Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process

Duarte-Aké, Fátima; Castillo-Castro, Eduardo; Pool, Felipe Barredo; Espadas, Francisco; Santamaría, Jorge M.; Robert, Manuel L.; De‐la‐Peña, Clelia

doi:10.1007/s00299-016-2049-0

Cited by 38 publications

(34 citation statements)

References 74 publications

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“…Abnormalities in plantlets induced by epigenetic factors have been reported in different crops (Kitimu et al 2015;Ghosh et al 2017;Goyali et al 2018). In Agave angustifolia Haw., albinism was related to the tissue culture environment where the missing of color in leaves was correlated with high levels of DNA methylation (Duarte-Aké et al 2016). In Elaeis guineensis Jacq.…”

Section: Crop Specie Somaclonal Variation Type Causes Of Variation/anmentioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

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Somatic embryogenesis is a morphogenetic event where somatic cells have the ability to produce embryos without gamete fusion. It is used as a technique for plant mass propagation. It is a process that has six well defined steps such as induction, expression, development, maturation, germination and plant conversion. These steps are characterized by distinct physiological, morphological and molecular events. Although somatic embryogenesis has been established in several plant species, there remains many problems to be solved. The main problem in somatic embryogenesis is the large number of abnormal embryos produced which cannot germinate nor convert into normal plants. Abnormalities in somatic embryos (SE) can be generated by genetic or epigenetic changes in the DNA. These changes in the DNA can be influenced by external factors such as the use of plant growth regulators and mutagenic substances or stress factors applied to the plant tissue such as high and low temperatures, drought, salinity, and heavy metals. Abnormalities generated by genetic changes in the DNA are hardly reversible; however, abnormalities generated by epigenetic changes may be reversible and the abnormal embryos are able to produce normal plants in most cases. This review focuses on the identification of the main factors that can cause abnormal SE development in different plant species, suggest how SE abnormalities are related to somaclonal variations and identify which genes may be involved with embryo abnormalities. Zygotic embryo abnormalities in Arabidopsis thaliana mutants are listed with the aim to understand the main genetic mechanisms involved in embryo aberrations. Key messageThe abnormalities in somatic embryos are related to the use of 2,4-D in most of the published protocols, this sintetic auxin disrupts the endogenous auxin balance and the auxin polar transportation interfering with the embryo apical-basal polarity.

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Section: Crop Specie Somaclonal Variation Type Causes Of Variation/anmentioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

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“…Albino plantlet regeneration is associated with aberrant plastid formation and plastidic DNA deletions [74,75], altered expression of photosynthesis, porphyrin and chlorophyll metabolism genes [67], global DNA methylation changes [76], and modified physiology [76,77]. Furthermore, QTL studies have identified 14 chromosome regions associated with albinism in triticale [78], which suggested a role for oxidative stress during the proplastid to functional chloroplast transition in the promotion of albinism during in vitro regeneration.…”

Section: Albino Regenerationmentioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2020

Plants

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Sorghum has been considered a recalcitrant plant in vitro and suffers from a lack of regeneration protocols that function broadly and efficiently across a range of genotypes. This study was initiated to identify differential genotype-in vitro protocol responses across a range of bioenergy sorghum parental lines and the common grain sorghum genotype Tx430 in order to characterize response profiles for use in future genetic studies. Two different in vitro protocols, LG and WU, were used for comparisons. Distinct genotype-protocol responses were observed, and the WU protocol performed significantly better for plantlet regeneration. Most bioenergy genotypes performed as well, if not better than Tx430, with Rio and PI329311 as the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. Genotype- and protocol-dependent albino plantlet regeneration was also noted, with three of the bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were susceptible to albino plantlet regeneration, with the response strongly associated with the WU protocol. These bioenergy parental genotypes, and their differential responses under two in vitro protocols, provide tools to further explore and assess the role of genetic loci, candidate genes, and allelic variants in the regulation of in vitro responsiveness in sorghum.

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“…Albino plantlet regeneration is associated with aberrant plastid formation and plastidic DNA deletions (Day and Ellis 1984, Dunford and Walden 1991), altered expression of photosynthesis, porphyrin and chlorophyll metabolism genes (Zhao et al 2017), global DNA methylation changes (Duarte-Aké et al 2016), and modified physiology (Duarte-Aké et al 2016; Isah 2019). Furthermore, QTL studies have identified 14 chromosome regions associated with albinism in triticale (Krzewska et al 2015), which suggested a role for oxidative stress during the proplastid to functional chloroplast transition in the promotion of albinism during in vitro regeneration.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2019

Preprint

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25Sorghum has been considered a recalcitrant plant in vitro, and suffers from a lack of 26 regeneration protocols that function broadly and efficiently across a range of genotypes. This study 27 was initiated to identify differential genotype-in vitro protocol responses across a range of 28 bioenergy sorghum bioenergy parental lines, in order to characterize response profiles for use in 29 future genetic studies. Seven bioenergy sorghum genotypes were compared, along with the 30 common grain sorghum genotype Tx430, for their in vitro regeneration responses using two 31 different in vitro protocols, LG and WU. All genotypes displayed some level of response during 32 in vitro culture with both protocols. Distinct genotype-protocol responses were observed, with the 33 WU protocol significantly better for plantlet regeneration. All bioenergy genotypes, with the 34 exception of Chinese Amber, performed as well, if not better than Tx430, with Rio and PI329311 35 the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic 36 exudation responses, as indicated by medium browning. During the callus induction phase, 37 genotypes prone to medium browning exhibited a response on WU medium which was either equal 38 or greater than on LG medium, with Pink Kafir and PI329311 the most prone to medium browning. 39 Genotype-and protocol-dependent albino plantlet regeneration was also noted, with three of the 40 bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were 41 susceptible to albino plantlet regeneration, with the response strongly associated with the WU 42 protocol. Pink Kafir displayed the highest albino formation, with close to 25% of regenerating 43 explants forming albino plantlets. 44 45 46 47 48 49 50 51 52 53 Sorghum [Sorghum bicolor (L.) Moench] ranks fifth of the major grain crops in production, 54 area harvested, and yield worldwide (FAOSTAT Database 2017), and more than 300 million 55 people use it as a staple food, particularly in developing semiarid tropical regions (Kebede et al.56

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Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process

Cited by 38 publications

References 74 publications

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Comparative Analysis of In Vitro Responses and Regeneration Between Diverse Bioenergy Sorghum Genotypes

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