Physiological effects of nickel chloride on the freshwater cyanobacterium &amp;lt;i&amp;gt;Synechococcus&amp;lt;/i&amp;gt; sp. IU 625

Nohomovich, Brian; Nguyen, Bao; Quintanilla, Michael; Lee, Lee H.; Murray, Sean R.; Chu, Tin-Chun

doi:10.4236/abb.2013.47a2002

Cited by 9 publications

(10 citation statements)

References 18 publications

(14 reference statements)

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Section: Discussionmentioning

confidence: 99%

“…Our results showed that Synechococcus species were significantly enriched in the bacterial communities downstream of the complete hydropower stations. Given that Synechococcus species are reported that cause freshwater cyanobacteria algal blooms ( Glibert et al, 2009 ; Wall et al, 2012 ; Nohomovich et al, 2013 ; Paerl et al, 2016 ), the increase in the proportion of Synechococcus increases the risk of freshwater algal blooms. It should be emphasized that although the sampling sites S13 and S15 were downstream of hydropower stations, they were very close to the stations (about 1 km), and given the water flow, the sampling sites likely represent the situation upstream.…”

Section: Discussionmentioning

confidence: 99%

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Influence of hydropower stations on the water microbiota in the downstream of Jinsha River, China

Chen

Zhou

Cao³

et al. 2020

PeerJ

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Construction of hydropower stations has been an important approach to meet China’s increasing power demand, but the impact of construction of hydropower stations on river microbiota is not fully understood. To evaluate this, the microbial composition from 18 sampling sites in the downstream of Jinsha River of China, upstream and downstream of two completed and two under-construction hydropower stations, were analyzed using high-throughput 16S rRNA gene sequencing. Three independent samples from each site were analyzed. A total of 18,683 OTUs from 1,350 genera were identified at 97% sequence similarity. Our results showed that the completion of hydropower stations would significantly increase the relative abundances of Acidobacteria, Chlorobi, Chloroflexi, Cyanobacteria, Nitrospirae, and Planctomycetes, especially the relative abundance of Synechococcus dOTUs and thus increase the risk of algal blooms. PCA based on all KEGG pathways and the significantly different KEGG pathways showed the predicted metabolic characteristics of the water microbiota by PICRUSt in the activated hydropower station group were significant difference to the other groups. Results from canonical correspondence analysis showed that water temperature and dissolved oxygen had significant effects on microbiota composition. These results are important for assessing the impact of hydropower stations on river microbiota and their potential environmental risks.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of hydropower stations on the water microbiota in the downstream of Jinsha River, China

Chen

Zhou

Cao³

et al. 2020

PeerJ

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“…The presence of 10 mg/L Ni reduced growth of the cyanobacterium Synechococcus sp. and was accompanied by cell morphological changes and elevated Ni content [145]. Cd at a concentration of 17 mg/L inhibited the growth by 51% and photosynthetic oxygen evolution by 30% in Scenedesmus armatus culture cultivated in the presence of 0.1% CO 2 , but the increase in CO 2 to 2% improved protection of Scenedesmus cells against cadmium, as only 8% inhibition of oxygen evolution and 27% growth inhibition was observed [146].…”

Section: Heavy Metal Ionsmentioning

confidence: 97%

Effect of Lignocellulose Related Compounds on Microalgae Growth and Product Biosynthesis: A Review

et al. 2014

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Microalgae contain valuable compounds that can be harnessed for industrial applications. Lignocellulose biomass is a plant material containing in abundance organic substances such as carbohydrates, phenolics, organic acids and other secondary compounds. As growth of microalgae on organic substances was confirmed during heterotrophic and mixotrophic cultivation, lignocellulose derived compounds can become a feedstock to cultivate microalgae and produce target compounds. In this review, different treatment methods to hydrolyse lignocellulose into organic substrates are presented first. Secondly, the effect of lignocellulosic hydrolysates, organic substances typically present in lignocellulosic hydrolysates, as well as minor co-products, on growth and accumulation of target compounds in microalgae cultures is described. Finally, the possibilities of using lignocellulose hydrolysates as a common feedstock for microalgae cultures are evaluated.Keywords: lignocellulose; hydrolysis; microalgae; cultivation; extraction; bioproducts OPEN ACCESSEnergies 2014, 7 4447 Microalgae: A Source of Valuable CompoundsMicroalgae include several groups of microorganisms that belong to the Prokaryota or Eukaryota, typically found in fresh water or marine systems in single cell forms or in groups. They are capable of performing photosynthesis, producing approximately half of the atmospheric oxygen while using the greenhouse gas carbon dioxide to grow photoautotrophically [1]. Microalgae contain valuable compounds such as lipids, proteins and pigments (Table 1) which have substantial potential for commercial applications. Microalgae cells accumulate lipids which include triacylglycerides (TAGs), polyunsaturated fatty acids (PUFAs) and sterols [2]. These lipids constitute storage materials or membrane structural components in microalgae and can be used for biofuel, food supplement and pharmaceutical production. Indeed, fossil fuels are nowadays still the main source of carbon based fuels, the exploitation of which causes emission of greenhouse CO 2 . Biodiesel production from oil crops is seen as currently the best alternative, but still presents the main drawback of competing with food production for arable land. Therefore, production of biodiesel from TAGs present in microalgae can become an environmentally friendly alternative as microalgae produce oil and fix CO 2 from atmosphere without the necessity of implementing vast arable areas for cultivation [3]. On the other hand, consumption of PUFAs in the human diet can help prevent the development of cardiovascular and mental diseases [4]. Fish are a rich source of PUFAs, but uncontrolled fishing has led to a substantial decrease in the worldwide fish population [5]. Production of PUFAs from microalgae may overcome this problem. On the other hand, sterols from microalgae are important part of the diet for juvenile scallops or prawns in aquaculture hatcheries [6]. Moreover, the high protein content in microalgae makes them a possible fodder for agricultural livestock [7]. In ad...

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“…A sterile 1% zinc chloride (ZnCl 2 ) (Sigma-Aldrich, St. Louis, MO, USA) solution was added to each M. aeruginosa UTEX LB 2385 culture to yield relative ZnCl 2 concentrations: 0 mg/L, 0.1 mg/L, 0.25 mg/L, and 0.5 mg/L (0 µM, 0.734 µM, 1.835 µM, 3.669 µM, respectively), as described previously [50,70]. These ZnCl 2 concentrations were targeted through multiple growth curve analysis from past experiments, with concentrations as high as 10 mg/L ZnCl 2 (data not shown).…”

Section: Experimental Designmentioning

confidence: 99%

Effect of Zinc on Microcystis aeruginosa UTEX LB 2385 and Its Toxin Production

Perez

Chu

2020

Toxins

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Cyanobacteria harmful algal blooms (CHABs) are primarily caused by man-made eutrophication and increasing climate-change conditions. The presence of heavy metal runoff in affected water systems may result in CHABs alteration to their ecological interactions. Certain CHABs produce by-products, such as microcystin (MC) cyanotoxins, that have detrimentally affected humans through contact via recreation activities within implicated water bodies, directly drinking contaminated water, ingesting biomagnified cyanotoxins in seafood, and/or contact through miscellaneous water treatment. Metallothionein (MT) is a small, metal-sequestration cysteine rich protein often upregulated within the stress response mechanism. This study focused on zinc metal resistance and stress response in a toxigenic cyanobacterium, Microcystis aeruginosa UTEX LB 2385, by monitoring cells with (0, 0.1, 0.25, and 0.5 mg/L) ZnCl 2 treatment. Flow cytometry and phase contrast microscopy were used to evaluate physiological responses in cultures. Molecular assays and an immunosorbent assay were used to characterize the expression of MT and MC under zinc stress. The results showed that the half maximal inhibitory concentration (IC 50 ) was 0.25 mg/L ZnCl 2 . Flow cytometry and phase contrast microscopy showed morphological changes occurred in cultures exposed to 0.25 and 0.5 mg/L ZnCl 2 . Quantitative PCR (qPCR) analysis of selected cDNA samples showed significant upregulation of Mmt through all time points, significant upregulation of mcyC at a later time point. ELISA MC-LR analysis showed extracellular MC-LR (µg/L) and intracellular MC-LR (µg/cell) quota measurements persisted through 15 days, although 0.25 mg/L ZnCl 2 treatment produced half the normal cell biomass and 0.5 mg/L treatment largely inhibited growth. The 0.25 and 0.5 mg/L ZnCl 2 treated cells demonstrated a~40% and 33% increase of extracellular MC-LR(µg/L) equivalents, respectively, as early as Day 5 compared to control cells. The 0.5 mg/L ZnCl 2 treated cells showed higher total MC-LR (µg/cell) quota yield by Day 8 than both 0 mg/L ZnCl 2 control cells and 0.1 mg/L ZnCl 2 treated cells, indicating release of MCs upon cell lysis. This study showed this Microcystis aeruginosa strain is able to survive in 0.25 mg/L ZnCl 2 concentration. Certain morphological zinc stress responses and the upregulation of mt and mcy genes, as well as periodical increased extracellular MC-LR concentration with ZnCl 2 treatment were observed. Key Contribution: Zinc stress causes cyanotoxin production increase in M. aeruginosa UTEX LB 2385.

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Physiological effects of nickel chloride on the freshwater cyanobacterium <i>Synechococcus</i> sp. IU 625

Cited by 9 publications

References 18 publications

Influence of hydropower stations on the water microbiota in the downstream of Jinsha River, China

Influence of hydropower stations on the water microbiota in the downstream of Jinsha River, China

Effect of Lignocellulose Related Compounds on Microalgae Growth and Product Biosynthesis: A Review

Effect of Zinc on Microcystis aeruginosa UTEX LB 2385 and Its Toxin Production

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