Physiological Dynamism of Various Serum Cardiac Biomarkers in Apparently Healthy Sipli Sheep of Pakistan

Abstract

The present work is the first record of deducing normal reference intervals (RIs) for various serum cardiac biomarkers namely cardiac troponin I (cTnI), alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase-myocardial band (CK-MB), sodium (Na+), potassium (K+) and total protein (TP), and their interrelationship in apparently healthy indigenous Sipli breed of sheep (n = 141) from Pakistan. Apropos to aseptic blood collection and serum extraction, the attributes were deduced using commercially available kits. For the sake of analyses, the studied biomarkers were attributed as independent (Na+, K+, and TP) and dependent (LDH, ALT, AST, cTnI and CK-MB) variables. The RIs were determined through Reference Value Advisor Version 2.1 as per gender and age keeping in view the guidelines provided by American Society of Veterinary Pathology (ASVCP). Results revealed that the RIs for cardiac serum biomarkers included in the present study were generally within the reference range provided for sheep with slight variations which might be attributed to physiological adaptation to harsh desert climate. The Na+ significantly (P ≤ 0.05) predicted AST (adj. r-square = 0.976) and CK-MB (adj. r-square = 0.477). Similarly, K+ significantly (P ≤ 0.05) predicted LDH (adj. r-square = 0.626) and ALT (adj. r-square = 0.897). The TP had highest adjusted r-square of 0.770 with AST however it was statistically non-significant (P ≥ 0.05). The results of the study present a baseline data about these cardiac biomarkers which may be utilized for cardiac assessment of the sheep. It is recommended that the serum cardiac biomarkers ascertained in this study may be studied along with electrophysiology and ultrasonography of sheep heart for confirmed diagnosis/prognosis of cardiomyopathies.