased risk for diabetic comorbidity and specifically for diabetic nephropathy, including higher A1C, longer duration of disease, evidence of renal hyperfiltration, and the presence of microalbuminuria.CONCLUSIONS -Urine and plasma MMP-2 concentrations are dysregulated in type 1 diabetes; urinary excretion of MMP-2, in particular, might provide a unique biomarker of diabetes-induced intrarenal pathologic processes. Diabetes Care 30:2321-2326, 2007M atrix metalloproteinases (MMPs) constitute a group of enzymes that hydrolyze protein components of the extracellular matrix (1). The subgroup of MMPs known as gelatinases, specifically gelatinase A (MMP-2) and gelatinase B (MMP-9) digest collagen, denatured collagens (i.e., gelatins), laminin, elastin, and fibronectin, among other substrates (2), and have been implicated in the pathological processes that contribute to fibrotic diseases, tumor progression, and inflammation (1,3,4).Dysregulation of gelatinase activity has also been implicated in the pathophysiology of diabetes complications. Specifically, gelatinase concentrations are increased in the systemic circulation ) and in the vitreous (MMP-2 [6] and MMP-9 [7]) of type 1 diabetic patients with diabetic retinopathy. Elevated retinal levels of MMP-2 and MMP-9 have also been demonstrated in an animal model of diabetic retinopathy (8). Increased circulating concentrations of MMP-2 have been observed in pediatric patients with type 1 diabetes who developed microangiopathy over a 5-year interval (9). Systemic concentrations of MMP-2 and MMP-9, in addition to gelatinase activity levels, are also increased in patients with type 2 diabetes and peripheral arterial disease (10).Data suggesting a link between MMP-2 dysregulation and diabetic nephropathy also exist but appear contra...

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Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex® Multiplex technology

Cited by 39 publications

References 38 publications

Serum Level of MMP-3 in Patients with Oral Squamous Cell Carcinoma - Lack of Association with Clinico-pathological Features

Serum Level of MMP-3 in Patients with Oral Squamous Cell Carcinoma - Lack of Association with Clinico-pathological Features

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Matrix Metalloproteinase-2 Dysregulation in Type 1 Diabetes

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