Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Cao, Chuanwang; Sun, Lili; Du, Hui; Moural, Timothy W.; Bai, Hua; Liu, Peng; Zhu, Fang

doi:10.1016/j.pestbp.2019.07.002

Cited by 27 publications

(26 citation statements)

References 67 publications

(85 reference statements)

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“…Overexpression of the GPCR gene from the Culex mosquito in a Drosophila strain using transgenic technology showed increased resistance to insecticide and induced overexpression of P450s in the Drosophila [ 50 ]. A similar study in the gypsy moth Lymantria dispar that employed both RNAi and transgenic Drosophila techniques identified a methuselah-like GPCR that was functionally involved in the moth’s deltamethrin resistance and the regulation of its metabolic enzyme coding gene expression [ 145 ].…”

Section: Gene Expression and Functional Characterization Of The Gpcrs In Insectsmentioning

confidence: 99%

“…Four of Culex GPCR genes were identified as upregulated in the resistant strains, including the GPCR gene identified previously [ 141 ]. Consequently, several GPCR genes have been reported to be overexpressed in resistant insects, including mosquito Culex pipiens pallens [ 172 ], Asian gypsy moth, L. dispar [ 145 ], and house flies, M. domestica [ 140 ]. All these discoveries reveal the importance of the GPCRs involved in insecticide resistance of insects [ 49 ].…”

Section: Gpcrs the Intracellular Pathway And The Function Into Insecticide Resistancementioning

confidence: 99%

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G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

Liu

Wang

et al. 2021

IJMS

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G-protein-coupled receptors (GPCRs) are known to play central roles in the physiology of many organisms. Members of this seven α-helical transmembrane protein family transduce the extracellular signals and regulate intracellular second messengers through coupling to heterotrimeric G-proteins, adenylate cyclase, cAMPs, and protein kinases. As a result of the critical function of GPCRs in cell physiology and biochemistry, they not only play important roles in cell biology and the medicines used to treat a wide range of human diseases but also in insects’ physiological functions. Recent studies have revealed the expression and function of GPCRs in insecticide resistance, improving our understanding of the molecular complexes governing the development of insecticide resistance. This article focuses on the review of G-protein coupled receptor (GPCR) signaling pathways in insect physiology, including insects’ reproduction, growth and development, stress responses, feeding, behaviors, and other physiological processes. Hormones and polypeptides that are involved in insect GPCR regulatory pathways are reviewed. The review also gives a brief introduction of GPCR pathways in organisms in general. At the end of the review, it provides the recent studies on the function of GPCRs in the development of insecticide resistance, focusing in particular on our current knowledge of the expression and function of GPCRs and their downstream regulation pathways and their roles in insecticide resistance and the regulation of resistance P450 gene expression. The latest insights into the exciting technological advances and new techniques for gene expression and functional characterization of the GPCRs in insects are provided.

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Section: Gene Expression and Functional Characterization Of The Gpcrs In Insectsmentioning

confidence: 99%

Section: Gpcrs the Intracellular Pathway And The Function Into Insecticide Resistancementioning

confidence: 99%

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

Liu

Wang

et al. 2021

IJMS

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“…Rhodopsin receptors perform vital functions in both the insect's reproduction system [22] and its vision [107]. Class B GPCRs such as methuselah receptors are also involved in insect longevity [135], and oxidative stress resistance [136] and diuretic hormone receptors regulate the body temperature and homeostasis [114]; GPCRs in the Class C family, such as metabotropic GABA receptors, are important for the central nervous system (CNS) [118].…”

Section: Receptors Of Gpcr Involved In Insect Physiology or Insecticide Resistance That Are Potential Targets For Insecticide Developmentmentioning

confidence: 99%

“…In addition to the vital functions they perform in the insect’s physiology, GPCRs are also involved in insecticide resistance. The methuselah-like receptor in L. dispar [ 135 ], the calcitonin receptor and opsin receptor in Cx. quinquefasciatus [ 42 ] and the arrestin gene in Culex pipiens [ 137 ] have all been shown to be involved in the upregulation of detoxified enzymes such as cytochrome P450.…”

Section: Whole Genome Sequencing and Transcriptome Analysis—sequence Comparison And Gpcr Characterization In Insectsmentioning

confidence: 99%

G-Protein Coupled Receptors (GPCRs) in Insects—A Potential Target for New Insecticide Development

Liu

Wang

et al. 2021

Molecules

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G-protein coupled receptors (GPCRs) play important roles in cell biology and insects’ physiological processes, toxicological response and the development of insecticide resistance. New information on genome sequences, proteomic and transcriptome analysis and expression patterns of GPCRs in organs such as the central nervous system in different organisms has shown the importance of these signaling regulatory GPCRs and their impact on vital cell functions. Our growing understanding of the role played by GPCRs at the cellular, genome, transcriptome and tissue levels is now being utilized to develop new targets that will sidestep many of the problems currently hindering human disease control and insect pest management. This article reviews recent work on the expression and function of GPCRs in insects, focusing on the molecular complexes governing the insect physiology and development of insecticide resistance and examining the genome information for GPCRs in two medically important insects, mosquitoes and house flies, and their orthologs in the model insect species Drosophila melanogaster. The tissue specific distribution and expression of the insect GPCRs is discussed, along with fresh insights into practical aspects of insect physiology and toxicology that could be fundamental for efforts to develop new, more effective, strategies for pest control and resistance management.

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“…An exploration of GPCRs in Tribolium castaneum indicated mth is also important for larval molt and metamorphosis (Bai, Zhu, Shah, & Palli, 2011). Moreover, mth gene mutation led D. melanogaster to become tolerant to dichlorvos (Pandey et al, 2015), but insect response to insecticide exposure was also altered with changes in the levels of mth expression (Cao et al, 2019; Li et al, 2015; Lucas et al, 2019; Ma, Zhang, You, Zeng, & Gao, 2020). Similarly to the early discussed synergism of ABCCs and phase II conjugation enzymes in insect response to insecticide exposure, mth is co-expressed with the phase I detoxification P450 enzymes, contributing to insect resistance to insecticides (Cao et al, 2019; Li et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

There is more than chitin synthase in insect resistance to benzoylureas: Molecular markers associated with teflubenzuron resistance inSpodoptera frugiperda

Nascimento

Pavinato

Rodrigues

et al. 2020

Preprint

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Chitin synthesis inhibitors are successfully used in pest control and are an excellent option for use in integrated pest management programs due to their low non-target effects. Reports on field-evolved resistance of lepidopteran pests to chitin synthesis inhibitors and the selection of laboratory resistant strains to these products require a detailed investigation on the resistance mechanisms and on the identification of molecular markers to support the implementation of efficient monitoring and resistance management programs. Teflubenzuron is a chitin synthesis inhibitor highly effective in controlling lepidopteran pests, including nowadays the world widely distributed fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae). We report the selection of a laboratory strain of S. frugiperda resistant to teflubenzuron, and its use for the characterization of the inheritance of resistance, evaluation of cross-resistance to other chitin-synthesis inhibitors and the identification of a set of single nucleotide polymorphisms (SNPs) for use as candidate molecular markers for monitoring the evolution of resistance of S. frugiperda to teflubenzuron. The resistance of the selected strain of S. frugiperda to teflubenzuron was characterized as polygenic, autosomal, and incompletely recessive. The resistance ratio observed was nearly 1,365-fold. Teflubenzuron-resistant strain showed some cross-resistance to lufenuron and novaluron but not to chlorfluazuron. We also detected a set of 72 SNPs that could support monitoring of the resistance frequency to teflubenzuron in field populations. Our data contribute to the understanding of the resistance mechanisms and the inheritance of polygenic resistance of S. frugiperda to benzoylureas. We also contribute with candidate markers as tools for monitoring the emergence and spread of teflubenzuron resistance in S. frugiperda.

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Physiological functions of a methuselah-like G protein coupled receptor in Lymantria dispar Linnaeus

Cited by 27 publications

References 67 publications

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology

G-Protein Coupled Receptors (GPCRs) in Insects—A Potential Target for New Insecticide Development

There is more than chitin synthase in insect resistance to benzoylureas: Molecular markers associated with teflubenzuron resistance inSpodoptera frugiperda

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