Physiological effects of gibberellic acid. III. Observations on its mode of action on barley endosperm

Paleg, L. G.

doi:10.1104/pp.36.6.829

Cited by 89 publications

(34 citation statements)

References 8 publications

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“…Auxin also delays senescence of bean pericarp tissue and prevents a decrease in RNA (Sacher 1963). Other growth regulators have been shown to produce similar effects in other tissues (Paleg 1961;Ram Chandra and Varner 1963). ,. The results obtained when slices were treated with 10 p.p.m.…”

Section: Discussionmentioning

confidence: 99%

Reversion of Senescence: Effects of 2,4-Dichlorophenoxyacetic Acid and Indoleacetic Acid on Respiration, Ethylene Production, and Ripening of Banana Fruit Slices

Vendrell¹

1969

Aust. Jnl. Of Bio. Sci.

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SummarySlices cut from green, unripe fruit were treated by infiltration with aqueous solutions of 2,4·dichlorophenoxyacetic acid (2,4·D) and indoleacetic acid (lAA). 2,4.D delayed but increased the size of those peaks in respiration and ethylene production which are induced by cutting; ripening was also delayed. These effects were proportional to concentrations of 2,4.D in the range 1O-L lO-3M. Higher concentrations caused injury.Ripening which may be induced by a 24·hr treatment with 10 p.p.m. ethylene is prevented or delayed in slices treated with 2,4.D. 2,4.D did not prevent a climac· teric·like rise in respiration, but the slices recovered from the effects of ethylene. This recovery is shown by a decline in respiration and ethylene production to normal pre·climacteric rates and maintenance of other green fruit characteristics. The recovery is proportional to concentration of 2,4-D in the range 1O-L lO-3M. Ripening subsequently takes place normally. The effects of lAA were similar to those of 2,4.D but less pronounced.The delay in ripening induced by 2,4.D or IAA contrasts with previous reports that treatments with these compounds by dipping or spraying usually hastened ripening of whole fruit.It is suggested that these compounds, especially 2,4-D, cause reversion of banana tissue to a more juvenile state which is less sensitive to ethylene treatment.

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Section: Discussionmentioning

confidence: 99%

Reversion of Senescence: Effects of 2,4-Dichlorophenoxyacetic Acid and Indoleacetic Acid on Respiration, Ethylene Production, and Ripening of Banana Fruit Slices

Vendrell¹

1969

Aust. Jnl. Of Bio. Sci.

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“…diffusion) then the scutellum appears to have the capacity to produce up to 50 % of the total seed hydrolases (a-ll-a-amylase and cell wall breakdown enzymes) during the first 6 to 7 days of germination and seedling growth at 15 ~ The barley cultivar used in the present experiments is one of the most common malting barley varieties in Denmark (Nordal). Although it has been suggested that the gibberellic acid response of the aleurone layer is essentially nonfunctional at 15 ~ (15,21), preliminary experiments with Nordal barley in this laboratory, indicate that 15 ~ should not be considered a sub-optimal temperature to measure gibberellic acid-induced production of hydrolases from the aleurone of Nordal barley. Therefore for this common malting barley the scutellum is initially the most important producer and disseminator of a-ll-a-amylase and cell wall breakdown enzymes and is responsible for the production of up to one half of these enzymes during germination and early seedling growth.…”

Section: Conclusionmentioning

confidence: 99%

On the relative role of the scutellum and aleurone in the production of hydrolases during germination of barley

Gibbons

1981

Carlsberg Res. Commun.

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The relative roles of the scutellum and the aleurone layer in the production and mediation of both germination-specific a-amylase and cell wall breakdown hydrolases has been examined in vivo following treatment of barley seeds with the plant hormones gibberellic acid and abscisic acid. To facilitate entry of these exogenous hormones, a technique is presented where multiple incisions b~nicks~0 are made in the outer layers of the seed prior to germination. Treatment of seeds with gibberellic acid and abscisic acid effected major changes on the pattern of hydrolase transport. The nature of the changes were such that it is concluded that the scutellum alone is capable of producing and mediating up to 50To of germination specific hydrolases in a commercial European malting barley, germinating at the conventional European malting temperature of 15 ~

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“…Paleg (17) reported that gibberellic acid stimulated the production of a-amylase from barley endosperm. Later, Varner (20) reported that under appropriate conditions it was possible to prevent gibberellic-dependent de novo synthesis of aamylase by nucleic acid analogues and actinomycin D.…”

Section: Methodsmentioning

confidence: 99%

Auxin Stimulation of Ethylene Evolution

Abeles¹

1966

Plant Physiol.

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Sumimary. The stimiiulation of ethyleie production fromii seedling tissue of Phaiscolis TIdlgaris, Heliatithus anniumtlis anid Zca( niavs by growth regulators was inihibited by actinomnycin D and puromycin and to a lesser extent by 2-thiouracil and p-fluorophenylalanine. It is concluded that the mechanism of action of growth regulators on the enhancement of ethylene production is the formation of enzym,es involved in ethylene biogenesis. Thlis paper (lescribes experiments that examiiine the possibiliity that auxin-induced ethylene prodductioln is mediated through the production of specific enzymes required for the enhanced synthesis of ethylene. Materials and MethodsEthylene was measured by gas chromatography as described earlier (1). Seven-day-old etiolated beans (Phiaseolits vuilgaris L. var. Red Kidney), 7-dayol( suniflower (Hcliatnthits (Iainnutis L. var. Russian mammiiioth) anid 4-day-old corn (Zea mays L. var. Burpee's Barbecue Hybrid) were grown oln moist vermiculite in the dark at 250 and a relative humidity of about /70 to 80 %. A red light was turned onl for 3 hours during the third night of growth of corn see(llinigs to inhibit mlesocotyl elongationi.Ethylenie evolution from ithe ifollowing por-tions of bean, sunflower, or corn tissues was measured after incubating the mlaterial in syringes. Each syringe conltained: onle 2-cmll beanl hypocotyl harvested immediately below the cotyledonary node and sliced into 1-mmlii sections, or, two 1.4-mm sunflower hypocotyls cut below the cotyledonary node and sliced into 1-mim sections, or tour 2-cm corn coleoptile sections cut 2 mm below the tip of the seedling. These tissues were placed in,to 5-ml syringes, sealed with rubber vaccine caps. in 1 ml of liquid phase and a gas phase of 3 nml. The syringes were shaken 80 times per miniute with an amplitude of 2 cm at 250, and theln 2 mil of the gas phase was withdraxvin for ethvlenie miieasurements. Since, as reported earlier (1), a 2-hour lag period occurs between addition of auxin and stimulation of ethylene production, syringes were vented after 2 hours, sealed, and ethylene was measured after 2 additionial hours of shaking. Each ex-585 www.plantphysiol.org on May 11, 2018 -Published by Downloaded from

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Physiological effects of gibberellic acid. III. Observations on its mode of action on barley endosperm

Cited by 89 publications

References 8 publications

Reversion of Senescence: Effects of 2,4-Dichlorophenoxyacetic Acid and Indoleacetic Acid on Respiration, Ethylene Production, and Ripening of Banana Fruit Slices

Reversion of Senescence: Effects of 2,4-Dichlorophenoxyacetic Acid and Indoleacetic Acid on Respiration, Ethylene Production, and Ripening of Banana Fruit Slices

On the relative role of the scutellum and aleurone in the production of hydrolases during germination of barley

Auxin Stimulation of Ethylene Evolution

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